SELEX技术筛选甲基苯丙胺适配子

目的利用SELEX筛选甲基苯丙胺适配子,并初步鉴定获得甲基苯丙胺的适配子。方法体外合成全长为76 bp的随机ssDNA文库,以甲基苯丙胺完全抗原偶联到溴化氰活化琼脂糖上,作为固相靶分子对随机ssDNA文库进行SELEX筛选。利用SPR测定适配子和甲基苯丙胺的结合率和特异性,通过MFOLD分析软件对适配子进行二级结构预测和结合位点分析。结果经过10轮筛选后,甲基苯丙胺适配子的结合率由88.3 RU上升到113.7 RU,表明特异性适配子得到逐步富集。获得的适配子进行测序,得10个序列,二级结构均为茎-环结构,可能是适配子和甲基苯丙胺结合的结构基础。结论本研究使用SELEX技术筛选小分子毒品甲基苯丙胺适配子的方法,获得与甲基苯丙胺特异性结合的适配子序列,揭示了适配子与甲基苯丙胺结合的基本结构,为制备特异性甲基苯丙胺检测试剂盒奠定了基础。     

金炳镐和当今中国的马克思主义民族理论研究--兼论中国民族理论学科发展和学科体系建设

金炳镐是当今中国马克思主义民族理论研究领域的开拓者和领军人物之一.他在诸多领域都取得了卓越的成就,最主要的是在对马克思主义民族理论和当代中国民族问题的研究方面,表现出了总结性、成熟性和创新性三个特点.其著作<民族理论通论>在马克思主义民族理论学科体系中居于核心地位.     

Sustained Development Leads to Higher Living Standards简

正CHINA’s economy has significantly advanced over the past 35 years of reform and openingup.Chinese people’s incomes have risen and their living standards have improved,from barely having enough to eat and adequate clothing to today’s generally better-off lifestyle.     

The Ascent Of Haba Snow Mountain

正IN 1995,a group of Chinese mountaineers reached the summit of Haba Snow Mountain,5,396 meters above sea level,making it the highest snow peak in Yunnan Province to be conquered by humans.The mountain,in the eyes of professional mountaineers,is"entry level."However,to amateurs,it is a game for the brave.At 10 a.m.on October 5,2013,I,along with 17teammates and a group coordinator,reached the top of Haba.Standing next to the altitude mark,I texted"I made it!"to my family.The message was simple but we all knew it was hard earned.     

Tax Reforms under the Principle of Transparency,Efficiency and Equity

正CHINA has been comprehensively deepening reforms since the convening last November of the Third Plenary Session of the 18th CPC Central Committee.Tax reforms affect the interests of each citizen and every enterprise,and are inextricable from economic development and social stability.As one of the world’s main emerging economies,China operates a fiscal system under the principle of high fiscal revenues and high fiscal expenditure,thus ensuring     

犯罪成立的法律标准之反思与重构-基于对情节要素的独立评价

认定是否成立具体的犯罪是定罪活动的核心任务。传统刑法理论关于“犯罪构成是犯罪成立的充分条件”的命题,在犯罪概念与犯罪构成、定性因素与定量因素之间关系的处理上存在着“剪不断理还乱”的悖理与纠葛。合理解决此等困扰,只有突破犯罪成立标准的传统认识,将集中体现行为社会危害性程度的概括性情节因素置于犯罪构成之外独立评价,进而重新构建犯罪成立的法律标准,即“犯罪构成＋情节非显著轻微=犯罪成立”。     

Strong Growth Prospects for the Chinese Economy

FIVE years after the onset of the global financial crisis the world economy is finally revving up - a recovery led by emerging economies that include China. Tables 1 and 2 below show that, despite a slowdown compared with last year, the year-on-year and quarter-to-quarter growth rate of the Chinese economy still outpaces that of the U.S.     

集体土地征收中的主要程序问题研究

集体土地征收中公共利益和补偿标准的开放性和动态性,强化了征地对程序正义性的依赖性。集体土地征收应当顺应参与式行政的发展趋势,进一步强调程序参与和程序平等原则,保障被征地人提前、全面和深度介入土地征收,强化其征收话语权,保障其土地财产权。从确定土地利用规划方案、认定公共利益、设计补偿安置方案、分配土地补偿款项、争议解决程序和司法救济机制等各个环节,都应当拓宽被征地人畅通、平等的参与渠道。     

被害人责任法定化初探

被害互动理论揭示了被害人在犯罪进程中承担着事实责任,这种责任经规范评价后应该成为影响犯罪人定罪量刑的情节。司法实践不能承受之弊端呼唤着这一情节的法定化,理论和现行立法为其法定化提供了丰富的既有资源。被害人责任法定化应该走一条以刑法分则为基础,以刑法总则为总纲,以司法解释作为保障的渐进之路。