Fentanyl Detection Using Eosin Y Paper Assays,

Eosin Y is a potential new color test for use in detecting illicit drugs that has not been extensively studied. In the present study, a variety of drugs of abuse and fentanyl analogues were tested to determine which drugs will bind to eosin Y, which functional groups are capable of binding and eliciting a color change, and a mechanism for eosin Y binding to fentanyl. Further, these agents were combined with common cutting agents and other drugs of abuse in order to determine the fentanyl detection limit in a drug mixture using an eosin Y test strip. Additionally, cobalt thiocyanate was used to determine whether the combination of cobalt thiocyanate and eosin Y has the potential to identify fentanyl. Through the testing performed, we concluded that (i) Eosin Y is capable of detecting low amounts of fentanyl down to 1%, (ii) Eosin Y binds to select tertiary amines to produce an orange to pink color change, and (iii) Eosin Y binds to the nonpiperidine ring nitrogen of fentanyl as a primary binding site and the piperidine ring nitrogen as a secondary binding site. While the cobalt thiocyanate assay detected 1% fentanyl in some of the mixtures, eosin Y detected 1% fentanyl in all mixtures. Finally, eosin Y was able to detect fentanyl in forensic case samples containing heroin and various cutting agents. Based on our results, eosin Y has the potential to screen for fentanyl and fentanyl analogues and can detect fentanyl in low amounts when mixed with common cutting agents.     

DNA short tandem repeat profiling of three North Indian populations.

Population: Fifty healthy unrelated individuals were randomly chosen from each of the three populations viz., Bhargavas, Chaturvedies, and Brahmins. Three generation pedigree charts were prepared to ensure sirname endogamy in Bhargavas Chaturvedies and group endogamy in Brahmins subjects were chosen from several parts of Uttar Pradesh, a northern state of the Indian republic.     

Effect of various factors on the activity of sucrase from the larvae of Sesamia inferens

Salivary gland sucrase activity fromSesamia inferens Wlk. was optimal at pH 5.8 and temperature 35°. It increased up to 1.5% final sucrose concentration, but higher concentrations had no effect. A dialysed preparation showed a 37.5% reduction in the activity. It was accelerated by tryptophan and inhibited by all other amino acids used. Gut sucrase activity ofS. inferens was optimal at pH 6.2 and temperature 30°. It increased sharply up to 0.66% final sucrose concentration, and then gradually up to 1.5% while further increase in the concentration had no effect. A dialysed preparation showed an 8% increase in activity. Activity was not affected by serine; was accelerated by arginine, cystine, methionine, tryptophan and valine; and inhibited by alanine, glutamine, glycine, hydroxyproline, leucine, phenylalanine, proline and tyrosine. End-products increased with the incubation period and showed inhibitory effects. With the increase of enzyme concentration the rate of sucrose hydrolysis also increased. K⁺ ions accelerated the activity of the dialysed preparation. It was also accelerated by Li⁺⁺ ions up to their final concentration of 0.001 M, but inhibited when their final concentration increased (0.01 M and 0.025 M). TRIS also inhibited the activity.

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Résumé Deux types d'extraits ont été étudiés séparément: la sucrase extraite des glandes salivaires et celle extraite de l'intestin moyen. L'activité optimale de la sucrase salivaire s'observe pour un pH 5,8 et une température de 35°; celle de la sucrase de l'intestin moyen pour un pH 6,2 et une température de 30°. Cependant les extraits dialysés, d' origine salivaire ou d'origine intestinale, ont une activité optimum pour un même pH (5,8). L'activité des deux sucrases s'accro?t proportionnellement à la durée de la période d'incubation. L'activité de la sucrase salivaire s'accro?t nettement, jusqu'à une concentration finale en sucrose de 1.5%, alors que pour la sucrase intestinale, 90% de l'activité maximale est réalisée pour une concentration finale en sucrose de 0.66%. Quand la concentration en sucrose atteint ou dépasse 1,5% il se forme alors un oligosaccharide dont la synthèse équilibre l'hydrolyse du sucrose, ce qui rend l'activité de la sucrase constante au-dessus d'une concentration finale en sucrose≥1,5%. Cette synthèse d'un oligosaccharide intervient quand on utilise une forte concentration de sucrase, ce qui entraine la production de glucose et fructose en grande quantité; avec une faible concentration de l'extrait on n'observe pas la synthèse d'oligosaccharide. Les ions K⁺ accélèrent l'activité des extraits dialysés, d'origine salivarie ou intestinale; les ions Li⁺⁺ sont également favorables jusqu'à une concentration de 0,001 M, cette action devenant inhibitrice à partir de 0,005 ou 0.025 M; les ions TRIS, à 0,025 M ont également un effet inhibiteur. Certains acides aminés accélèrent l'activité de la sucrase salivaire ou intestinale, d'autres au contraire ont une action inhibitrice.

     

Tractors,tubewells and cropping intensity in the Indian Punjab

This paper quantifies the cropping intensity effects of tractors and tubewells in the Indian Punjab. In addition to the conventional measure of cropping intensity, a new index, which takes account of the time duration of crops grown, is specified. The advantage of tractor ownership vs. hiring the machine is also assessed as are the effects of farm size and agro‐climatic zones. Relative to bullocks and canal irrigation, tractors and tubewells are respectively found to be associated with higher cropping intensities, as measured by both indices. Owned tractors have an advantage over hired ones and the effect of tubewells is substantially greater than of tractors.     

Consolidation of Indian PSU banks and the way forward

The increasing non‐performing assets (NPAs) and to meet the higher funding needs, India witnessed the biggest consolidation in public sector banks. In India the surging NPAs is long witnessed concern and major economic reforms were initiated in Indian banking to curb the issue. The recent merger has brought in a major policy concern with this consolidation which is availability of loans to smaller businesses. The paper analyses the impact of banks consolidation on Indian economy by considering both positive as well as negative aspects of banks mergers. The paper also presents the history of Indian banking and recommending a way forward.     

Genetically-induced variability of alcohol metabolism and its effect on drinking behavior and predisposition to alcoholism

Alcoholism is one of the most challenging current health problems in the Western countries with far-reaching medical, social, and economic consequences. There are a series of factors that interact in predisposing or protecting an individual against alcoholism and alcohol-related disorders. This article surveys the state of our knowledge concerning the biochemical and genetic variations in alcohol metabolism and their implications in alcohol sensitivity, alcohol drinking habits, and alcoholism in different racial/ethnic groups. The major pathway for the degradation of ethanol is its oxidation to hydrogen and acetaldehyde--to which many of the toxic effects of ethanol can be attributed. Variations in alcohol and acetaldehyde metabolism via genetically determined polymorphisms in alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) seem to play an important role in individual and racial differences in acute and chronic reactions to alcohol, alcohol drinking habits, as well as vulnerability to organ damage after chronic alcohol abuse. Alcohol sensitivity and associated discomfort symptoms accompanying alcohol ingestion may be determinental for the significantly low incidence of alcoholism among the Japanese, Chinese and other Orientals of Mongoloid origin. An abnormal ALDH isozyme has been found to be widely prevalent among individuals of the Mongoloid race and is mainly responsible for the acute sensitivity to alcohol commonly observed in this race. Persons sensitive to alcohol by virtue of their genetically controlled ALDH isozyme deficiency may be discouraged from drinking large amounts of alcohol in their daily life due to the initial adverse reaction experienced after drinking alcohol. Indeed, a significantly low incidence of the mitochondrial ALDH isozyme deficiency has been observed in alcoholics as compared to psychiatric patients, drug dependents and healthy controls in Japan. How far any variation in ADH and/or ALDH activity among individuals of Caucasian origin will have similar effects has yet to be studied.     

E-Governance to Cut Corruption in Public Service Delivery: A Case Study of Fiji

Experience in a number of developed and developing countries has shown that E-Governance initiatives can promote greater transparency with the goal of cutting corruption. This article examines perceptions of public service delivery in Fiji to explore the potential of E-Governance to cut corruption and improve governance. Based on a survey of community perceptions and a review of selected literature it suggests that service-delivery oriented IT initiatives can contribute to an effective, multi-pronged strategy to cut corruption in the Fiji public sector.