鼠抗人转铁蛋白抗体轻链与重链可变区基因库的制备

以纯化的人转铁蛋白免疫小鼠,测其血清效价,合乎要求后杀鼠取脾脏并以异硫氰酸胍和酚的混合溶液将其匀浆,再以氯仿抽提、异丙醇沉淀和乙醇洗涤,所得总RNA以oligo(dT)纤维素离心柱分离纯化制备mRNA,使mRNA达到eDNA合成所需的浓度后,反转录制备出eDNA,在合成的eDNA中加入轻、重链引物,扩增制备出小鼠抗人转铁蛋白抗体的轻、重链可变区基因库。     

医疗纠纷法律适用若干问题探讨

处理医疗纠纷案件如何适用法律是司法机关深感困惑的一个问题。医疗纠纷的处理范围是在诊疗护理工作中,由于医务人员的诊疗护理过失,直接给患者造成不良后果所引起的争端;尽管医疗机构是提供医疗服务的单位,但患者不是消费者,医患纠纷不适用消费者权益保护法;医疗事故赔偿责任的法律适用应公正公平保护医患双方的合法权益。     

试论“教育体制决定教育”的局限性

单纯的教育体制改革活动的推进,将可能导致教育体制改革与教育观念、教育指导思想、社会化环境产生矛盾,而且从理论上难以解释一般教育现象、教育波动现象、教育失误现象等形成的原因。克服“教育体制完全决定教育”理论误区的国好思路为：建立教育机制理论。教育机制作为能够完全决定教育活动的组织和控制系统,包含了教育体制教育思想,教育化,社会化等一切决定和影响教育的要素,其理论不仅能清晰地说明教育活动的原因和     

鼠抗人转铁蛋白单链抗体基因序列测定及分析

从携带人转铁蛋白单链抗体基因载体转化的大肠杆菌菌株中提取质粒,经电泳分析及筛选、纯化出线状质粒DNA,定量后以载体上人转铁蛋白单链抗体基因插入子两边的已知DNA序列经合成后作为引物进行测序扩增凝胶电泳,将得到的人转铁蛋白单链杭体基因序列重轻链可变区部分同基因库中鼠抗体重轻链进行同源比对,证明人转铁蛋白单链抗体基因确由鼠抗体重轻链可变区基因构成,在此基础上,进一步对基因核苷酸序列的可变区结构特征作推断分析,并推导出其对应的氨基酸序列.     

论软件最终用户侵犯著作权之刑事责任

近年来,计算机软件“最终用户”的法律责任问题成了知识产权理论界关注的热点,“软件最终用户”“商业使用”侵权复制品的行为属于“复制或者部分复制著作权人的软件的侵权行为”已经成为理论界和实务界的共识。“最终用户”因此承担民事责任乃至受到著作权行政管理机关行政处罚的案例已经屡见不鲜。“最终用户”从最初的不承担侵权责任,到承担民事责任、行政责任,有没有可能承担刑事责任?《刑法》关于侵犯著作权犯罪的规定究竞能否适用于“最终用户”?这些问题至今没有引起理论界的重视,以至在执法、司法实践当中存在不少认识上的误区,行政执法机关遇到此类案件在是否向司法机关移交的问题上也是莫衷一是,左右两难。笔者根据工作中的体会,对这一问题进行初步探讨,以期引起学界关注,起到抛砖引玉的效果。     

Interpersonal and Personal Antecedents and Consequences of Peer Victimization Across Middle Childhood in Hong Kong

Although much is known about peer victimization, the majority of the longitudinal research in this area has been restricted to Western settings. The main objective of this study was to examine the interpersonal (rejection) and personal (withdrawal, aggression) antecedents and consequences of victimization for Chinese children living in Hong Kong. A sample of 1,058 children (501 boys; M age = 9.5 years) in Hong Kong was followed longitudinally from the 3rd and 4th grades to the 7th and 8th grades. Consistent with a transactional framework, rejection and withdrawal contributed to, as well as resulted from, victimization. Although victimization predicted later aggression, aggression was unrelated to later victimization. These findings closely replicate past research conducted in North America and European settings, and suggest considerable correspondence in the links between maladaptive child characteristics and victimization across Western and Hong Kong schools.     

The daiku phenomenon: social and cultural influences of Beethoven’s Ninth Symphony in Japan

In the rude awakening of western colonisations in Asia during the nineteenth century, Japan drastically embraced the ‘better and more modern’ western values towards the end of that century—which began with the Meiji Restoration (Meiji Ishin [ ]). Since the nation began frantically learning everything Western—while keeping its traditional values at heart, Beethoven’s Ninth Symphony ‘Choral,’ or daiku as it is known in Japan, has been present throughout the turbulent twentieth century. The social and cultural phenomenon known as nenmatsu-no daiku ([ ] A countless number of annual year-end Beethoven’s Ninth Symphony concerts held throughout Japan each year.) is the subject under scrutiny in this paper. Japan’s almost desperate pursue toward westernization and the much-debated Japanese cultural trait, the ‘groupism’ mentality would make the existence of the daiku phenomenon appear to the bewildered outsider as a horrendous mix of the two elements, but is that simply so? Evidently, the sudden influx of western cultures and ideologies from Europe and the U.S.A. is in great part accountable for the continual and prevalent existence of the daiku in modern Japan. However, we must not overlook the underlying home-grown factors, which have kept the phenomenon well and alive even today. This paper intends to discuss the historical background leading to this phenomenon and present a summary of what daiku is, giving examples of different types of performances as a result of the phenomenon. An attempt will also be given to examine the significance Beethoven’s Ninth has for the Japanese in both social and cultural contexts.     

任中共抚顺特支书记时的杨靖宇

2009年8月,是杨靖宇将军任中共抚顺特支书记80周年纪念,他是优秀的共产主义战士、人民军队的杰出将领、伟大的抗日民族英雄、东北抗日联军的创建人和领导人、建国后中共中央为个人举行公祭仪式第一人。谨以此文以示怀念。     

从可持续发展的角度看稳定中国的人口规模

当前中国实行的计划生育政策其实是一种压缩型人口政策,随着社会、经济的发展,以及计划生育本身作用的发挥,人口增长面临严重下滑趋势,不利于中国可持续发展,亟需引起重视。