Social perceptions on genomics in four Latin American countries. Ethical-legal implications]

The authors analyze under an ethical and legal perspective the consequences and anxieties generated by the human genome project in the population of four Latin American countries: Argentine, Chile, México and Perú, through bibliographical analysis and interviews done to biomedical researches, lawyers and legislators, students and lay civilians.     

Risk Analysis of Nanomaterials: Exposing Nanotechnology's Naked Emperor

Risk analysis (encompassing risk assessment, management, and communication) is touted internationally as the most appropriate approach for governing nanomaterials. In this article, we survey existing criticisms of risk assessment as a basis for regulatory decision making on emerging technologies, particularly highlighting its exclusion of key societal dimensions, its epistemological underdetermination, and its lack of democratic accountability. We then review the specific case of nanomaterials and identify six major barriers to the effective operation of both risk assessment and risk management. These include a lack of: nano‐specific regulatory requirements, shared definitions, validated and accessible methods for safety testing, available scientific knowledge, reliable information on commercial use, and capacity for exposure mitigation. Finding the knowledge, standards, methods, tools, definitions, capacity, and political commitment all insufficient, we argue that risk analysis is a “naked emperor” for nanomaterial governance. We therefore suggest that additional concepts and approaches are essential for nanomaterials policy and regulation.     

Legislative genesis and judicial death of a directive: The European Court of Justice invalidated the data retention directive (2006/24/EC) thereby creating a sustained period of legal uncertainty about the validity of national laws which enacted it

The Grand Chamber has ruled that the data retention directive was invalid ex tunc since it seriously interfered with the fundamental rights to respect for private life and protection of personal data and exceeded the limits of the principle of proportionality which are provided for in the Charter. The scope and temporal effects of this ruling should be clarified, especially its legal impacts on national laws of Member States which enacted the directive. In addition, the findings of the Grand Chamber on geographical safeguards have far-reaching implications on the retention and storage of personal data in the EU.     

Real-time PCR designs to estimate nuclear and mitochondrial DNA copy number in forensic and ancient DNA studies

We explore different designs to estimate both nuclear and mitochondrial human DNA (mtDNA) content based on the detection of the 5' nuclease activity of the Taq DNA polymerase using fluorogenic probes and a real-time quantitative PCR detection system. Human mtDNA quantification was accomplished by monitoring the real-time progress of the PCR-amplification of two different fragment sizes (113 and 287 bp) within the hypervariable region I (HV1) of the mtDNA control region, using two fluorogenic probes to specifically determine the mtDNA copy of each fragment size category. This mtDNA real-time PCR design has been used to assess the mtDNA preservation (copy number and degradation state) of DNA samples retrieved from 500 to 1500 years old human remains that showed low copy number and highly degraded mtDNA. The quantification of nuclear DNA was achieved by real-time PCR of a segment of the X-Y homologous amelogenin (AMG) gene that allowed the simultaneous estimation of a Y-specific fragment (AMGY: 112 bp) and a X-specific fragment (AMGX: 106 bp) making possible not only haploid or diploid DNA quantitation but also sex determination. The AMG real-time PCR design has been used to quantify a set of 57 DNA samples from 4-5 years old forensic bone remains with improved sensitivity compared with the slot-blot hybridization method. The potential utility of this technology to improve the quality of some PCR-based forensic and ancient DNA studies (microsatellite typing and mtDNA sequencing) is discussed.     

Validation of the Attitudes About Aggression in Dating Situations (AADS) and the Justification of Verbal/Coercive Tactics Scale (JVCT) in Spanish Adolescents

The aim of this study was to analyze the psychometric properties of two instruments that assess attitudes justifying use of aggression in adolescents?? dating relationships: the Attitudes About Aggression in Dating Situations (AADS) and the Justification of Verbal/Coercive Tactics Scale (JVCT). The sample was made up of 2,856 adolescents from different high schools of Madrid. Results supported the three-factor structure for the AADS and the six-factor structure for the JVCT, which was consistent with the theory and with previous research. As expected, the relation between attitudes and aggressive behavior was between small and moderate but significant in most cases. Furthermore, attitudes differed depending on the type of violence, the respondent??s sex, and on whether aggression is carried out by a man or a woman. Finally, the potential applications of these two scales are discussed, and it is concluded that both constitute useful tools to assess justification of aggression in dating relationships in young couples.     

Using the institutional grammar tool to understand regulatory compliance: The case of Colorado aquaculture

What is the relationship between the design of regulations and levels of individual compliance? To answer this question, Crawford and Ostrom's institutional grammar tool is used to deconstruct regulations governing the aquaculture industry in Colorado, USA. Compliance with the deconstructed regulatory components is then assessed based on the perceptions of the appropriateness of the regulations, involvement in designing the regulations, and intrinsic and extrinsic motivations. The findings suggest that levels of compliance with regulations vary across and within individuals regarding various aspects of the regulatory components. As expected, the level of compliance is affected by the perceived appropriateness of regulations, participation in designing the regulations, and feelings of guilt and fear of social disapproval. Furthermore, there is a strong degree of interdependence among the written components, as identified by the institutional grammar tool, in affecting compliance levels. The paper contributes to the regulation and compliance literature by illustrating the utility of the institutional grammar tool in understanding regulatory content, applying a new Q‐Sort technique for measuring individual levels of compliance, and providing a rare exploration into feelings of guilt and fear outside of the laboratory setting.     

Age estimation through histological study of trabecular volume and cortical bone width of the iliac crest

There have been many methods proposed to estimate the age of human bones in forensic medicine or in forensic anthropology, including those that assess histological structures. In this study, age was evaluated through the histomorphometric study of trabecular volume and cortical width of the right iliac crest. A total of 25 samples A total of 66 samples (42 male and 24 female) were obtained from necropsies of judicial cases, with ages ranging from 13 to 58years, who had died a sudden or violent death. The samples were obtained using Bordier's trocar for bone biopsy, in a period not exceeding 24h after death. The samples were examined without decalcification, with 96° alcohol dehydration, embedding in methyl methacrylate, and sectioned with a microtome Reichert (3μm). The sections were fixed on microscope slides and stained using toluidine blue, Goldner trichrome and hematoxylin-eosin methods. The samples were studied under a Leica DM LB microscope and the images were digitally captured. The digital images were processed using an image analysis program and data were analyzed using the SPSS statistical package. The results demonstrated that the combination of the trabecular volume and cortical width acted as predictors of the age in subjects which did not suffer from any deterioration of bone and/or mineral metabolism.     

The Association between Maternal Exposure to Intimate Partner Violence and Emotional and Behavioral Problems in Spanish Children and Adolescents

This correlational cross-sectional study was designed to investigate whether the intimate partner violence (IPV) suffered by mothers (physical and psychological maltreatment), the neglect suffered by children, and the maltreatment (physical and psychological) directly suffered by children are statistically associated with an increase in the probability of the child’s suffering psychopathological problems. The sample consisted of 189 Spanish children aged 6 to 17 and their mothers, recruited from Centers of Specialized Assistance for Women Victims of IPV. The results of a canonical correlation analysis showed that the most significant problems suffered by the children were both externalizing and internalizing ones. In girls, the maltreatment suffered by their mothers was directly related to a larger frequency of somatic complaints than in boys. In addition, physical maltreatment to the mother and emotional maltreatment suffered by the child exhibited a statistically significant relationship with aggressive behaviour, thought problems, rule-breaking behaviour, attention problems, and withdrawn-depressed.     

A NOT SO MODEST PROPOSAL TO HUMANIZE THE LEGAL PROFESSION

This article first appeared in the Los Angeles County Bar Association's Family Law Section quarterly , News and Review. In a very personal and insightful way, the author examines the change which women have brought to the practice of family law .