A simple DNA extraction method for marijuana samples used in amplified fragment length polymorphism (AFLP) analysis

As a first step in developing a molecular method for the individualization of marijuana samples, we evaluated a plant DNA extraction kit. The QIAGEN plant DNeasy method uses a spin column format for recovery of DNA and is effective for obtaining high molecular weight DNA from leaf, flower (bud), and seed samples of marijuana. The average DNA yield was 125-500 ng per 100 milligrams of fresh plant tissue. The recovered DNA was of polymerase chain reaction (PCR) quality as measured by the ability to generate reproducible amplified fragment length polymorphism (AFLP) profiles. AFLP is a technique used to create a DNA profile for plant varieties and is being applied to marijuana samples by the authors to link growers and distributors of clonal material. The QIAGEN plant DNeasy method was simple, efficient, and reproducible for processing small quantities of marijuana into DNA.     

Asserting integrity in Mexico's civic sector

In societies where civic space is closing, integrity in the civic sector is critical for its sustainability. Where state regulatory frameworks are inadequate, or worse, manipulative, self-regulation can help defend the sector's integrity and strengthen the ability of civic associations to serve the public and contribute to democracy. This paper describes the strategic role in self-regulation of a particular type of third sector actor in Mexico, the coordinating body or civic network. A case study of the Mexican Center for Philanthropy (CEMEFI) and its Accreditation in Institutionalization and Transparency project illustrates the contribution of NGO networks in service to the sector, and their key role in diffusion of norms of transparency and accountability within the civic sector and beyond.     

General Motor Holden's closure in Playford,South Australia: Analysis of the policy response and its implications for health

Deindustrialisation and the closure of automotive manufacturing can differentially affect the socioeconomic prospects of workers and their communities, and contribute to social and health inequity. We used Bacchi's problematisation approach to examine the South Australian (SA) Labor government's policy responses to the General Motors Holden (GMH) Elizabeth plant closure announcement. We focused on the way that these policy responses framed the ‘problem’ of this major economic shock, particularly the extent to which potential social and health equity consequences were addressed. We found a narrow focus on economic strategies, neglecting the compounding impact of poverty in Playford, which may exacerbate health inequity. The community effects from the GMH closure remain uncertain and may be delayed for several years. SA requires better integrated social and economic policies to minimise social and health inequalities, as the consequences of the car manufacturing loss are realised.     

Two heads are better than one? How to effectively use two interviewers to elicit cues to deception

Background. We examined the effect of a second interviewer's demeanour on cues to deception. We predicted that a supportive demeanour would be the most beneficial for eliciting verbal cues to deceit, as it would encourage truth tellers, but not liars, to say more. In addition, we examined the extent to which interviewees deliberately made eye contact with the interviewers. Liars take their credibility less for granted than truth tellers, and therefore have a greater drive to be convincing. Liars are thus more likely to monitor the interviewer to determine if the interviewer appears to believe them. Method. Participants appeared before two interviewers: the first asked all the questions and the second remained silent. The second interviewer exhibited either a supportive, neutral, or a suspicious demeanour. Results. Truth tellers provided significantly more detail than liars, but only in the supportive second interviewer condition. The effect of a second interviewer's demeanour on detail was perhaps remarkable given that the interviewees hardly looked at the second interviewer (less than 10% of the time). Liars displayed more deliberate eye contact (with the first interviewer) than truth tellers did. Conclusions. A supportive second interviewer has a positive effect on interviewing. We discuss this finding in the wider contexts of investigative interviewing and interviewing to detect deception.     

Two Approaches,One Course: An Experience in Experiential Learning

In universities where experiential learning is not the norm, introducing this style of learning into undergraduate courses can be an intimidating process for both instructors and students. Instructors are often unsure of how to manage student experiences in the community, while a significant number of students react with skepticism toward this new type of course, as well as concern about their instructor's changing expectations for their performance. The following is a reflection of our first 2 years of teaching undergraduate courses from a distinctly experiential learning approach. Qualitative data is used to highlight the parallel learning processes that occurred over the semester, for students as well as for us as instructors. Our biggest challenges are explored in detail, and advice to instructors contemplating adapting an experiential approach to their own courses is presented.     

School Smoking Policy Characteristics and Individual Perceptions of the School Tobacco Context: Are They Linked to Students’ Smoking Status?

The purpose of this study was to explore individual- and school-level policy characteristics on student smoking behavior using an ecological perspective. Participants were 24,213 (51% female) Grade 10–11 students from 81 schools in five Canadian provinces. Data were collected using student self-report surveys, written policies collected from schools, interviews with school administrators, and school property observations to assess multiple dimensions of the school tobacco policy. The multi-level modeling results revealed that the school a student attended was associated with his/her smoking behavior. Individual-level variables that were associated with student smoking included lower school connectedness, a greater number of family and friends who smoked, higher perceptions of student smoking prevalence, lower perceptions of student smoking frequency, and stronger perceptions of the school tobacco context. School-level variables associated with student smoking included weaker policy intention indicating prohibition and assistance to overcome tobacco addiction, weaker policy implementation involving strategies for enforcement, and a higher number of students smoking on school property. These findings suggest that the school environment is important to tobacco control strategies, and that various policy dimensions have unique relationships to student smoking. School tobacco policies should be part of a comprehensive approach to adolescent tobacco use.     

Estimates of Repeat Victimization Using the National Crime Victimization Survey

Many crime victims experience multiple victimizations over time. Estimating the rate of repeat victimization from a longitudinal survey, however, is difficult because individuals often have missing data for some of the interviews. We use data from the 1996–98 U.S. National Crime Victimization Survey to explore potential effects of missing data on estimated rates of repeat violent victimizations in individuals. We introduce two algorithms for estimating repeat victimization rates, using logistic models to impute values for individuals who have partial data. These models are applied to estimate rates of repeat victimization for all violent crimes, and separately for crimes of domestic violence. Estimates show substantial sensitivity to the form of the model used.