Y-STRs in forensic medicine: DNA analysis in semen samples of azoospermic individuals

ABSTRACT: The incidence of rape has increased, especially in metropolitan areas, such as the city of São Paulo. In Brazil, studies about it have shown that the majority of this type of crime is committed by the relatives and persons close to the victim. This has made the crime more difficult to be denounced, as only 10% of the cases are reported to competent police authorities. Usually, cytological exams are carried out in sex crime investigations. The difficulty in showing the presence of spermatozoa is frequent, but it does not exclude the presence of male DNA. The absence of spermatozoa in material collected from rape victims can be due to several factors, including the fact that the agressor suffers from azoospermia. This condition can be the result of a successful vasectomy. As the majority of DNA in the ejaculation sample is from spermatozoa, there is much less DNA to be analyzed. This study presents the application of Y‐STRs (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, and DYS393) in DNA analysis of sperm samples from 105 vasectomized men. The study demonstrated a great variation in DNA concentration. DNA extraction and amplification was possible in all sperm samples even in the absence of spermatozoa. The same profile was observed, for each individual, from DNA extracted from blood, pre‐ and postvasectomy semen samples. The use of markers specific for Y chromosome in sex crime cases, especially in the absence of spermatozoa, is very important, mainly because in most situations there is a small quantity of the agressor's DNA in the medium and a large quantity of the victim's DNA.     

Virtual autopsy and forensic identification-practical application: a report of one case

The body of an unidentified elderly woman was found trapped in a floodgate. Prior to autopsy, full-body multislice computed tomography (MSCT) was performed for study of bone lesions and cause of death. Age was estimated by analysis of the sternal end of the fourth rib and of the pubic symphyseal medial articular surfaces. The results were then compared with the autopsy findings. MSCT was superior to autopsy in diagnosis of traumatic bone lesions and also revealed dental anomalies and signs of drowning. Age estimation gave a similar result for both methods. This case report illustrates the potential value of MSCT for medico-legal investigations of death: diagnosis of injuries, possibility of determining the cause of death, and anthropological study in order to estimate age or to visualize features likely to enable identification of a corpse.     

Violence,Guns, and Suicide in New Orleans: Results from a Qualitative Study of Recent Suicide Decedents

Many Americans own guns to protect themselves against other people, but there is evidence that both victimization and gun access increase suicide risk. We conducted qualitative interviews with informants of 17 suicide cases in New Orleans of the 60 who died between January 2015 and April 2016 to understand the relationship between past trauma, gun access and storage, and suicide. Nine cases had experienced a past trauma, including three who had recently had a family member killed by homicide. Eight died via firearm; of those, seven owned the guns they used to take their lives and stored them locked (but loaded) at home or in their cars. Preventing community violence and addressing its sequelae may be important for reducing suicides. A multi‐pronged strategy consisting of policies, education, and marketing will likely be needed to address the risk of suicide conferred by gun access.     

Parents’ Perspectives on Family‐centered Care in Juvenile Justice Institutions

Family‐centered care during adolescent detention aims to increase parental participation in an attempt to optimize treatment outcomes. However, little is known about parents’ needs in family‐centered care. To fill this gap, we interviewed 19 purposefully selected parents of detained adolescents using a semi‐structured topic list. Although needs differed between parents, they were generally interested in activities that included spending time with their child. It is important for parents to receive timely information about their child's condition and treatment, detention procedures, and activities in the facility. The outcomes demonstrated that parents expected a two‐way communication based on respect and reliability.     

X‐ray Diffraction and Rietveld Refinement in Deferrified Clays for Forensic Science

Soil vestiges might provide information about a crime scene. The Rietveld method with X‐ray diffraction data (RM‐XRD) is a nondestructive technique that makes it possible to characterize minerals present in the soils. Soil clays from the metropolitan region of Curitiba (Brazil) were submitted to DCB treatment and analyzed using XRD with CuK_α radiation in the step‐scan mode (0.02° 2θ/5 s). The GSAS+EXPGUI software was used for RM refinement. The RM‐XRD results, together with the principal component analysis (PCA) (52.6% total variance), showed the kaolinite predominance in most analyzed samples and the highest quartz contents in “site 1.” Higher anatase, and gibbsite and muscovite contents influenced discrimination, mainly in “site 3” and “site 1,” respectively. These results were enough to discriminate clays of four sites and two horizons using a reduced amount of sample showing that the technique can be applied to the investigation into soil vestiges.     

Generalizability of a two-factor measure of young children's suggestibility in Norway and the USA

Abstract

We discuss the development of the Book Suggestibility Scale for Children (BSSC), a two-factor suggestibility measure developed as an alternative to the Video Suggestibility Scale for Children (VSSC; Scullin & Ceci, Personality and Individual Differences, 30, 843–856, 2001). In study 1, both the BSSC and the VSSC were administered to 60 American preschool children. Children responded affirmatively to inaccurate leading questions (Yield) and changed their answers in response to negative feedback from interviewers (Shift) in a similar manner on both scales. In study 2 we found that the BSSC was useful for studies of suggestibility in contexts other than America because 62 Norwegian preschool children responded to questions in a similar manner to an age-range and vocabulary matched group of 31 American children. Results support a two-factor model of suggestibility, although Yield appears to measure suggestibility across scales and situations more consistently than does Shift. American age-matched samples had significantly higher Shift and Yield 2 scores than the Norwegian sample.     

Disturbed sleep as a risk factor for aggression in prisoners with a psychotic illness: a brief report

The objective of the present study is to examine whether a relationship exists between disturbed sleep and aggression in 19 male prisoners with a psychotic illness, incarcerated in the Penitentiary Psychiatric Centre in Amsterdam, the Netherlands. The sleep–wake rhythm was indirectly assessed by means of actigraphy. The results show that nocturnal restlessness was a significant predictor of aggression/agitation with a large effect size (R^2?=?.639, adjusted R²?=?.536). It was concluded that future research should focus on nocturnal restlessness as a possible cause of aggression in this population.     

Book reviews

Deane Curtin, Chinnagounders’ Challenge: The Question of Ecological Citizenship. Bloomington: Indiana University Press, 1999.

Jim Yong Kim, et ah, ed., Dying For Growth: Global Inequality and the Health of the Poor. Monroe, ME: Common Courage Press, 2000.

Lester R. Brown, Christopher Flavin, Hilary French, et al., eds., State of the World 1998. New York: W.W. Norton and Company, 1998.

Margit Mayer and John Ely, eds., The German Greens. Paradox Between Movement and Party. Translated by Michael Schatzschneider. Philadelphia: Temple University Press, 1998.