Immunohistochemical study of blood group activities in the alimentary canal in normal and pathologic conditions with reference to the nature of epithelial mucopolysaccharides

ABO(H)- and Lewis-activities in the human alimentary canal were investigated by the avidin-biotin-peroxidase complex method using commercial mouse monoclonal antibodies in order to clarify (1) the changes apparent in these activities in carcinoma of the digestive tract or premalignant intestinal metaplasia of the stomach and (2) the relationship of the activities to the histochemical properties of epithelial mucins examined by Alcian blue (pH 2.5)/periodic acid-Schiff (PAS) double staining. In intestinal metaplasia, ectopic goblet cells showed various degrees of ABO(H)-activities according to the PAS stainability of mucins and revealed Lea-activity even in a Le(a-b+) group. In carcinoma of the alimentary canal, ABO(H)- and Lewis-activities compatible with the donors' blood groups were located mainly at the PAS-positive cell surface, and in the Golgi bodies and secretions. It was thus concluded that quantitative and/or qualitative changes in blood group activities were closely associated with those of epithelial mucopolysaccharides.     

Japan's Policy for East Asia

Japanese foreign policy six decades after the end of World War II stands at a crossroads. The forces of globalization and the rise of the BRIC nations (Brazil, Russia, India, and China) seem to herald the end of the unipolar post-Cold War international system and the emergence of a new era defined by multi-polarity and multilateralism. Such global trends are manifest most clearly in Japan's own backyard, where phenomenal region-wide economic growth, a gradual redistribution of power (in particular the rise of China and India), and the increasingly important role of multilateral cooperation and regional institutions are dramatically transforming East Asia. This trend shows no signs of slowing down, much less reversing itself; nor would it be in Japan's interest to pursue any policy that seeks to do so. Nevertheless, the transformation itself remains almost on auto-pilot, lacking a clear long-term guiding vision. After presenting a brief overview of Japan's past policy toward the region, the goal of this paper will be to articulate such a vision through a series of policy proposals through which to ensure future peace, stability, and prosperity in East Asia.     

A new triplex STR system without irregular alleles by silver staining and its potential application to forensic analysis

In order to increase the discriminating power of DNA analysis in forensic science, we devised a new triplex STR system using three novel STR loci we previously reported, D14S299 (wglc5), D15S233 (wgldl), and 9q2h2. We designated this system a CDH triplex system. The CDH triplex system showed a high discriminating power, especially in Caucasians. This system is composed of three STR loci showing only regular tetranucleotide repeat alleles. We easily enlarged the databases mainly of Japanese, using this system, and compared them with those of Caucasian and Chinese. This CDH triplex system therefore appears to be useful for forensic practice.     

In pursuit of universal values

Is universality inherent in freedom and human rights? Yes, says Keijiro Tanaka, trustee and senior advisor at IIPS. Only democratic systems of government embodying constitutional liberalism can safeguard the freedoms and rights of their citizens, and the spread of democracy offers the best chance for ensuring a peaceful world. However, freedoms and rights must go hand in hand with duties and responsibilities, and Tanaka argues for codifying these in a document which would supplement and support the Universal Declaration of Human Rights. He says “Asian values” as championed by some Asian leaders do not have the same universal quality as the rights and freedoms set forth in the Universal Declaration of Human Rights, because they are traditional moral precepts or social virtues that can be used for both good and evil. He feels Japan should be more responsible in the pursuit of universal values which are shared by all other liberal democracies.     

Egocentric bias in perceived fairness: Is it observed in Japan?

Experiment 1 was a Japanese replication of the studies reported by Messicket al. (1985) and Liebrandet al. (1986). Subjects were asked to write down fair or unfair behaviors that they or others did, giving as many examples as possible in 5 min. As in the previous studies, the subjects began more fair behaviors with the word I than with others. Likewise, they began more unfair behaviors with the word others than with I. In Experiment 2, 80 examples of behaviors (40 fair and 40 unfair) were selected randomly from the results of Experiment 1. The difference between the number of frequent behaviors sorted into the fair/I category and that sorted into the unfair/I category was greater than that between the number of behaviors sorted into the fair/others and that sorted to be unfair/others. Salient behaviors were more likely to be sorted into the category of unfair than into fair, and more likely to be sorted into others than I. On the whole, the egocentric bias of fairness was confirmed in Japan as well as in the Netherlands and the United States. However, in both experiments, gender differences were found; women, compared to men, recalled more others' behavior than their own and were likely to attribute fair and/or unfair behavior to others rather than to themselves.     

Change in the Spatial Dimensions of Party Conflict: The Case of Japan in the 1990s

The political landscape of Japan changed drastically in the early 1990s with new parties forming, the main government party losing power temporarily, and the traditional rival parties forming a coalition government. This article examines how the spatial dimensions of party conflict changed according to surveys of the mass public. Contrary to theoretical expectations based on the stabilizing effects of party identification, we find that the structure of public attitudes toward the parties changed considerably as the party system changed. Abrupt changes in the party system were reflected in changed cleavage patterns. Independence increased during this period and changed from being perceived as a separate dimension to being seen as part of an anti-politics-as-usual dimension.     

Shedding Light on the Shadow Economy: A Nighttime Light Approach

Measuring the shadow economy is inherently difficult, but critical for understanding economic development. Using census data on formal and informal sectors in Cambodia, we document that 96.6 per cent of non-farm establishments do not formally register with the government, and their sales accounted for 76.6 per cent of total sales in 2011. Estimating their past sales from changes in nighttime light for 1993–2010, we find that both formal and informal firms increased their estimated sales. The share of informal sales increased from 68.8 per cent in 1993 to 76.6 per cent in 2011, suggesting that the informal sector increased quantitatively in both absolute and relative terms over time.     

Monoclonal antibody against tetrahydrocannabinolic acid distinguishes Cannabis sativa samples from different plant species

The cross-reaction of anti-delta 1-THCA MAb against other cannabinoids was very wide. However, other naturally occurring and synthetic phenolics including opium alkaloids did not react to the MAb. Using this ELISA, this paper reports application of the competitive ELISA for detection of marijuana samples. The ELISA described here was very sensitive to the ether extracts of marijuana samples when compared to those of other plants. The assay provided a sensitive method useful for the judge of marijuana samples.     

Solid-phase extraction and analysis of 20 antidepressant drugs in human plasma by LC/MS with SSI method

A simultaneous determination of 20 antidepressant drugs (imipramine, amitriptyline, desipramine, trimipramine, nortriptyline, clomipramine, amoxapine, lofepramine, dosulepin, maprotiline, mianserin, setiptiline, trazodone, fluvoxamine, paroxetine, milnacipran, sulpiride, tandspirone, methylphenidate and melitracen) in human plasma was developed using LC/MS with sonic spray ionization (SSI) method. These drugs showed good separation and sensitivity by LC-MS using an Inertsil C-8 column with methanol:10mM ammonium acetate (pH 5.0):acetonitrile (70:20:10) as mobile phase at 0.10 mL/min at 35 degrees C. Solid-phase extraction of these drugs added to the human plasma was performed with an Oasis HLB cartridge column. Recovery and limit of detection of these drugs were between 69 and 102% and between 0.03 and 0.63 microg/mL, respectively. The present procedure offers an easier and more convenient screening method for antidepressants, and will be useful for forensic toxicology investigations.