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What is the best sample for determining the early postmortem period by on-the-spot flow cytometry analysis? 总被引:8,自引:0,他引:8
Di Nunno N Costantinides F Cina SJ Rizzardi C Di Nunno C Melato M 《The American journal of forensic medicine and pathology》2002,23(2):173-180
The level of degradation of DNA as a means for determining the time of death has been proposed as a valid adjunct to the classic thanatochronologic methods. The twofold aim of this work was to determine which organ might reveal both a correlation between the percentage of degradation of the DNA and the time lapse since death, and would be easiest to sample and yield the most reproducible results even in technically unfavorable situations such as on-the-spot investigations at the scene of death. A comparison of the spleen, blood, and liver showed that hepatic tissue best meets these specific needs because it shows a virtually linear correlation between the time elapsed since death and the level of degradation of the DNA, and it can easily be sampled at the scene of death by use of a common biopsy needle. 相似文献
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Samyn N De Boeck G Wood M Lamers CT De Waard D Brookhuis KA Verstraete AG Riedel WJ 《Forensic science international》2002,128(1-2):90-97
In a double-blind placebo controlled study on psychomotor skills important for car driving (Study 1), a 75 mg dose of +/- 3,4-methylenedioxymethamphetamine (MDMA) was administered orally to 12 healthy volunteers who were known to be recreational MDMA-users. Toxicokinetic data were gathered by analysis of blood, urine, oral fluid and sweat wipes collected during the first 5h after administration. Resultant plasma concentrations varied from 21 to 295 ng/ml, with an average peak concentration of 178 ng/ml observed between 2 and 4h after administration. MDA concentrations never exceeded 20 ng/ml. Corresponding MDMA concentrations in oral fluid, as measured with a specific LC-MS/MS method (which required only 50 microl of oral fluid), generally exceeded those in plasma and peaked at an average concentration of 1215 ng/ml. A substantial intra- and inter-subject variability was observed with this matrix, and values ranged from 50 to 6982 ng/ml MDMA. Somewhat surprisingly, even 4-5h after ingestion, the MDMA levels in sweat only averaged 25 ng/wipe. In addition to this controlled study, data were collected from 19 MDMA-users who participated in a driving simulator study (Study 2), comparing sober non-drug conditions with MDMA-only and multiple drug use conditions. In this particular study, urine samples were used for general drug screening and oral fluid was collected as an alternative to blood sampling. Analysis of oral fluid samples by LC-MS/MS revealed an average MDMA/MDEA concentration of 1121 ng/ml in the MDMA-only condition, with large inter-subject variability. This was also the case in the multiple drug condition, where generally, significantly higher concentrations of MDMA, MDEA and/or amphetamine were detected in the oral fluid samples. Urine screening revealed the presence of combinations such as MDMA, MDEA, amph, cannabis, cocaine, LSD and psilocine in the multiple-drug condition. 相似文献
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本文评述了美国与东亚大国———中国、日本、俄罗斯、印度及东盟的关系,以及这些关系对东亚的影响,并着重分析了中国与东亚大国的关系。作者认为,中美关系肯定是未来东亚地区最重要的战略关系。中国的崛起促使创立东亚地区机制成为必要。多边合作是解决和克服双边低效的最直接的战略,因此,创立东亚共同体的想法具有很重要的意义。 相似文献
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应暨南大学的邀请,2006年5月27-30日,印尼前国家领导人———印尼人民协商会议(MPR)主席、印尼国民使命党前任总主席、穆罕默迪亚前任总主席、现印尼国民使命党荣誉主席、印尼知名学者阿敏.赖斯教授(Prof.Dr.HM Amien Rais)对暨南大学进行了为期3天的学术访问,并被聘请为暨南大学客座教授。期间,阿敏.赖斯教授做了两场学术报告,分别就印尼民主化进程、中国印尼关系、国际形势等诸多问题发表了看法。参加学术报告会的有暨南大学东南亚研究所暨国际关系学系师生、印尼留学生、广东外语外贸大学印尼语专业师生和广州市、中山市、珠海市的印尼归侨代表等130人,东南亚研究所所长曹云华教授主持了学术报告会。现对其主要观点和看法进行整理,以飨广大 相似文献