Genetic and chemical components analysis of Papaver setigerum naturalized in Korea

Of the 110 species of genus Papaver, only Papaver somniferum and P. setigerum are controlled poppies in Korea. All poppy samples share similar morphology therefore it is important to check if they contain controlled substances such as morphine and codeine for forensic purpose. Since the alkaloid content of Papaver plants varies according to their growing stage, chemical components analysis alone is not enough to identify exact species. In 2010, hundreds of poppy plants suspected to be P. somniferum were found in Jeju Island, South Korea. They had a slightly different but overall similar appearance to P. somniferum. Using GC-MS analysis, codeine, rhoeadine, papaverine, protopine, noscapine, setigeridine and trace amounts of morphine were detected in these samples. Although their chemical components were different from what has been described in literatures for P. setigerum, they could be assumed to be P. setigerum based on their morphological features and GC-MS results. Also, chromosome numbers using their seeds showed 2n=44 and the numbers were in accordance with those of P. setigerum. Nucleotide substitution or insertion/deletion of ITS (internal transcribed spacer), 18S rRNA (ribosomal RNA), rbcL (large subunit of ribulose 1,5-bisphosphate carboxylase), trnL-trnF IGS (intergenic spacer), trnL intron and psbA-trnH were assessed as universal genetic markers for P. setigerum. Also, genetic analysis using six target genes involved in the biosynthesis of benzylisoquinoline alkaloids, including TYDC (tyrosine/dopa decarboxylase), SAT (salutaridinol-7-O-acetyltransferase), BBE (berberine bridge enzyme), COR (codeinone reductase), CYP80B1 ((S)-N-methylcoclaurine 3'-hydroxylase) and NCS (norcoclaurine synthase) were tested as Papaver-specific genetic markers by the existence of their PCR products. From the results, the sequences of the 6 universal genetic markers and 6 Papaver-specific genetic markers for P. setigerum were identified and then Genbank accession numbers of them were registered in NCBI. Also, the trnL intron and psbA-trnH nucleic acid sequences of the 7 Papaver species were identified and registered.     

Does Self-Control Influence Maternal Attachment? A Reciprocal Effects Analysis from Early Childhood Through Middle Adolescence

Objectives

The purpose of this study is twofold. First, this study assesses the extent to which self-control and maternal attachment mutually influence one another. Second, it investigates whether this process continues to occur during adolescence. To date, studies of the etiology of self-control have yet to adequately address these issues, despite the fact that a number of theoretical perspectives emphasize the reciprocal nature of the parent-child relationship.

Methods

The current study seeks to shed light on these issues by examining the relationship between self-control and maternal attachment using structural equation modeling for eight waves of data spanning a period of time that encompasses early childhood through middle adolescence.

Results

The results yield two findings bearing on the adequacy of Gottfredson and Hirschi??s model of self-control development. First, measures of self-control and maternal attachment were found to mutually influence one another during childhood. Second, these effects were reduced to nonsignificance during adolescence.

Conclusions

This study finds that self-control emerges during childhood in a complex manner in which it both shapes and is shaped by parental attachment.     

State Capacity and Terrorism: A Two-Dimensional Approach

Conventional wisdom suggests that dissident groups use terrorism when they face an overwhelmingly more powerful state, yet attacks in developing countries have predominated in the post-Cold War era, suggesting that terrorism is an increasingly weak state phenomenon. Cross-national studies of terrorism find mixed results for how common measures of state capacity influence terrorism. We argue that these indeterminate findings are due in part to a partial understanding of both what constitutes state capacity and how different aspects of state strength or weakness relate to the propensity of groups to use terrorism. We decompose state capacity into two dimensions that we theorize are particularly relevant to dissident groups: military capacity, or the ability to project conventional military force, and bureaucratic/administrative capacity. Our analysis supports the claim that terrorist attacks are more frequently targeted at states with large, technologically sophisticated militaries but less frequently targeted at states with higher bureaucratic and administrative capacity. We also compare two militarily capable states, France and Russia, that have had different recent experiences with terrorism to help illustrate the causal mechanisms involved. Evidence from our models and cases suggest that states can be capable in different ways, and these various capabilities create differing incentives for using terror as a strategic and tactical tool.     

Implementation of Digital-Era Governance: The Case of Open Data in U.S. Cities

This article examines the institutional factors that influence the implementation of open data platforms in U.S. cities. Public management scholarship has argued that governance can be transformed by new information technologies that improve transparency and engagement, reduce administrative costs, and support performance management systems. However, this argument ignores key risks for administrators, as well as institutional and political obstacles that can thwart implementation. This article uses hierarchical negative binomial regression to analyze the organizational and institutional features influencing implementation in more than 1,500 departments across 60 cities. Department type and administrative capacity are strongly associated with the number of open data files available, while city-level institutional characteristics and administrative capacity are not significant factors. Municipal demographics are also identified as a factor, suggesting a potential demand-side influence from wealthy and technologically proficient residents. Evidence for Practice

• The implementation of open data policies benefits from targeted approaches at the department level rather than uniform, citywide objectives or requirements.
• City executive-level positions such as chief data or information officers are not necessarily associated with successful implementation, measured by the number of open data files made available.
• Open data implementation involves additional administrative responsibilities and labor at the department level, so city administrators looking to expand the number and variety of data sets available through their open data platforms should devote time and resources to working directly with departments to facilitate and encourage data sharing.
• Administrators looking to expand the number and variety of data sets available through their open data platforms should consider the costs associated with investing in increasing individual departments’ abilities to balance the additional administrative responsibilities and labor involved.

     

Tarasoff in Missouri: The jurisprudence of a mental health provider's duty to warn and protect non-patients of potential risks from patients

In 1976, the Supreme Court of California issued its well-known Tarasoff Principle. From this principle, other courts found a duty to warn, and some found more than just a duty to warn, a duty to protect. As courts in other states adopted a version of the Tarasoff Principle, they issued a wide variety of third-party liability rules. In light of the dynamic, everchanging Tarasoff jurisprudence in the United States and recent relevant appellate court opinion in Missouri, a timely updated summary and update of Tarasoff-related jurisprudence in Missouri is warranted. In the present analysis, we compiled the four appellate court decisions that pertained to the questions of Tarasoff-like third-party liability in the State of Missouri: Sherrill v. Wilson (1983), Matt v. Burrell (1995), Bradley v. Ray (1995), and Virgin v. Hopewell (2001). We reviewed all legal measures for clinicians to protect nonpatients in Missouri, not just those that relate to protecting nonpatients from violence as in a Tarasof-like scenario. Thus, this paper concisely provides a compendium of such options and allows for a meaningful comparison of which legal, protective measures are mandatory and which are permissive, thereby evoking the question of whether measures of protecting nonpatients from a patient's violent acts ought to be mandatory duties or permissive application of professional judgment.     

关于将松北新区建成高能级发展科技创新城的调研报告

盖如垠书记到任哈尔滨后,提出了“北跃、南拓、中兴、强县”的发展战略构想,并经市委十二届七次全会丰富完善已成为哈尔滨未来发展的战略取向。通过学习市委全会精神,我们认为,这一战略构想符合哈尔滨的发展实际,十分及时地抓住了哈尔滨发展的关键和主轴,是哈尔滨经济社会实现跨越式发展的重要抉择。近期,我们班的全体学员对实施“北跃”战略——将松北新区打造成具有高能级发展功能的科技创新城问题进行了调研和思考,形成了此报告,供市委决策参考。     

Carbon‐Based Fingerprint Powder as a One‐Step Development and Matrix Application for High‐Resolution Mass Spectrometry Imaging of Latent Fingerprints

Carbon‐based materials are often used as matrices for matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) and its imaging (MALDI‐MSI). However, researchers have refrained from using carbon‐based fingerprint powder (CFP) as a matrix due to high background and contamination. In this work, the compatibility of CFP is reevaluated with MALDI‐MSI using a high‐resolution mass spectrometer (HRMS) and compared to traditional organic matrices. Relevant fingerprint compounds were easily distinguished from carbon cluster peaks when using HRMS. For fair comparison, half of a fingerprint was dusted with CFP while the other half was dusted with traditional organic matrices. All compounds studied had comparable, or higher, signal‐to‐noise (S/N) ratios when CFP was used as the matrix. Additionally, chemical image qualities closely followed the trend of S/N ratios. CFP proved to be an effective one‐step development and matrix application technique for MALDI‐MSI of latent fingerprints, when carbon cluster peaks are well separated by a HRMS.