Acidic and neutral drugs screen in blood with quantitation using microbore high-performance liquid chromatography–diode array detection and capillary gas chromatography–flame ionization detection

Blood previously acidified with aqueous saturated ammonium chloride solution was extracted with ethyl acetate. The dried extract was subjected to acetonitrile–hexane partition. The acetonitrile portion was analysed for the presence of acidic and neutral drugs by HPLC–DAD (200 mm×2.1 mm I.D. microbore ODS-Hypersil column) and GC–FID (25 m narrow-bore×0.25 mm I.D. HP-5 column with 0.33 μm film thickness). The protocol was found to be suitable for both clinical toxicology (including emergency toxicology) and postmortem toxicology. At least 66 drugs of interest were unequivocally identified by RRTs (HPLC) and UV spectra (DAD) match while another 12 were unequivocally identified by double RRTs match (HPLC and GC). Quantitation was facilitated by incorporating calibration blood standards in each assay batch. The five drugs most commonly encountered in clinical blood specimens (1150 cases) were: paracetamol (47.4% of the cases); chlormezanone (6.6%), theophylline (1.74%), naproxen (1.65%) and mefenamic acid (1.56%). The following drugs were detected in toxicologically significant quantities in postmortem blood specimens (245 cases): phenobarbitone (1.22% of the cases), naproxen (0.82%), chlormezanone (0.82%), theophylline (0.82%), carbamazepine (0.41%) and paracetamol (0.41%).     

浅谈公安干部竞聘机制的完善

公安干部队伍建设是搞好公安工作的前提和保证。公安干部竞聘机制是公安人事制度改革的方向之一,但在实际运作中还存在不少问题。公安干部的竞聘必须遵循一定的原则,把握一些重要环节,正确处理好三对关系。     

浅析网络著作权问题

网络越来越深入人们的生活,给人们带来方便的同时也出现很多问题,其中包括网络著作权的侵权问题。作者从网络著作权、网络著作权侵权及法律保护问题等方面提出了自己的看法。     

东莞模式:中国经济走向世界的成功尝试

东莞模式是对东莞经济发展道路的一种概括,主要指东莞充分发挥人缘、地缘、政策优势,以加工贸易为突破口,以外向型经济为主导,迅速实现农村工业化、产业现代化、经济国际化的经济发展模式.这是一种三赢模式,它不公使东莞赢得了在国际经济中的一席之地,而且使国际资本寻找到了一个低风险、高回报、较稳定的投资场所,同时,更为重要的,这是中国经济走向世界的成功尝试.东莞模式为我国加入WTO中国经济大规模地融入国际经济提供了许多有益的经验.     

Enzyme activity of PGM1 in erythrocytes

The enzyme activity of phosphoglucomutase (PGM) has been investigated in red cell haemolysates from 142 individuals and compared to the sub-type as determined by isoelectric focusing. The 10 phenotypes showed significant differences in PGM-activity which indicates that there is a correlation between the level of activity and the isolelectric point of homozygotes. No indication of silent alleles or alleles with reduced activity was found in this collective.     

Recovery of decomposed and skeletal human remains in the "Green River Murder" Investigation. Implications for medical examiner/coroner and police

The Green River Murder Investigation in King County, Washington, is currently the longest active serial murder investigation in U.S. history. During its course, over 26 separate scenes with from one to five victims each have been processed. The experience of the authors is presented in order to acquaint other agencies with techniques of outdoor scene processing that have evolved during recovery of remains from Green River and other skeletal cases.     

法学研究的另一思路

<正> 向市场经济转型的目标正式确立,似乎再一次给了法学为自己定位和重新发现自身价值的机会。随“市场经济是法制经济”这一命题的提出,法学界显得相当活跃和颇有生机。从围绕这个命题而发表的许多文章来看,法学家们主要做的是两个方面的工作:一方面是为法学引入了许多以前可能被排斥或被忽视的新观念、新价值;另一方面,则再次强调了加强立法、严格执法的法治原则。值得注意的是,就后一个方面而言,我们目前的一般理论水平与十多年前经过“要人治还是要法治”的讨论所达到的认识相比,似乎并无很大的进展。法学研究现在面临的重     

从“阳春白雪“到“下里巴人“——“易中天现象“析

"易中天现象"的核心是学术大众化的问题,而学术大众化的核心则是以什么方式、内容、目的走向大众的问题.从"易中天现象"中我们发现,学术要走向大众,必须具有大众化的传播和阐释方式、大众化的现实诉求和精神狂欢、大众化的价值多元和"海纳百川".具体到易中天讲座,则主要在于如何把握历史.易中天是通过灵动语言的当代阐释和以小寓大的文化批判来把握三国历史的.这是"易中天现象"火爆的真正原因和价值.