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981.
Public policymakers and regulators worldwide are grappling with the desire to improve environmental quality through appropriate regulation of business, while also streamlining government. Concurrently, environmentally conscience consumers are calling for improved environmental performance by industry. As a result of these pressures, regulators and lawmakers worldwide are attempting to craft effective policies that create adequate incentives for environmental protection on the part of firms, in the face of decreasing budgets and an increased demand for the use of market‐based incentives. To aid decision makers as they struggle with these concerns, this study provides a detailed case examination of the dilemmas and responses of national‐level regulators as they try to develop appropriate responses to the rise of international and “voluntary” management regimes. To accomplish these goals, this article compares the public policy responses of governments around the world to one such voluntary international environmental regime: ISO 14001. ISO 14001 is a form of industry self‐regulation in response to market forces calling for harmonization in environmental management and as a result of consumer and trade‐partner demands. This study examines the relationships between regulators and the regulated in order to understand if ISO 14001 certified firms are receiving regulatory relief or other forms of public policy/regulatory benefits as a result of their certification. It will also examine the impact that government incentives (or their absence) are having on the certification decisions of firms around the world. This information helps us to begin to understand how the trends toward smaller government and voluntary environmental regimes are affecting one another.  相似文献   
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Roma Flinders Mitchell, 1913–2000, AC, DBE, Queen's Counsel, Judge, Founding Chair of the Human Rights Commission, Chancellor of the University of Adelaide, Governor of South Australia, was the first woman in Australia—often also the first in the British Commonwealth—to gain the positions and honours that gild any narrative of her life. How did she do this? What was it like for her? Such questions follow immediately. And then, making it more complicated, what else was there in her life besides achievements and honours? What other doors opened before her as she moved through her days? And what doors closed? How did she choose some doors and not others?

These are questions that we are addressing in our biography of Roma Flinders Mitchell. They are not questions that we will consider here, though. Rather, our subject in this article is not our narrative but Roma Mitchell's own story of her life. It develops into a three‐stranded narrative, composed principally of interviews for press and television, predominantly during the last decades of her life. It is therefore a story shaped by her recognition of herself as exceptional, ‘Roma the First’, and also by other people's desires that she be—for them—precisely that: ‘Roma the First’. This is the ‘authorised’ narrative of Roma Mitchell's life, the story that she told herself. Many regard it as unquestionably definitive, and therefore determining. Yet, it does, itself, prompt an array of questions.

The story is set in Australia, ‘the last of lands, the emptiest’ wrote poet A.D. Hope, in a time that historian Michael Ignatieff deemed ‘“the worst century there has ever been,” in wanton destruction of human life and in murderous unreason masking itself as reason’. More specifically, it takes its beginning from the early years of the twentieth century, in the city of Adelaide, core of a British colony founded less than a century earlier, on the plain that had basked in the custodianship of the Kaurna people before the arrival of the ships from Britain, roughly in the middle of the southern curve defining the Australian continent. The forms that appear in the story derive from those origins: the British law which the colonists practised, with all its theatrical paraphernalia and terminology; a gradually developing copy of Westminster government, but with deference to British rule and allegiance always observed; education adopted from schools and universities in England and Scotland. Only in its churches was it distinct, for, among all of Britain's colonies in Australia, South Australia was the ‘paradise of dissent’. Here, on the edge of the anglophone world, this story did not so much unfold as gather itself together and take off.  相似文献   

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Robotic systems are commonly utilized for the extraction of database samples. However, the application of robotic extraction to forensic casework samples is a more daunting task. Such a system must be versatile enough to accommodate a wide range of samples that may contain greatly varying amounts of DNA, but it must also pose no more risk of contamination than the manual DNA extraction methods. This study demonstrates that the BioMek 2000 Laboratory Automation Workstation, used in combination with the DNA IQ System, is versatile enough to accommodate the wide range of samples typically encountered by a crime laboratory. The use of a silica coated paramagnetic resin, as with the DNA IQ System, facilitates the adaptation of an open well, hands off, robotic system to the extraction of casework samples since no filtration or centrifugation steps are needed. Moreover, the DNA remains tightly coupled to the silica coated paramagnetic resin for the entire process until the elution step. A short pre-extraction incubation step is necessary prior to loading samples onto the robot and it is at this step that most modifications are made to accommodate the different sample types and substrates commonly encountered with forensic evidentiary samples. Sexual assault (mixed stain) samples, cigarette butts, blood stains, buccal swabs, and various tissue samples were successfully extracted with the BioMek 2000 Laboratory Automation Workstation and the DNA IQ System, with no evidence of contamination throughout the extensive validation studies reported here.  相似文献   
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The PowerPlex 16 BIO multiplex short tandem repeat (STR) system contains the 13 CODIS loci (FGA, TPOX, D8S1179, vWA, D18S51, D21S11, TH01, D3S1358, CSF1PO, D16S539, D7S820, D13S317, and DS5S818), plus two pentanucleotide repeat loci (Penta D and Penta E) and the sex-identifying locus. Amelogenin. The PowerPlex 16 BIO System is optimized for use with the Hitachi FMBIO gel imaging systems. A consortium of seven independent laboratories collaborated to perform the studies defined by the FBI standards for performing a developmental validation, including the evaluation of sample concordance, percent stutter determination, nonprobative casework, precision, sensitivity, mixture determination, effect of substrates, the impact of environmental insults, and species specificity. All samples tested for concordance were consistent except for one sample from the Virginia Division of Forensic Science database that displayed discordance at D13S317, a locus whose primer sequence was altered. Stutter values were comparable to those of other STR multiplex systems, the precision was comparable to other multiplexes analyzed by gel electrophoresis, the DNA profiles were unchanged by the substrate upon which the blood samples were placed, and the nonprobative casework samples re-typed for the PowerPlex 16 BIO System were consistent with previous typing results. When greater than 0.125 ng of DNA was placed into the PowerPlex 16 BIO System amplification reaction, a full profile was generated by all laboratories. The mixture study results were comparable to those reported for other multiplex systems, the environmental study demonstrated a loss of larger molecular weight loci when samples were incubated at elevated temperatures for a prolonged period of time, and the only notable cross species hybridization was observed with primate DNA samples. This extensive validation work performed demonstrates that the PowerPlex 16 BIO System provides STR data of a quality comparable with other PowerPlex STR multiplex kits as well as other widely used STR multiplexes and is thus suitable for evidentiary casework analysis as well as database sample profiling.  相似文献   
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