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31.
This study presents a reliable method that uses high-fidelity long-range PCR and optimized primers to assess polymorphism and to genotype human mitochondrial DNA (mtDNA). This method was used to analyze polymorphic sites in the human mtDNA control region, including hypervariable regions I, II, and III (HVI, HVII, and HVIII), from 124 unrelated Japanese individuals. In HVI, HVII, and HVIII, 80, 37, and 14 polymorphic sites were identified, respectively, excluding those in the homopolymeric cytosine stretch (C-stretch) regions. The region between HVI and HVII also contained 15 polymorphic sites. On the other hand, C-stretch length heteroplasmy in HVI or HVII was observed in 66 of 124 Japanese individuals (53%), which is much higher than in Caucasian populations. The variants in the C-stretch regions were characterized by counting the number of heteroplasmic peaks split from the single peak in homoplasmic sequences (i.e., 16244G and 16255G in HVI and 285G in HVII). Including the C-stretch length heteroplasmy, the 124 Japanese mtDNA samples were classified into 116 distinct haplotypes. The random match probability and the genetic diversity were estimated to be 0.95% and 0.998581, respectively, indicating that the method presented here has higher discrimination than the conventional method for mtDNA typing using HVI and HVII. [Correction added after publication 30 January 2007: in the preceding sentence random match probability and genetic diversity estimates were corrected from 0.95 and 0.998581%, respectively, to 0.95% and 0.998581, respectively.] The haplogroups and their frequencies observed in this study (i.e., D4; 13.7%, M7a1; 11.3%, D4a; 9.7% and M7b2; 8.9%) were similar to those observed in other studies of Japanese mtDNA polymorphism. The method described here is suitable for forensic applications, as shown by successful analysis of tissues from highly putrefied remains of an infant, which allowed maternal relationship to be determined via mtDNA haplotyping. 相似文献
32.
从1991年教育法规部发动两次改革以来,日本的高校学术评价体系发生了巨大的变化。其中,最显著的变化是其评价体系从自我评价向第三方评价转换。在这一过程中,全国学位与大学评价协会发挥了重要的作用。 相似文献
33.
Akira Inoue 《Law and Philosophy》2007,26(6):575-587
According to a renowned left-libertarian, Michael Otsuka, a libertarian right of self-ownership can be so robust that one
need not sacrifice the use of one's mind and body to help others. In this article, I demonstrate that Otsuka's way of reconciling
this robust conception of self-ownership with equality is not appealing and, at best, would provide limited guidance in the
face of real-life uncertainty. 相似文献
34.
Quantitative analysis of tropane alkaloids in biological materials by gas chromatography-mass spectrometry 总被引:2,自引:0,他引:2
Namera A Yashiki M Hirose Y Yamaji S Tani T Kojima T 《Forensic science international》2002,130(1):34-43
A simple and rapid method for quantitation of tropane alkaloids in biological materials has been developed using an Extrelut column with gas chromatography-mass spectrometry (GC-MS). Biological materials (serum and urine) were mixed with a borate buffer and then applied to an Extrelut column. The adsorbed tropane alkaloids were eluted with dichloromethane before a GC-MS analysis. Atropine-d(3) was used as an internal standard. The extracted tropane alkaloids were converted to trimethylsilyl derivatives prior to GC analysis, to improve the instability of tropane alkaloids from heating and the property of them for a GC column. The recoveries of the compounds, which had been spiked to biological materials, were more than 80%. The GC separation of the derivatives from endogenous impurities was generally satisfactory with the use of a semi-polar capillary column. Tropane alkaloids showed excellent linearity in the range of 10-5000 ng/ml and the limit of detection was 5.0 ng/ml for biological materials. The present method is simple and more rapid than those previously reported, and was applied to a poisoning case. It is useful for the routine analysis of tropane alkaloids in cases of suspected tropane alkaloids poisoning. 相似文献
35.
Kinoshita H Taniguchi T Nishiguchi M Ouchi H Minami T Utsumi T Motomura H Tsuda T Ohta T Aoki S Komeda M Kamamoto T Kubota A Fuke C Arao T Miyazaki T Hishida S 《Forensic science international》2003,133(1-2):107-112
We present here a fatal poisoning case involving verapamil, metoprolol and digoxin. A 39-year-old male was found dead in his room, and a lot of empty packets of prescribed drugs were found near the corpse. The blood concentrations of verapamil, metoprolol and digoxin were 9.2 microg/ml, 3.6 microg/ml and 3.2 ng/ml, respectively. The cause of death was given as cardiac failure, hypotension and bradycardia due to a mixed drug overdose of verapamil, metoprolol and digoxin, based on the results of the autopsy and toxicological examination. We speculate that the toxicity of verapamil is potentiated by drug interaction with metoprolol and digoxin. 相似文献
36.
Akira Kawamoto 《Asia-Pacific Review》1998,5(1):83-95
Economic regulation that aims to restrict competition should in principle be eliminated, says Akira Kawamoto, principal administrator in charge of the Regulatory Reform Project in the Trade Directorate of the OECD. Competition is the key to reform: there is increasing evidence that regulatory reform that opens domestic markets to vigorous international competition results in improved economic efficiency and greater productivity. However, implementing reform is not easy, and lasting political will, skillful management, and precise technical knowledge are required if the difficulties are to be overcome, Kawamoto says. This paper was given at the IIPS conference on “Challenges of globalization” that was held in December 1997. 相似文献
37.
Heino F. L. Meyer-Bahlburg Anke A. Ehrhardt Jennifer J. Bell Susan F. Cohen Jane M. Healey Judith F. Feldman Akira Morishima Susan W. Baker Maria I. New 《Journal of youth and adolescence》1985,14(4):339-353
A promising model syndrome for the examination of the role of physical maturation in the development of female sexuality is idiopathic precocious puberty (IPP). In this first controlled study of psychosexual development in IPP females, 16 females between 13 and 20 years of age with a history of IPP were compared to 16 control subjects with a history of normal puberty pair-matched to the index subjects on the basis of sex, race, age, socioeconomic level, and menarcheal status. The psychosexual history and the current psychosexual status were assessed by a systematic half-structured interview. The IPP females on average passed the psychosexual milestones at an earlier age than their normal maturing peers, with a particularly early onset of masturbation. Those who were sociosexually active tended to report a higher total orgasmic outlet and a higher sex drive. There was no increase in homosexuality among IPP girls. The timing of puberty has a (modest) influence on psychosexual development in females.This work was supported by NIMH Center Grants MH 30906-01A and MH 30906-03 and by a grant from the Spencer Foundation. 相似文献
38.
Akira Kurosu M.D. Ph.D. Kazumi Kuroyanagi M.D. Shinobu Yamauchi Ph.D. Kazunobu Omura Ph.D. Kazuyuki Saito M.D. Ph.D. 《Journal of forensic sciences》2016,61(2):548-550
Anomalous origin of the coronary artery (AOCA) is a rare, but important cause of sudden cardiac death among young athletes. Nine autopsy cases (8 male, 1 female; mean age, 17.9 years; age range, 11–31 years) of sudden death during or just after exercise caused by AOCA were reviewed. The exercises performed at the time of death were running (4 cases), soccer (2 cases), and baseball, swimming and kendo (Japanese swordsmanship) (1 case each). In 6 cases, the left coronary artery arose from the right sinus of Valsalva, and in 3, the right coronary artery from the left sinus. The coronary arteries passed between the pulmonary artery and the aorta with an acute angle takeoff from the orifice. Three cases had cardiovascular manifestations prior to death. In cases with cardiovascular manifestations, novel imaging methods should be considered to prevent sudden death. 相似文献
39.
Akira Suzuki 《当代亚洲杂志》2016,46(4):591-613
This article analyses how diverse stances toward nuclear energy were produced and reproduced among Japanese labour unions from the mid-1960s to the mid-1980s, and how these stances of the formative period influenced the subsequent development of inter-union politics before and after the Fukushima nuclear plant disaster in 2011, based on content analysis of union bulletins and other materials of pro- and anti-nuclear energy unions. This article asks three research questions: (i) what factors contributed to pro- or anti-nuclear energy stances of labour unions?; (ii) what elements constituted or framed unions’ arguments on the issue of nuclear energy?; and (iii) what impact did these stances and arguments formed in the early period have on the subsequent development of inter-union politics over nuclear energy, especially in the aftermath of the Fukushima nuclear disaster? The paper shows union ideologies and institutional integration were the factors influencing unions’ stances on nuclear energy. It also analyses the worldviews of pro- and anti-nuclear energy unions based on the three policy styles of technological enthusiasts, moralists and cost-benefiters. The Fukushima disaster reinforced the previously formed division of union ideologies, but considerably weakened the discourse of technological enthusiasm stressed by pro-nuclear energy unions. 相似文献
40.