工程建设领域犯罪行为及刑事责任类型化研究

随着我国工程建设范围的不断扩大,各类犯罪行为也时有发生。通过对1998年至2019年期间所涉的各类犯罪行为进行实证分析,并对工程建设领犯罪类型化的必要性和可行性进行研究,发现我国工程建设领域中主要存在工程事故类犯罪、工程贪贿类犯罪、工程诈骗类犯罪、工程生态类犯罪、工程侵权类犯罪、工程渎职类犯罪六类犯罪。应针对此六类犯罪开展类型化研究,以期达到有效缓解工程建设领域犯罪的目的。     

公务员精神与公务员制度

公务员精神来源于公务员制度的本质规定。具有中国特色的社会主义公务员制度决定了中国公务员精神与西方公务员精神既有相同之处,又在“政治中立”这一点上有着根本的区别。随着中国特色社会主义公务员制度的不断完善,公务员精神的内涵也应当不断丰富和发展。公务员精神体现了公务员制度中的爱国主义、集体主义、职业精神和时代精神。当前最为迫切的就是以个人自律、组织教育、制度控制、环境制约等为主要途径,从精神、制度与方法的有机结合上促使每个公务员真正成为“公务员精神”的实践者和体现者。     

省级政府大数据发展应用政策的规制导向与执行优化研究——基于政策文本的分析

省级政府大数据发展应用政策是大数据政策体系中极具实践指向和促进功能的专项政策,发挥着"上承中央-下启基层"的衔接互动作用,对其进行规制导向分析可充分呈现其内部执行要素的分布情况,亦可为其实施评估及纠偏矫正提供更加细致可靠的理论依据.通过构建政策执行综合模型,运用政策文本分析方法,本文从"政策问题的可处理性""政策协调能...     

筹建亚洲基础设施投资银行的基本思路及对策建议

为促进亚洲地区互联互通建设和经济一体化进程,中国政府倡议筹建亚洲基础设施投资银行,得到东盟的赞赏和支持。筹建亚洲基础设施投资银行已经提上重要议事日程。本文拟阐述筹建亚洲基础设施投资银行的重大意义,分析筹建亚洲基础设施投资银行面临的困难和挑战,研究提出筹建亚洲基础设施投资银行的基本思路,建议筹建亚洲基础设施投资银行率先在中国—东盟区域实现突破,取得好的成效、积累一定经验后再逐步扩大到整个亚洲地区。本文设计了亚洲基础设施投资银行的性质宗旨与职能定位、机构设置与总部选址、资金来源与合作方式等,尤其对在中国—东盟区域起步的亚洲基础设施投资银行的合作方式、期初的股本资金、各国出资金额及所占比重进行较为深入的研究,提出可行性初步操作方案,抛砖以引玉,供决策参考。需要说明的是,本文提出的观点仅是我们个人学术观点,不代表任何组织和机构。     

From national identity to national security: China's changing responses toward India in 1962 and 1998

This article examines Russian Government policy towards Northeast Asia as an interplay between aspirations, which have been expressed by government leaders, parliamentary figures and prominent academics and journalists and actual results. The article uses three levels, global, regional and bilateral, as a basis for the analysis. In terms of global interests the Russian leadership has sought diplomatic balance against the US, in terms of regional interests the leadership has pursued the aim of economic and security integration while at the bilateral level Russian leadership has sought specific benefits from particular relations with China, Japan and South Korea. The article notes that Russia has obtained certain diplomatic benefits from the relationship with China which serve its interests at the global level. At the regional level policy aspirations have been frustrated by Russia's dire economic condition and the tendency to be treated as an ‘outsider’. Russia has benefited from an improvement of bilateral relations with China, Japan and South Korea but these gains do not translate into an improved position at the regional level yet. while the Russian leadership has been nurturing the development of bilateral relationships problems particular to those relationships hinder its wider regional acceptance.     

古琴与中国传统礼乐文化

我国传统文化是以"和"为特色的礼乐文化,大致可分为"和天地"、"和鬼神"、"和家邦"、"和身心"几个阶段。古琴在传统礼乐文化的嬗变过程中充当着不可或缺的角色,从一定意义上甚至可以说,古琴浓缩了礼乐文化的整体特征,是我国传统文化的载体。     

Preventive servicing is good for business and affordable homeownership policy

Abstract

This article documents the growing importance of preventive servicing—business practices that emphasize early intervention in delinquency and default management practices that also help financially troubled borrowers avoid foreclosure. We suggest that the loan servicing side of the affordable housing delivery system may be underappreciated and undercapitalized.

We use a database of more than 28,000 affordable housing loans to test several preventive servicing‐related propositions and find that after we control for loan and borrower characteristics, the likelihood that a delinquent mortgagor within this universe will ultimately default varies significantly across servicers. This suggests that loan servicing is an important factor in determining whether low‐ and moderate‐income borrowers who fall behind in their mortgage payments will end up losing their homes through foreclosure. It also suggests a need for policy makers to incorporate preventive servicing into affordable homeownership programs.     

鸡毒支原体醛缩酶的原核表达及膜定位鉴定

为了探明醛缩酶在支原体中的定位,根据已发表的鸡毒支原体醛缩酶(fba)基因序列设计特异性的引物,以鸡毒支原体Rlow株基因组为模板,通过Overlap PCR点突变扩增鸡毒支原体醛缩酶fba基因,将fba克隆至pET-28a(+)载体后进行序列测定和分析。结果表明,fba基因全长873bp,编码290个氨基酸。将构建的重组表达质粒pET28a-fba转化至大肠杆菌BL21(DE3),在IPTG诱导下成功获得表达融合蛋白rMGfba,大小约为33ku。纯化蛋白并对蛋白进行酶活性检测,结果显示该酶在体外具有与阳性对照醛缩酶相同的催化功能。用该蛋白免疫小鼠制备多克隆抗体,提取鸡毒支原体膜蛋白,Western-blot分析结果显示FBA多抗可与疏水性膜蛋白特异性结合,说明FBA位于鸡毒支原体膜表面。     

猪传染性胃肠炎病毒S蛋白D位点单克隆抗体的制备和鉴定

通过大肠杆菌表达了猪传染性胃肠炎病毒(TGEV)S蛋白的D位点,并以D位点的六聚体作为抗原,免疫BALB/c小鼠,取免疫后小鼠脾细胞和骨髓瘤细胞SP2/0进行融合,经间接ELISA筛选和3次有限稀释法进行克隆。结果显示,得到3株能稳定分泌抗TGEV S蛋白D位点单克隆抗体的杂交瘤细胞株,分别命名为2C104、5E1012和4B101,其中2C104和5E1012分泌抗体类型是IgM亚类,4B101为IgG1亚类。2C104、5E1012、4B101三株杂交瘤细胞培养上清液的间接ELISA抗体效价分别为1∶128、1∶128、1∶64,腹水效价分别为1∶103、1∶104、1∶4×103。间接免疫荧光鉴定结果表明,3株细胞均能和TGEV发生特异性反应,且在连续传代和冻存后仍能稳定分泌抗体。本研究针对S蛋白D位点制备了单克隆抗体,为传染性胃肠炎的诊断和TGEV与宿主互作机制的研究奠定了基础。     

05式警用转轮手枪弹壳接触DNA检验方法的比较

目的比较05式警用转轮手枪弹壳表面接触性DNA检验方法,为实际检验提供参考和借鉴。方法制备40例击发后手枪弹壳的模拟样本,分别用两步转移法提取弹壳表面不同部位检材,采用两种DNA提取法和两种扩增试剂盒对样本进行STR分型检验,比较评价检验结果。结果避开发射药残留区域采用两步转移法提取样本,有助于提高检出率;Chelex-100联合Microcon-100法提取模板DNA的产量最高可达1.18ng,高于Mini M48试剂盒法(0.91ng);MiniFilerTM试剂盒的等位基因检出率(23.61%)高于IdentifilerTM试剂盒(6.41%)。结论采用选择适当区域提取检材,采用Chelex-100联合Microcon-100法提取DNA,经MiniFilerTM试剂盒扩增,进行弹壳接触DNA分型的效果较好。