Analysis of sensitivity and specificity: precise recognition of neutrophils during regeneration of contused skeletal muscle in rats

In this report, we applied the TissueFAXS 200 digital pathological analysis system to rapidly and accurately identify neutrophils during regeneration of contused skeletal muscle, and to provide information for follow-up studies on neutrophils to estimate wound age. Rat injury model was established, and skeletal muscle samples were obtained from the control group and contusion groups at 1, 1.5, 2, 3, 4, and 6 h, as well as at 1, 3, 5, and 15 d post-injury (n = 5 per group). The expression of nuclei and neutrophils was detected by hematoxylin and eosin (HE) staining and immunohistochemical (IHC) staining. A total of 20 injury site areas of 0.25 mm² (0.5 mm × 0.5 mm) were then randomly selected at all time points. A TissueFAXS 200 digital pathological analysis system was used to identify the positive and negative numbers. Knowledge of five professional medical workers were considered the gold standard to measure the false positive rate (FPR), false negative rate (FNR), sensitivity, specificity, and area under the curve (AUC) of receiver operating characteristic (ROC) curves. As a result, with a staining area of neutrophils from 8 µm² to 15 µm², the FPR was 4.28%–12.14%, the FNR was 12.42%–64.08%, the sensitivity was 35.92%–87.58%, the specificity was 87.86%–95.72%, the Youden index was 0.316–0.754, the accuracy was 82.80%–88.30%, and the AUC was 0.771–0.826. The AUC was largest when the cut-off value of the staining area was 12 µm². Our results show that this software-based method is more accurate than the human eye in evaluating neutrophil infiltration. Based on the sensitivity and specificity, neutrophils can be accurately identified during regeneration of contused skeletal muscle. The TissueFAXS 200 digital pathological analysis system can also be used to optimize conditions for different cell types under various injury conditions to determine the optimal cut-off value of the staining area and provide optimal conditions for further study. Furthermore, it will provide evidence for forensic pathology cases.     

哈马斯现象的宗教-政治解读

以伊斯兰主义相号召,在广大信众中深深扎下了根是哈马斯能够在以色列武力镇压中立于不败之地的重要原因。哈马斯不仅仅是一个政治组织和武装组织,更不仅仅是少数持较为极端立场的人所组成的集团,而是成千上万由伊斯兰主义武装起来的信众的代表,体现已经联合起来、且已形成强大声势的抵抗运动。在立法委员会选举前哈马斯便已取代法塔赫,成为最受巴勒斯坦人欢迎的巴政治派别。它在立法委员会选举中获胜反映了选民对以色列的强烈不满与对巴解组织的失望。     

A Candid Conversation

Messages from the China-U.S.engagement in Anchorage While the first China-U.S.senior officials'meeting in Alaskan city Anchorage since U.S.President Joe Biden took office has been described as chilly by the Western media,to look at it objectively,the March 18-19 strategic dialogue has clarified the red lines on both sides and broken the ice for further communication.     

A Conflict of No Good

<正>The India-provoked border incident benefits no one Sino-Indian relations are e xperiencing their most severe standoff since the two nations’border war 55 years ago.In late June,Indian troops trespassed into China in the Donglang area at the Sikkim section of the China-India boundary.According to historical documents of the two countries,the     

优化空间结构 助推黔中城市群发展

文章利用2000-2009年统计数据对黔中城市群经济发展水平进行分析,采用首位度等指标对黔中城市群等级规模结构进行探讨。研究发现,黔中经济区初步具备城市群发育的基础。但是,黔中城市群存在总体的城市数量偏少、大城市缺位、中小城市数量不足、黔中城市群的城市规模等级结构不合理等问题。对于大城市不足的黔中地区而言,比较现实的城镇化发展路径,可能是一方面增强特大城市的辐射力;另一方面重点改善城市群内中小城市的基础设施条件和投资环境,改善城市间交通运输条件,帮助它们发展成为较大城市;从而逐步形成一个良性的城市群结构。     

A Two‐Level Theory of Presidential Instability

This article analyzes the conditions that facilitate the ousting of Latin American presidents and the mechanisms that prevent their downfall. Drawing lessons from the impeachment of Paraguayan president Fernando Lugo, it extends previous arguments about the “legislative shield” to show that the same forces that sometimes conspire to terminate an administration at other times work to resist its demise. The argument underscores the interaction between legislators and social movements, two prominent actors in the literature on presidential instability. The article presents a two‐level theory to identify possible configurations of mass and legislative alignments, and tests some implications of the theory with data for 116 Latin American presidents over 28 years. Multiple comparison tests based on random effects logistic models show that popular protests can be neutralized by strong support in Congress, and hint at the possibility that legislative threats can be neutralized by loyal demonstrators in the streets.     

协商共治:建构农村基层治理的制度性合作关系

村民自治在我国的出现,是农村经济体制改革后国家与社会权力关系调整的制度结果.但村民自治在实施过程中体现出了一定的制度限度与困境,其背后所反映的是当前我国农村基层治理权力格局中多元主体间的结构性张力.在此背景下,建构多元治理主体的制度性合作关系,实现其合作共治是农村基层善治的重要途径.在我国传统协商治理以及西方协商民主理念下建立起协商参与机制,则是实现这一制度性合作关系的现实选择.     

略论语境对英语词义理解的作用

习得词汇的方法很多，其中借助英语语言性语境和非语言性语境理解并把握词义，不失为一种行之有效的学习方法。     

高效液相色谱法测定三七及三七片中人参皂苷Rg1的含量

目的:建立三七及其制剂三七片中人参皂苷Rg1的含量测定方法。方法:采用反相高效液相色谱法,色谱柱为Teknokroma Kromasil C18(5μm,2.1 cm×15 cm),以乙腈∶0.5 g/L磷酸水溶液(21∶79)为流动相,流速为0.3 ml/min,检测波长为203 nm,柱温为30℃。结果:人参皂苷Rg1浓度为21.4~534μg/ml时,线性关系良好(r=0.9991);平均回收率为99.3%,RSD=1.47%(n=6)。结论:本方法准确、快速、稳定、重现性好,可用于三七药材及其制剂的质量控制。     

中国教育学的当下使命

中国教育学当前主要存在"西方化"、"唯科学化"和漠视教育实践等三方面的问题.当下中国教育学的使命:在研究思路上,将国际理论视野和中国问题意识进行创造性的整合;在价值导向上,从"知识世界"回归"人的世界";在实践追求上,以解决教育问题,影响教育决策为旨趣.只有这样,中国的教育学才能为世界学术做出自己独特的贡献.