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271.
Evaluation of Commercial Kits for Dual Extraction of DNA and RNA from Human Body Fluids, , 下载免费PDF全文
Andrew J. Schweighardt Ph.D. Courtney M. Tate Ph.D. Kristina A. Scott M.S. Kathryn A. Harper M.S. James M. Robertson Ph.D. 《Journal of forensic sciences》2015,60(1):157-165
STR typing of DNA evidence can identify the donor with a high power of discrimination but cannot identify the tissue origin of a body‐fluid stain. Using RNA to attribute a crime scene stain to a particular tissue may aid in reconstruction efforts. With blood from 10 donors, four DNA and RNA coextraction kits were evaluated by measuring yields and STR and mRNA profiles. T tests indicated some significant differences in kit performance. The Zymo Research ZR‐Duet? kit performed best based on average DNA (41.4 ng) and mRNA (4.07 ng) yields and was the only kit to provide complete DNA/RNA profiles for all samples. The consistency of this kit was challenged by data from additional blood and saliva donors. Further testing is advised before a superior kit is unequivocally chosen. Stand‐alone DNA or RNA purification generally offers higher yield, but coextraction may still allow successful STR profiling and tissue source identification. 相似文献
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273.
The Dynamics of Self-Esteem: A Growth-Curve Analysis 总被引:2,自引:0,他引:2
Research on adolescent self-esteem has been inconsistent regarding development patterns and processes, with some scholars concluding that self-esteem is a static construct and others concluding that it is a dynamic construct. A potential source of this inconsistency is the lack of attention to intraindividual changes in self-esteem across adolescence and to gender-specific developmental patterns. Building on previous research, we use a growth-curve analysis to examine intraindividual self-esteem changes from early adolescence to early adulthood. Using 7 years of sequential data from the Family Health Study (762 subjects ages 11–16 in Year 1), we estimated a hierarchical growth-curve model that emphasized the effects of age, life events, gender, and family cohesion on self-esteem. The results indicated that age had a curvilinear relationship with self-esteem suggesting that during adolescence self-esteem is a dynamic rather than a static construct. Furthermore, changes in self-esteem during adolescence were influenced by shifts in life events and family cohesion. These processes were different for males and females, particularly during early adolescence. 相似文献
274.
Hilda Scott 《Women's studies international forum》1982,5(5):451-462
In this paper I shall review some of the theoretical obstacles which the classical Marxist analysis of women's subordination placed in the path of the socialist movement when it attempted to solve the question of women's inequality in practice. I will also show how the particular path of socialist construction that the Eastern European countries chose helped to perpetuate the traditional division of roles between men and women and to create a chain reaction which appears to be impervious to the demands of ideology. 相似文献
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276.
Cooper GA Allen DL Scott KS Oliver JS Ditton J Smith ID 《Journal of forensic sciences》2000,45(2):400-406
Drug use histories were collected from 100 subjects recruited from the "dance scene" in and around Glasgow, Scotland. In addition, each subject donated a hair sample which was analyzed by gas chromatography/mass spectrometry (GC/MS) for amphetamine (AP), methamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MD MA) and 3,4-methylenedioxyethylamphetamine (MDEA). The hair samples were analyzed in two 6 cm segments or in full, ranging from 1.5 to 12 cm depending on the length of the hair. Approximately 10 mg of hair was ground to a fine powder before treatment with beta-glucuronidase/aryl sulfatase. A solid-phase extraction procedure was carried out followed by derivatization with pentafluoropropionic anhydride (PFPA). All extracts were analyzed by gas chromatography/mass spectrometry (GC/MS). Of the 139 segments analyzed, 77 (52.5%) were positive for at least one of the five amphetamines. The drug concentrations found in the hair were compared with the self-reported drug histories. A concordance of greater than 50% was found between the self-report data and levels detected in hair. However, no correlation was found between the reported number of "ecstasy" tablets consumed and the drug levels detected in hair. An increase in the average drug levels measured was observed from low to high use (number of "ecstasy" tablets/month). A large number of false negatives and a low number of false positives were observed. 相似文献
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278.
Offshore wind farms are likely to be a key component of theUnited Kingdoms strategy for renewable energy, and theEnergy Act 2004 introduced a new legislative framework for thedevelopment of wind farms beyond the limits of territorial seawithin designated renewable energy zones. The compatibilityof the new arrangements with international marine and environmentalobligations is analysed. Rights of navigation, the protectionof the marine environment and the decommissioning of turbinesat the end of their operational life all raise significant legalissues. The introduction of the Energy Act is an important developmentin assisting the construction and operation of offshore windfarms. But the law was passed at a time when a number of majorreviews had concluded that the legislative and policy frameworkfor the protection of the marine environment suffered severeweaknesses. It is unfortunate that the UK Government did notconsider the creation of an offshore renewable energy industryas an opportunity to develop and apply the sort of new marineconservation strategies and legal frameworks being advocatedas essential in this area. 相似文献
279.
The post-1999 devolution project has resulted in a major recalibrationof the preexisting arrangements for making European Union policywithin the United Kingdom. The devolved administrations in Scotlandand Wales (but not the English regions) have gained in electorallegitimacy and legislative powers, and thereby have a greaterclaim to consultation with UK central government. Four key characteristicsof European Union policymaking in a devolved United Kingdomare identified. The legal contingency of the "devolveds" statushas not yet impeded traditional cooperative relations betweengovernment tiers, but the stability of the new arrangementsremains in question. The UK case is compared with European Unionregionalization in other member states and a distinction isdrawn between a cooperative regionalist approach (the devolveds)and a consultative one (the English regions). 相似文献
280.