Sequencing of mitochondrial DNA and the problem of human specificity

When analysing trace materials and degraded DNA the issue of human specificity is highly important. Especially when it comes down to the analysis of mitochondrial DNA which is extremely susceptible to contamination authenticity is the main question. Therefore in the presented study mitochondrial primers were tested on their human specificity. In all cases it was possible to amplify DNA of animals with human mt-primers. These unintentional amplifications could only be decreased by choosing austere PCR parameters. The study implies the importance of comprehensive evaluation of primers, chemicals and PCR parameters.     

Thinking for Thousands: Emerson's Theory of Political Representation in the Public Sphere

This article develops Emerson's theory of representative democracy as it applies to a deliberative public sphere. By highlighting the democratic content of Emerson's thought, this article challenges tradition readings of Emerson that claim his thought to be elitist or antipolitical. According to Emerson, the public sphere is structured by representative individuals who are analogous to those representatives found in electoral institutions. These representatives make public the beliefs and values present in their "constituencies." They deliberate in the name of their constituencies, saying what their constituencies could and would say, were they to also directly engage in such deliberations. Representative individuals are tied to their constituencies through bonds of "sympathy and likeness." The moral consequences of a representative public sphere include the development of a sense of deliberative justice on the part of the citizenry and the reduction of the possibility of domination and oppression by ideologically oriented elites.