艾灸对腹泻型肠易激综合征模型大鼠海马与结肠组织中IKKβ/IKBα/NF-κB通路的影响

目的观察艾灸对腹泻型肠易激综合征(diarrhea predominant irritable bowel syndrome,IBS-D)模型大鼠海马与结肠组织中核因子κB抑制蛋白(inhibitor of nuclear factor kappa B,IκB)激酶β(IκB kinase beta,IKKβ)、核因子κB抑制蛋白α(NF-kappa-B inhibitor alpha,IKBα)、核因子κB(nuclear factor kappa B,NF-κB)(P65)相对表达水平的影响,探讨艾灸治疗IBS-D的机制。方法将24只健康雄性SD大鼠随机分为空白组、模型组和艾灸组,每组8只。采用慢性束缚联合番泻叶灌胃方法建立IBS-D大鼠模型。艾灸组予以艾灸"天枢""上巨虚",每日30min,每日1次,共治疗7d。治疗后,观察大鼠稀便率和直肠扩张所引起腹部回缩反射(abdominal withdrawal reflex,AWR)的最小容量阈值;蛋白质印迹法检测海马和结肠组织中IKKβ、IKBα、NF-κB(P65)蛋白表达水平。结果与空白组比较,模型组AWR的最小容量阈值明显下降(P<0.05),稀便率明显升高(P<0.05);海马和结肠组织中IKKβ、IKBα、NF-κB(P65)蛋白表达水平明显增加(P<0.05)。与模型组比较,艾灸组AWR的最小容量阈值明显升高(P<0.05),稀便率明显下降(P<0.05);海马和结肠组织中IKKβ、IKBα、NF-κB(P65)蛋白表达水平显著降低(P<0.05)。结论艾灸"天枢""上巨虚"改善IBS-D大鼠腹泻症状和内脏高敏感性可能与艾灸抑制海马与结肠组织IKKβ/IKBα/NF-κB信号通路有关,且其作用机制可能是通过脑-肠轴途径实现的。     

从富士康跳楼案看生产关系中的劳资矛盾及其法律问题

2010年以来,在短短不到半年的时间里,深圳富士康公司接连发生青年员工跳楼事件,造成10死2伤的严重后果。这不仅是富士康的悲剧,同时也反映了中国代工企业普遍存在的共性问题:缺乏健康的企业文化;政府监管迟到,执法不到位;企业党组织、工会"全方位"缺席等问题。但其本质却是劳资矛盾紧张、收入分配关系恶化的集中反映。我们需要通过完善企业管理,加强政府职能部门监管;改进企业管理,培养健康的企业文化;畅通劳工利益诉求途径,完善劳动立法等途径,构建起和谐的劳资关系。     

On Individual Business System in Normal Market

Individual business is a business entity system created in the practice of reform and opening up in China. It is the largest market entity in China, and its vitality lies in the "family culture" characteristics of Chinese society. Although individual businesses have been confirmed by law at all levels, they still have transitional and non-normative doubts. In some places, individual businesses have been transformed into enterprises. Individual businesses take advantage of the natural credit bond, low cost of organization and coordination, and are targeted to meet the needs of the market, which is conducive to absorbing employment and balancing wealth, easy to disperse and digest business risks, and has a competitive advantage in the low-end market. In a normal market, the rational choice of the parties concerned should be respected, the hierarchical structure of the market should be adapted, the complete ecology of market subjects should be maintained, and the reform of individual enterprises should be prudently implemented.     

Long-term Accompanying Service: Reflection on the Specialization in Social Work

Since 2006, China is undergoing a rapid growth in social work. In contrast with the rapid expansion of the scopes and fields for social work, the need for specialization in social work is creasing. Especially in mental health service in community, this demand is even higher. This article is intended to study a project on mental health in community called Assertive community treatment carried out in Shenzhen for 4 years, and finds that: a) the longstanding accompanying service is different from the short-term services; b) the longstanding accompanying service is another kind of specialized social work; c) the longstanding accompanying service is the best way for Chinese social workers to promote specialization of social work.     

电针时间对急性心肌缺血大鼠保护作用及心肌核转录因子- κB表达水平的影响

目的 观察电针时间对急性心肌缺血大鼠心脏保护作用及对心肌核转录因子-κB(nuclear transcrip-tic factor-κB,NF-κB)表达的影响,探讨电针干预急性心肌缺血的机制.方法 将SD大鼠随机分成正常组、模型组和电针即刻组、电针1d组、电针3d组、电针5d组、电针7d组,每组6只.采用结扎心脏冠状动脉左前降支法制备急性心肌缺血大鼠模型.电针心经"神门-通里"段,每次30 min,频率2 Hz、强度为1 mA的断续波分别进行即刻、1 d、3 d、5 d、7 d的电针治疗.用PowerLab 16导生理记录仪记录心电图;酶联免疫法检测血清肌酸激酶(creatine kinase,CK)、肌酸激酶同工酶(creatine kinase isoenzyme,CK-MB)、缺血修饰白蛋白(ischemia modified albumin,IMA)水平;Western blot检测心肌组织NF-κB蛋白表达水平,荧光定量PCR检测NF-κB mRNA表达水平.结果 与正常组比较,模型组大鼠ST段抬高,血清CK、CK-MB、IMA水平显著升高(P<0.05),NF-κB蛋白及mRNA表达水平显著升高(P<0.05).与模型组比较,即刻电针组大鼠血清IMA水平差异无统计学意义(P>0.05),CK、CK-MB水平显著降低(P<0.05),其他各电针组CK、CK-MB、IMA水平逐渐降低,以电针7 d组降低最为显著(P<0.05);即刻电针组、电针1 d组大鼠心肌组织中NF-κB蛋白及mRNA差异无统计学意义(P>0.05),电针1 d、3 d、5 d、7 d组表达水平呈下降趋势,以电针7 d组下降最为显著(P<0.05).结论 电针心经从第3天开始改善急性心肌缺血,且在治疗7 d后效果最为明显,其机制可能与逆转心肌组织NF-κB表达有关.     

Protection and Regulations to Status Property of Rich Second Generation

The status property of rich second generation is based on protection of status relations and private property rights, private property lateral expanse to the family property, and vertical expanse to inheritance property, but the current legal right of property has also shaped the power of the rich second generation. Policies for rich second generation should be based on the property nature of private right and sociality, the efficiency of property possession, the intergenerational equity to inheritance, and the abuse of property rights. Rich second generation have limited legitimacy to the property. It is necessary to improve the system of equity and trust to serve their property, and use the estate tax to achieve balance of interests between individual and society, but also need to overcome the fairness of procedure to constraint abuse of power.     

IL-33在小鼠皮肤创伤后损伤时间推断中的作用

目的通过观察白细胞介素-33(interleukin-33,IL-33)在皮肤创伤后的时序性变化规律,探讨IL-33在法医学实践中用于损伤时间推断的应用价值。方法利用直径为5mm的圆形锉刀在小鼠背部建造皮肤损伤模型,于伤后1h、3h、6h、12h、1d、3d、5d、7d、10d取损伤处组织,对照组在与创伤组小鼠相同部位取同等大小的皮肤样本。采用苏木精-伊红(hematoxylin-eosin,HE)染色法观察皮肤创伤后愈合过程中的形态学变化,通过Western印迹法、免疫组织化学染色和双重免疫荧光染色法检测皮肤创伤样本IL-33的表达变化。结果Western印迹法结果显示,伤后3h,IL-33蛋白表达稍有下降,6h后IL-33蛋白表达逐渐增加,于伤后3d达峰值,随后逐渐减少。免疫组织化学染色结果显示,在对照组皮肤的表皮、毛囊、皮脂腺及真皮中固有细胞有少量IL-33阳性表达,伤后3h,IL-33阳性细胞率开始增加,伤后3d达到峰值,随后逐渐减少。双重免疫荧光染色结果显示,伤后1~3d,IL-33阳性表达细胞主要为巨噬细胞,伤后5~7d,IL-33阳性表达细胞主要为肌成纤维细胞。HE染色结果显示该皮肤损伤模型创口愈合过程符合炎症的病理学发展规律。结论IL-33有望成为法医学推断皮肤损伤时间的参考指标。     

团体协作的企业文化建设

协作的企业文化并非是内部成员之间意见的一致,而是一种在将企业效益和个人利益有效结合前提下,去追求团体的最大力量,从而实现自身价值和企业目标。     

我国遗产税法律制度的设计

我国开征遗产税的必要性在于鼓励公民自食其力,调节社会贫富差距,补充现有的不完善税法。在已经基本具备了开征遗产税的条件下,我国必须从遗产税制、纳税人、征税范围、税率、起征点和征收管理等方面设计遗产税法律制度,同时为使开征遗产税顺利进行有必要建立个人财产登记、遗产评估及税前财产赠与等相关制度。     

H5亚型禽流感病毒核蛋白基因重组质粒的构建及其在Hela细胞的瞬时表达

采用PCR技术从重组质粒pMel-NP扩增得到了禽流感病毒(AIV)A/Goose/Guang-dong/1/96(H5N1)株的核蛋白(NP)基因,将其亚克隆至真核表达载体pVAX1上。将提取的pVAX-NP阳性克隆转染Hela细胞,48 h后经间接免疫荧光试验和Western-blotting检测,NP基因在Hela细胞中已成功瞬时表达。表达产物的分子质量约为57 ku,它与H5N1亚型AIV多克隆抗血清有较好的免疫反应。