钝性暴力致脾破裂的发生机制研究

脾破裂与外伤之间因果关系的判断是法医学鉴定中一个十分常见但又非常棘手的问题。探究脾破裂的机制是解决上述问题的必要的理论基础。本文收集了２５例钝性暴力致脾破裂的案例，对脾破裂的部位与暴力打击部位及方向的关系进行了分析、整理，探讨了人体各部位遭受暴力作用后脾破裂的特点，及钝性暴力导致脾破裂的机制，发现脾破裂的机制主要包括直接暴力冲击以及脾脏在受外力作用后撞击胃、隔肌、脊柱、肋骨和胸后壁等两类，并指出对脾破裂机制的进一步研究将成为法医学鉴定研究工作中的一项重要课题。     

冷战后欧盟对伊朗的政策

欧盟与伊朗关系是后冷战时代中东地区一对重要的双边关系。为了维护该地区的相对稳定和扩展自身的利益及影响力,欧盟对伊朗采取了不同于冷战后期(1979~1990年)的政策,逐步实施“以接触求转变”的政策。随着伊朗内政、外交的调整和中东地区形势的变动,该项政策在内容上经历了从“批评性对话”到“建设性对话”的转变,在解决伊朗核问题的方式上凸显与美国不同的外交取向。     

舆论监督司法的有效模式

舆论监督司法是保障司法公正的重要手段,公开监督、全程监督和多维监督等是其有效模式。最大限度地发挥舆论监督的积极作用,可以促使司法权力沿着正确的轨道运行,保证社会正义的实现,加速公共权力的制度建设。     

新公共管理理论对我国建设服务型政府的启示

新公共管理理论是西方国家在特定的政治、经济和科学技术发展下的政府管理改革的必然产物,它在一定程度上反映了西方公共行政改革的发展态势,也在一定程度上促进了西方各国经济和社会的发展,在缓解经济和政治危机方面取得了巨大成效。目前,我国正在进行服务型政府建设,因此,有必要借鉴西方新公共管理的某些理论来推动我国的服务型政府建设。     

论加强公法整体研究的现实意义

由于公法所具有的强烈时代性与地域性以及公法所涉及问题的复杂性,决定了公法研究必须带着问题意识,就公法建设中存在的矛盾和困难有针对性地加强系统性的整体研究。本文认为,就目前中国现实国情而言,加强公法整体研究的意义集中体现于四个方面,即政治意义在于促进宪政建设,法治意义在于尊重和保障人权,社会意义在于推进制度反腐败建设,学术意义在于促进公法规范协调发展。而无论是宪政建设、尊重和保障人权以及制度反腐败的实现,必须依赖于一国公法制度规范的总体设计和协调整合,这正是加强公法整体研究学术价值之所在。     

挤压家兔后肢导致急性肝损伤和抗氧化能力降低

目的本实验观察挤压家兔后肢导致肝氧化损伤,探讨氧化应激在挤压家兔后肢导致肝损伤中的作用。方法用标准重物间断压迫家兔后肢复制模型。采用生化方法检测ALT和AST活性;分析天平称重方法检测肝脏湿干重比值(W/D);比色法测定肝组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性和总抗氧化能力(T-AOC)以及丙二醛(MDA)含量变化;并观察肝组织病理学改变。结果血清ALT和AST活性明显增高(P<0.01),肝细胞明显肿胀,肝窦轻度淤血,而肝脏湿干重比值(W/D)轻度增加(P<0.05);肝脏氧化损伤明显加重,MDA含量增高,而肝脏抗氧化能力下降,SOD、CAT、GSH-Px活性和T-AOC分别比对照组降低了17%、29%、24%和21%(P均<0.01)。结论挤压家兔后肢引起急性肝损伤及肝脏的抗氧化能力下降;肝脏局部抗氧化能力下降可能是肝损伤的重要因素。     

Application of HLA-DRB1 genotyping by oligonucleotide micro-array technology in forensic medicine

The human leukocyte antigen (HLA) system is known to be the most complex polymorphic system in the human genome. Among all of the HLA loci, HLA-DRB1 has the second largest number of alleles. The purpose of this study is to develop an oligonucleotide micro-array based HLA-DRB1 typing system for use in forensic identification, anthropology, tissue transplantation, and other genetic research fields. The system was developed by analyzing the HLA-DRB1 (DRB1) genotypes in 1198 unrelated healthy Chinese Han individuals originating from various parts of China and residing in Shanghai, China. METHOD: Polymerase chain reaction (PCR) coupled with the oligonucleotide micro-array technology was used to detect and type HLA-DRB1 alleles of the sample individuals. The reliability, sensitivity, consistency and specificity were evaluated for use in forensic identification. Furthermore, a meta-analysis was carried out by comparing the allele frequencies of the HLA-DRB1 locus with those of other Chinese Han groups, Chinese minorities and other ethnic populations. RESULTS: All the DNA samples yielded a 273 bp amplification product, with no other amplification products in this length range. The minimum quantity of DNA detected by this method is 15 ng in a PCR reaction system of 25 microl. The population studied appeared to be not in Hardy-Weinberg equilibrium. Observed heterozygosity (Ho), expected heterozygosity (He), expected probability of exclusion (PE), polymorphic information content (PIC), and discrimination power (DP) of the HLA-DRB1 locus from the Shanghai Han ethnic group were evaluated to be 0.8022, 0.8870, 0.7741, 0.8771, 0.9750, respectively. A total of 25 HLA-DRB1 alleles were identified. HLA-DRB1*09XX, *04XX, *12XX and *15XX were the most frequent DRB1 alleles, which were observed in 58.76% of the sample. One hundred and sixteen genotypes were found. The five most frequent genotypes were: *04XX/*04XX (0.0626), *09XX/*09XX (0.0593), *04XX/*09XX (0.0551), *09XX/*15XX (0.0384) and *08XX/*12XX (0.0351). The meta-analysis showed that there were uniquely distributed features of DRB1 alleles among various ethnic populations and among the studied population groups from various regions with the same ethnic origin. CONCLUSIONS: An HLA-DRB1 genotyping system has been developed and established based on the oligonucleotide micro-array technology. The HLA-DRB1 typing of the Han population in Shanghai has revealed a relatively high heterogeneity. Information obtained in this study will be useful for medical and forensic applications as well as in anthropology research. Large-scale micro-array detection is highly accurate and reliable for DNA-based HLA-DRB1 genotyping. These results suggest that HLA-DRB1 DNA polymorphisms and the database of the Shanghai Han group have useful applications in processing forensic casework (as personal identification, paternity test), tracing population migration and genetic diagnosis.     

小鼠皮肤切创愈合过程中iNOS和eNOS表达的免疫组织化学研究

目的观察小鼠皮肤切创愈合过程中,iNOS和eNOS在损伤区及损伤周边区内的表达及其变化规律。方法小鼠背部制作全层切创,应用免疫组织化学技术观察伤后切创组织中iNOS和eNOS的表达,并和无切创的小鼠作为对照。结果损伤区及损伤周边区细胞,伤后3h的损伤皮肤组织中可见少量的多型核细胞表达iNOS和eNOS,伤后6～24h,大部分浸润的中性粒细胞和单核细胞为iNOS和eNOS阳性。随着时间的延长,iNOS和eNOS阳性细胞以单核细胞和成纤维细胞为主。伤后3hiNOS的阳性细胞比率较低,6h～1d持续性增加并于1d达到最高峰,3～7d维持在一个相对稳定的水平直至伤后10d再次达高峰,10～14d开始下降。eNOS的阳性细胞率在伤后1～3h表达较低,6h～3d持续性增高,并在3d达到最高峰,在其后的5d内保持稳定表达,之后开始下降。而且损伤区及损伤周边区、特别是肉芽组织内的新生血管可见iNOS和eNOS不同强度的表达。结论小鼠皮肤切创愈合过程中,iNOS和eNOS在损伤周边区内多型核细胞、单核细胞和成纤维细胞中表达,其时序性变化可望用于皮肤损伤时间的推断。     

论抗战时期桂林人口伤亡

日军的侵略给桂林带来了巨大的灾难。整个抗战时期,有确凿数据或事实作为依据的官兵阵亡人数 为6729人。根据战争损失专家提出的比例(即负伤与死亡的比例约为2:1)计算,则抗战中桂林籍伤亡的官兵和在 桂林伤亡的其他省籍官兵的人数约为20000人左右。而民众死亡的数量根据人口的发展情况判断应在《广西年鉴》 所记载的数字的基础上再加上1万人,即约为3万人。