Highly reliable quantification of proteins such as members of the HSP70 superfamily based on the grey scale index via immune detection stained bands on a Western blot

The HSP70 superfamily is a reliable biomarker for hyperthermia, hypothermia and hypoxia. The Enzyme-linked Immunosorbent Assay (ELISA) respectively immunohistochemically staining methods are the typically used techniques for the quantification of those proteins. As the costs for reagents and devices as well as the work schedule of these methods are immense it was the goal of our study to develop an easy and reliable method to quantify the concentration of specific proteins. We established a procedure to measure the relative concentration of proteins fixed on ROTI(?) PVDF membranes via Western blot, calculating the relative protein concentration in dependency to the grey scale index of the normalized and digitalized pictures of the bands on the blots.     

Law and Firms' Access to Finance

This article examines how a country’s legal origin influencesthe operation of its financial system by using firm-level surveydata across a broad cross-section of countries on the obstaclesthat firms face in raising external finance. Using panel regressions,the article assesses two channels through which legal originmay influence the financial system. We find that the adaptabilityof a country’s legal system is more important for explainingthe obstacles that firms face in accessing external financethan the political independence of the judiciary.     

Sequencing of mitochondrial DNA and the problem of human specificity

When analysing trace materials and degraded DNA the issue of human specificity is highly important. Especially when it comes down to the analysis of mitochondrial DNA which is extremely susceptible to contamination authenticity is the main question. Therefore in the presented study mitochondrial primers were tested on their human specificity. In all cases it was possible to amplify DNA of animals with human mt-primers. These unintentional amplifications could only be decreased by choosing austere PCR parameters. The study implies the importance of comprehensive evaluation of primers, chemicals and PCR parameters.     

Accidental death of disoriented persons in long term care facilities

Five cases of accidental death occurring in connection with physical restraints in long-term care facilities are reported. The position in which the deceased were found, autopsy findings, police investigations as well as the evaluation of each case under criminal law are described. The investigations by the prosecution as well as the attention of the public media have meanwhile contributed to an improvement of the technical standards and a reduction of the risk in applying such restraints under the Law on Medical Devices.     

Determination of ethyl glucuronide in human hair by SPE and LC-MS/MS

A method for the sensitive and selective determination of ethyl glucuronide (EtG) in hair has been developed using solid-phase extraction (SPE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Washed and cut hair segments were extracted by ultrasonication (3h, 50 degrees C) and the extracts were cleaned-up with aminopropyl SPE columns. LC-MS/MS analysis was performed using a polar-endcapped phenyl-hexyl-RP-phase with negative mode electrospray ionisation (ESI) using a triple quadrupole mass spectrometer (Sciex API 365) with a turboionspray source and post-column addition of acetonitrile for enhanced sensitivity. The MS/MS transitions monitored were m/z 221 -->75 for EtG and 226 -->75 for D(5)-EtG as an internal standard. The method was selective and sensitive, with a detection limit of 51 pg/mg hair at a signal-to-noise ratio of 3:1. The mean recovery was 96%, with an intra- and inter-day precision of less than 11.7% at a concentration of 200 pg/mg. The linearity was assessed in the range of 25-2000 pg/mg hair, with a correlation coefficient of 0.997. The method was successfully applied to 97 human hair samples which were taken at autopsies from persons with known alcoholism or were obtained from alcoholics who were hospitalized for ethanol withdrawal, from social drinkers and from children having not consumed any alcohol. Although, approximately two-third of the alcoholics showed EtG concentrations in hair of higher than 51 pg/mg (up to >4000 pg/mg), in one-third the EtG concentration was below the detection limit. However, only in one of five hair samples of "social drinkers", the EtG concentration was above the detection limit (51 pg/mg). No EtG has been detected in the hair of children. These investigations demonstrate that heavy alcohol consumption may be but not necessarily has to be detectable by EtG analysis in hair.     

Bewertungskonstellationen. Theoretische Überlegungen zur Soziologie der Bewertung

The article discusses central research questions currently prevailing in the sociology of valuation. It argues that the perspective of “methodological situationalism”, which currently dominates within valuation studies, overemphasizes “moments” of valuation, and thus ignores trans-situational forces, which are highly consequential in processes of valuation. On the basis of this diagnosis, the article puts forth the concept of “valuation constellations” which allows observing valuations’ transsituativity in a systematical manner. The concept distinguishes different positions – valuee, valuator, and audience – and emphasizes the relevance of their relations in shaping valuations. Moreover, the article underscores that valuation constellations always unfold against the background of trans-situationally valid rules as well as technological infrastructures operating on a trans-situational level. Ultimately, the concept facilitates new research questions for the sociology of valuation.     

Introduction: Authority Building in International Administered Territories

This introduction to the special section on “Authority Building in International Administered Territories” begins by summarizing the relevant literature on international administrations. To conceptualize authority building as an ongoing process in which international and local actors confirm – or refuse – authority claims, secondly, two types of authority are differentiated: ‘interpretative authority’ and ‘performing authority’. This sociological conceptualization of authority building allows, thirdly, a discussion on the restrictions and challenges that international administrations face in their attempts to establish authority on the ground. Finally, an overview of the section’s articles is provided.     

The Impact of Online versus Offline Campaign Information on Citizens’ Knowledge,Attitudes and Political Behaviour: Comparing the German Federal Elections of 2005 and 2009

Against the background of the debate about how the internet changes democratic processes, this article assesses the impact of online versus offline campaign information on citizens’ knowledge, attitudes and political behaviour using data from two pre-/post-election surveys conducted at the German federal elections in 2005 and 2009. Our results show that sophisticated and less sophisticated citizens alike gain knowledge when using online information. Moreover, we find that people exposed to online information develop less ambivalent attitudes towards candidates and parties but do not take on more extreme issue positions. Online information is ineffective in stimulating turnout but compared to traditional news media, the internet gives smaller parties the chance to gain votes. We conclude that overall online information can enhance election campaigns. However, the amount of change should not be overstated as the effects are similar to those of offline political information and depend on the campaign context.