两种DNA提取法对不同色泽肋软骨STR分型成功率的比较

目的比较两种DNA提取法对不同色泽肋软骨的DNASTR分型结果的影响。方法利用Chelex-100法和酚-氯仿法,分别对30例不同色泽的腐败尸体肋软骨进行DNA提取,STR复合扩增,ABI3100型基因分析仪对扩增产物进行检测。结果用酚-氯仿法提取的30例腐败尸体肋软骨,均检测到全部STR基因座的等位基因型。用Chelex-100法提取的肋软骨中,22例(11例白色、8例淡黄色、3例黄色)检测出全部STR基因座的等位基因型;7例(3例黄色、4例黄褐色)检测出部分STR基因座的等位基因型;1例黑灰色的腐败尸体肋软骨,未检测出STR基因座的等位基因型。结论根据肋软骨的色泽,选择适宜的DNA提取方法。对于颜色较深的肋软骨,用酚-氯仿法进行DNA提取有助于提高其STR基因座的检出率。     

中国知识产权法律与国际条约的关系

知识产权的保护各国都订有法律加以规范,但各国给予的保护只在本国境内有效,而知识产权法所保护的客体,即发明、商标、作品等是无形的,很容易依附于各种载体而流往国外,因此也还需要在国外获得保护。为了便利本国国民在外国获得对其发明、商标、作品等的保护,一些国际条约先后签订,规定参加条约的国家互相给予其他缔约国国民以本国国民的待遇。我国已经加入了部分条约,也兼顾了本国法律与国际条约的衔接,但从我国目前的知识产权法律现状看,两者间还存在一些问题。对我国的这些法律与国际条约的关系予以探讨,达到二者间的平衡与完善十分必要。     

科学发展观:妇女发展的灵魂

科学发展观是马克思主义发展观与当代中国发展实际相结合的产物。从性别视角来分析,科学发展观的提出坚持了马克思主义妇女发展的基本观点,彰显出鲜明的促进妇女发展的时代精神,确立起审视妇女发展的新的坐标,提供了推进妇女发展的思想武器,是妇女发展的灵魂之所在。     

Real Rules for Virtual Space

<正>The international community struggles to find common ground on cyber security More than 700 representatives from 60 countries London Conference on Cyberspace, hosted by the British Foreign and Common wealth Office.     

Moving Pictures On the Move

<正>China’s animation industry speeds up Among the dazzling Chinese New Year films this year, several big-budget animations were screened almost at     

Hunting the Wild Man

<正>Scientists and volunteers plan a new Shennongjia exploration for Bigfoot After being shelved for many years, a plan to search for the wild man in the Shennongjia forestry district is once again under way. This time, scientists want to raise as much as 10 million yuan ($1.6 million) to employ advanced technology and recruit staff worldwide for the project.     

Learning From Low Budgets

<正>Chinese filmmakers turn small-budget productions into box-office successes Organizers of China’s upcoming film festivals are finally giving recognition to the little guys—low budget films—to encourage a generation of young,talented directors.     

Back to the Wild

<正>After decades of captive breeding,wild horses survive on their own in northwest China’s nature reserve Seven wild horses raised in captivity were released into the West Lake National Nature Reserve in Dunhuang,northwest China’s Gansu Province, on September 25, 2010.     

Rescue Rangers

<正>Chinese rescuers rush to assist disaster-stricken countries Avideo clip on Liu Xiangyang’s computer displays a moment he is very proud of. The clip shows an Iranian boy running after some vehicles, holding China’s national flag in his hands and chanting China, China...     

The Mellowing of Hollywood

IN 2010,China’s cinema box office surpassed RMB 10 billion,some 2 billion of which was earned by two Hollywood blockbusters - 2012 and Avatar.They both incorporated Chinese elements that Chinese audiences found quite gratifying.