Characterization of 26 miniSTR loci for improved analysis of degraded DNA samples

An additional 20 novel mini-short tandem repeat (miniSTR) loci have been developed and characterized beyond the six previously developed by our laboratory for a total of 26 non-CODIS miniSTR markers. These new markers produce short PCR products in the target range of 50-150 base pairs (bp) by moving the primer sequences as close as possible-often directly next to the identified repeat region. These candidate loci were initially screened based on their small amplicon sizes and locations on chromosomes currently unoccupied by the 13 CODIS STR loci or at least 50 Mb away from them on the same chromosome. They were sequenced and evaluated across more than 600 samples, and their population statistics were determined. The heterozygosities of the new loci were compared with those of the 13 CODIS loci and all were found to be comparable. Only five of the new loci had lower values than the CODIS loci; however, all of these were much smaller in size. This data suggests that these 26 miniSTR loci will serve as useful complements to the CODIS loci to aid in the forensic analysis of degraded DNA, as well as missing persons work and parentage testing with limited next-of-kin reference samples.     

At-Large Elections and Minority Representation in Local Government

Despite a long history of legal challenges alleging that elections conducted at-large suppress minority representation, this remains the dominant electoral system in local governments throughout the United States. Moreover, a large empirical literature remains divided over the present-day impact of at-large elections on the political success of underrepresented groups. We reconcile the competing findings in this literature by providing contingent, causal estimates of the effect of conversion from at-large to ward elections on minority officeholding, using a novel identification strategy afforded by the California Voting Rights Act of 2001. We find a dramatic positive effect of conversion in districts where Latinos constitute a sufficiently large share of the voting population, and in large and residentially segregated districts. When these conditions are not satisfied, we consistently see null estimated effects.     

Federation and Australian Nationalism: Early Commemoration of the Commonwealth

Much has been written about the nature of Australian nationalism during the late nineteenth and early twentieth centuries. While post-Second World War historiography analysed the role of class in nationalist sentiment, more recent work has examined the racialised and martial aspects of Australian nationalism and imperialism. There has been less consideration of how the nature of turn-of-the-century Australian nationalism affected the Federation that was established on 1 January 1901. This article examines early debates about commemoration of the anniversary of Federation, revealing an indifference to the occasion that was common to the public and most political and civic leaders, including Prime Minister Edmund Barton. It finds that popular enthusiasm at the inauguration of the Commonwealth in January 1901 and the opening of the first parliament in May was a response to imperial pageantry and celebrity, rather than the creation of the Australian federation. The article suggests that Australians’ longstanding resistance to reform of the Federation is a legacy of their historic failure to attach to it.     

Older parents' expectations for filial support

A measure of general filial obligation expectations is presented. With data from a representative sample aged 70 and older, we show that generalized expectations for support from adult children are not patterned systematically by sociodemographic factors or by health needs of the older respondents. A typology is presented which relates expectations for help to judgments of the level received. This typology is meaningfully related to social indicators presumably related to need such as marital status and socioeconomic status. Whether one is dissatisfied, satisfied, or pleasantly surprised by the assistance one receives is associated with evaluative judgments of children. The parent-child bond is affected not only by exchange patterns but by the fit between expectations and exchange patterns.     

microRNA Detection in Blood,Urine, Semen,and Saliva Stains After Compromising Treatments

Evaluation of microRNA (miRNA) expression as a potential method for forensic body fluid identification has been the subject of investigation over the past several years. Because of their size and encapsulation within proteins and lipids, miRNAs are inherently less susceptible to degradation than other RNAs. In this work, blood, urine, semen, and saliva were exposed to environmental and chemical conditions mimicking sample compromise at the crime scene. For many treated samples, including 100% of blood samples, miRNAs remained detectable, comparable to the untreated control. Sample degradation varied by body fluid and treatment, with blood remarkably resistant, while semen and saliva are more susceptible to environmental insult. Body fluid identification using relative miRNA expression of blood and semen of the exposed samples was 100% and 94%, respectively. Given the overall robust results herein, the case is strengthened for the use of miRNAs as a molecular method for body fluid identification.     

Population size or population density? An empirical examination of scale economies in South Australian local government, 2015/16

ABSTRACT

Advocates of amalgamation typically claim that substantial scale economies flow from municipal mergers, which generate larger local government entities by population size. We examined whether economies of scale exist in council outlays by analysing the expenditure of 68 South Australian local government areas using data from the 2015/16 financial year. However, given the correlation between population size and population density it is important to determine whether the influence of population size on expenditure is due to variations in population density. We find that when local government areas are stratified into subgroups on the basis of population density, the evidence of economies of scale largely evaporates. From a policy perspective, this suggests that in place of municipal mergers, policymakers should instead explore avenues for shared service arrangements in those functions which exhibit scale economies.