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101.
Preparation of degraded human DNA under controlled conditions 总被引:7,自引:0,他引:7
DNA typing through analysis of short tandem repeats (STRs) and mitochondrial DNA (mtDNA) by means of the polymerase chain reaction (PCR) and sequencing are the common methods for the forensic identification of persons and reconstruction of kinship, especially when skeletal human remains have to be analyzed. Furthermore, samples typically found at crime scenes may be both quantitatively and qualitatively inadequate since they may contain very scarce and often degraded DNA due to exposure to heat, light, humidity, and microorganisms. In order to improve the performance of STR typing technology in those cases where DNA availability is limited, it would be desirable to have a source of degraded DNA with known properties. For this purpose, we have developed a method to prepare artificially degraded DNA under controlled conditions. By treatment of genomic DNA with sonication and DNAse I we have produced DNA fragments within a defined range of lengths. STR typing of this degraded DNA with a commercially available multiplex kit could only produce partial profiles as indicated by the absence of STR alleles with sizes >200 bp. This artificially degraded DNA can be used for the improvement and standardization of STR typing protocols when only highly degraded DNA is available for analysis. 相似文献
102.
Christopher J. Schneider 《Critical Criminology》2004,12(1):87-103
This paper begins with an overview of criticalrace theory tenets, which in turn will befollowed by an overview of postmodernism. These bodies of knowledge consist of an arrayof ideologies; but for the purpose of thisarticle only the fundamentals of each will bediscussed. Thereafter, an integration ofthese two contemporary areas of thought willdemonstrate the constructive linkage ofcritical race theory and postmodern theory. Theintegrations that follow will emphasize how acollaboration of critical race theory tenetsand postmodern thought can contribute to asuccessful, and more importantly improved,analysis of the social constructions of race,class, and gender. In addition, theintersectional analysis presented willdemonstrate a more informative and betterunderstanding of the subtleties of blatant andmore hidden forms of race, class, and gender. 相似文献
103.
In the aftermath of an election it has become customary to systematically compare the predictive accuracy of polls and political stock exchanges. However, most of these analyses do not emerge from real predictions. In this research note, we compare the predictive performance of a time-series model to the accuracy of polls and a political stock market in the 2005 german Bundestag (general) election. Our analysis shows that Wahl$treet, the most significant german political stock exchange, was again closest to the actual results leaving commercial polling institutes behind. Due to the exceptionally large errors of most institutes we briefly discuss how such inaccurate forecasts might affect voting behaviour in the light of social choice theory. 相似文献
104.
Simone Browne 《Citizenship Studies》2005,9(4):423-438
This article is concerned with the ways in which border control has been reconstituted through Canada's Permanent Resident Card (PRC). Some questions examined with this paper include: how did the PRC come to exist as a technology of border control? Does it function as a symbol of the Canadian nation-state's imperative to manage transnational movement and access to the geopolitical space of the nation and, if so, how? Through what means does the PRC and the events surrounding its introduction and use facilitate processes of serialization and racialization? Does the PRC, as a technique of reason of state, do the work of producing the category “responsible immigrants”? The notions of “economies of bodies” and “bordering” are important here. “Bordering” opens up the concept of the border from a fixed place to a verb, or a process. Given this, bordering does not only occur at the territorial boundaries of the nation-state, it can also be internal to it. By examining how the category of “permanent resident” is organized, gains meaning and is maintained, this article demonstrates how the technology of the PRC and similar technologies of the regulation of mobility operate as practices of bordering and nation-making and constitute Canadian citizenship. 相似文献
105.
Schneider J 《U.S. news & world report》2003,134(19):58,60
106.
Governance at international and global levels is not only provided through states and markets but also through a variety of private organizations. The business world is well represented through this kind of organization and contributes to global governance through self-regulation across a number of industries. This article examines these efforts in the encompassing organization of global commerce, in the pharmaceutical industry and among dye stuffs producers. Smaller organizations are generally better suited to monitor compliance and impose sanctions on members violating the codes and norms behind self-regulation. Even small organizations, however, are confronted with problems and there is also evidence of large and very complex organizations having established effective mechanisms as alternatives to public regulation. These experiences can be built into theories on self-regulation as a form of global governance. 相似文献
107.
Mark Schneider 《Public Choice》1986,48(3):255-263
An assumption of the post-World War II metropolitan reform movement was that fragmentation of metropolitan regions into multiple local governments was wasteful and inefficient, increasing the cost and size of government. More recently, ‘polycentrists’ have argued that the competition between multiple governments in metropolitan regions can in factreduce the growth in government by providing a competitive check on the excessive demands of local bureaucrats for more resources. In this article, I explore the effects of fragmentation on growth in the size of suburban municipal government budgets and in the number of services offered. Competition inherent in more fragmented metropolitan regions is shown to slow the expansion in local government expenditures and service levels. 相似文献
108.
Schneider CE 《Indiana law journal (Indianapolis, Ind. : 1926)》1994,69(4):1075-1104
109.
We propose an alternativeto the Constitutional Treaty drafted by theEuropean Convention. Our proposaleffectively limits the domain of governmentat the Union level. It takes the incentivesof the European public actors into account.We propose a second chamber of EuropeanParliament composed of delegates of theparliaments of the member states and asecond court composed of delegates of thehighest courts of the member states. Theseinstitutions will be the guardians of thesubsidiarity principle. The principle ofthe separation of powers is implemented byabolishing the Commission's monopoly oflegislative initiative and by curtailingthe Council's legislative role. Treatyamendments will not be drafted by aninter-governmental conference but by aninter-parliamentary conference. We proposeto change the method of financing andintroduce referenda. 相似文献
110.
Simone Wong 《Feminist Legal Studies》2003,11(2):119-137
In July 2002, the U.K. Law Commission published its Discussion Paper No.287 on home-sharing. The conclusion drawn by the Law
Commission was that it would not be possible to devise a statutory scheme for the resolution of family property disputes which
is both workable and flexible enough to deal with the wide range of personal relationships that exist. It further took the
view that, with appropriate changes to the way in which trusts principles are currently interpreted and applied by the courts,
these trusts principles are sufficiently flexible and coherent to deal with the question of ascertaining and quantifying property
rights over the family home. The aim of this paper is to examine the implications of these particular conclusions drawn by
the Law Commission for both the law of trusts and the resolution of family property disputes between cohabitants. In particular,
the paper will consider the extent to which trusts law remains a workable and desirable option and whether any mileage may
be gained by drawing on the human rights culture that is emerging in U.K. legal and political discourse.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献