鹿结核病流行株融合基因Rv3872-Rv3874-Rv3875的原核表达

以本实验室保存的鹿体内分离并鉴定的结核病流行株为模板,利用重叠延伸剪接技术设计融合基因的引物,扩增融合基因Rv3872-Rv3874-Rv3875,构建重组表达质粒pGEX-4T-1-Rv3872-Rv3874-Rv3875;重组表达质粒被转化入大肠杆菌BL21(DE3)后,IPTG诱导表达,并采用谷胱甘肽琼脂糖亲和层析方法对目的蛋白进行纯化。基因测序结果显示,融合基因Rv3872-Rv3874-Rv3875的序列与GenBank中序列的符合率为99.89%。SDS-PAGE分析显示,融合基因在大肠杆菌中成功被诱导表达,获得以可溶形式表达的融合蛋白,该蛋白的分子质量约为60ku。Western-blot鉴定结果显示,纯化好的融合蛋白能与鹿结核病的阳性血清发生反应,说明该融合蛋白具有良好的反应原性,这一结果为它在鹿结核病血清学诊断中的应用奠定了试验基础。     

Modern Scientific Evidence Pertaining to Criminal Investigations in the Chosun Dynasty Era (1392–1897 A.C.E.) in Korea

A guidebook detailing the process of forensic investigation was written in 1440 A.C.E. It outlines the fundamentals and details of each element of criminal investigation during the era of the Chosun dynasty in Korea. Because this old guidebook was written in terms of personal experience rather than on scientific basis, it includes many fallacies from the perspective of modern forensic science. However, the book describes methods to form a scientific basis for the experiments performed. We demonstrate the modern scientific basis for ancient methods to monitor trace amounts of blood and detect lethal arsenic poisoning from a postmortem examination as described in this old forensic guidebook. Traces of blood and arsenic poisoning were detected according to the respective color changes of brownish red, due to the reaction of ferric ions in blood with acetic ions of vinegar, and dark blue, due to the reaction of silver with arsenic sulfide.     

刑事诉讼法修改如何调整证据制度

证据概念以材料说代替事实说欠妥,可以考虑三种处理方式,倾向于删去关于证据定义的规定。证据分类应注意笔录证据规定的周延性,不应仅限于列举的几种,还应包含其他侦查行为笔录及审判笔录。证据排除规则,应当根据实践需要进一步明确排除范围,尤其对"等"字应作适当界定,防止过于宽泛无法把握。直接言辞证据规则,不仅需要规定证人出庭义务及保障措施,更应当限制书面证词的使用。就行政机关所取证据的司法运用,应当明确限制行政执法机关所取言词证据用于司法。有必要规定证据裁判原则;必须遵守质证原则,反对脱离庭审秘密核实证据。     

Monitoring of clobetasol propionate and betamethasone dipropionate as undeclared steroids in cosmetic products manufactured in Korea

Some cosmetic products manufactured in Korea have been suspected to contain anti-inflammatory corticosteroids, such as clobetasol propionate and betamethasone dipropionate, for the treatment of eczema, seborrhea and psoriasis, without any indication on the label of the cosmetic products. Due to their severe side effects, such as permanent skin atopy, these two corticosteroids in cosmetic products need to be monitored from a forensic point of view. Cosmetic product samples (number of samples=47) of manufacturers charged by consumers have been collected in local and online markets of Korea, and they were validated and analyzed by a simple high performance liquid chromatography (HPLC) method with ultraviolet diode array (UV-DAD). LC-MS/MS and LC-MS were used to confirm these steroids in cosmetic samples with diagnostic ions (m/z) and isotope ratio. Linearity was studied with 0.5-10μg/mL range in both steroids. Good correlation coefficients (r(2)≥0.999) were found, and their limits of quantifications were 0.59μg/mL and 0.66μg/mL in clobetasol propionate and betamethasone dipropionate, respectively. At three different concentrations spanning the linear dynamic ranges, mean recoveries were always higher than 93%, and precisions for intra-day and inter-day analyses were both less than 3.5%. The results show 32-96.4μg/g levels of clobetasol propionate in five different cosmetic products. Also, betamethasone dipropionate in a sample was monitored at the level of 195.1μg/g. This fact reveals that some manufacturers have added these steroids in their cosmetic products to advertise the treatment effect for skin atopy. Thus, these cosmetic products need to be monitored carefully, and ultimately removed from the market.     

犬组织中艾司唑仑在甲醛溶液中的稳定性

目的 研究甲醛溶液保存的生物样品中艾司唑仑的稳定性.方法 犬以艾司唑仑37.6mg/kg剂量灌胃,2h处死,解剖取其心、肝、肾、脑并分割为1g/份,分别放置于4%甲醛溶液中固定,用高效液相色谱法测定各组织及甲醛液中的艾司唑仑含量.结果 犬心、肝、肾、脑组织随着固定在甲醛溶液中时间的延长,组织及从组织溶解到甲醛溶液中的艾...     

温度对鸡早熟艾美球虫孢子囊形成的影响

从混合鸡球虫卵囊中分离单卵囊,感染增殖后收获大量单一种类球虫卵囊,将卵囊分别置于10~36℃、38~39℃、40℃恒温箱内培养,研究了温度对鸡早熟艾美球虫孢子囊形成的影响。结果显示,10~36℃时,孢子囊形成时间随温度提高而提前,每小时孢子囊形成率和增长幅度也随温度的提高而上升。10℃时,77 h初次形成孢子囊,孢子囊形成率达19.047%,经98 h孢子囊形成率达92.360%;36℃时,5 h初次形成孢子囊,孢子囊形成率达26.471%,经8 h孢子囊形成率达94.180%;38~39℃时,孢子囊形成率达一定时就不再上升,且随温度提高孢子囊形成率反而下降。38℃时,最高孢子囊形成率为52.800%,39℃时最高孢子囊形成率为12.500%;40℃时,球虫卵囊不会形成孢子囊,39~40℃是球虫卵囊形成孢子囊的临界温度。将卵囊分别置于40~65℃恒温干燥箱内经0.5 h处理后又经25~30℃培养24 h的结果表明,卵囊经40~50℃处理后仍可形成孢子囊,而经55℃以上温度处理就被杀死,45~50℃是球虫卵囊的生死临界温度。     

也论刑事一体化

本文以系统方法 ,对我国学界正在研究的“刑事一体化”进行了解剖与评析 ,指出 :要从系统性的角度对刑事法律及相关的规范、制度、政策在系统优化的过程中加以整合 ,从实体、程序、制度、政策的整体上把握刑法和相关规范的设计、运作 ,从系统性上规制刑事法体系 ;对刑事法的设计和操作要从整体结构的诸多关节点上细察、深究。     

新时代我国意识形态建设的五重维度北大核心SSCI

在新的历史方位下,应从五重维度澄明对我国意识形态建设的前提性、基础性认识。一是从实践维度定位意识形态建设的逻辑起点;二是从相对独立维度深化对意识形态建设的理论认知;三是从总体维度厘清意识形态建设的方法路径;四是从阶级维度明确意识形态建设的价值旨归;五是从批判维度解析意识形态建设的现实要求。从整体上系统把握这五重维度,对深刻明晰习近平关于意识形态工作的重要论述、保障我国意识形态建设的正确前进方向具有重要价值和意义。     

我国绿色证券投资基金绩效分析北大核心SSCI

当前,构建绿色金融体系已成为推动绿色经济快速发展、实现“碳达峰、碳中和”目标的重要途径。着眼绿色基金领域,通过分析基金重仓股明细,以及进行单因素评价模型的实证检验,探究我国绿色证券投资基金能否兼顾环境效益与经济效益的双重目标。结果显示,部分样本基金没能很好地执行投资策略,需调整资金配置,增加对绿色环保相关行业的投资;大部分样本基金在剔除掉风险因素的风险调整后收益的对比分析中,表现出了较强的获利能力,基金管理者市场调整和分散风险的能力较强。综合来看,我国绿色证券投资基金未能兼顾环境效益与经济效益的双重目标,环境效益有待进一步提高。为此,建议基金经理人对投资策略要充分予以执行,严格按照“绿色”标准选股投资;要进一步完善与绿色证券投资基金发展相关的配套机制和法律法规,丰富和完善绿色证券投资基金评价体系;提高投资者对绿色证券投资基金的认可度,建立多层次多元化投资主体结构。     

肾康注射液对肾小球系膜细胞自分泌ET-1、NO水平的影响

目的：探讨肾康注射液（ＳＫＩ）阻抑肾小球硬化（ＧＳ），防治慢性肾功能衰竭（ＣＲＦ）的部分作用机理，为ＳＫＩ临床应用提供依据。方法：采用放射免疫检测技术，观察ＳＫＩ对体外培养人肾小球系膜细胞（ＧＭＣ０自分泌内皮素－１（ＥＴ－１），一氧化氮（ＮＯ）水平的影响。结果：ＳＫＩ及其含药血清明显抑制自分泌ＥＴ－１，促进其自分泌ＮＯ，有量效依赖性；与肝素合用，协同抑制ＧＭＣ自分泌ＥＴ－１水平。