小反刍兽疫病毒H糖蛋白基因原核表达载体的构建及表达

参照GenBank中小反刍兽疫病毒(PPRV)H抗原基因序列,人工合成了PPRV H基因,将其克隆至pUC18-T质粒中,转化E.coliJM109感受态细胞,构建并选择PPRV H基因克隆重组质粒,经核苷酸序列分析正确,将其克隆至pBAD/Thio-TOPO载体中,转化E.coliTOP10感受态细胞,核苷酸序列分析证实,成功构建了PPRV H基因重组表达载体。经不同浓度L-阿拉伯糖诱导,可稳定、高效地表达PPRV H抗原。SDS-PAGE分析结果表明,用终浓度为0.2 g/L的L-阿拉伯糖诱导5 h的表达量最高,表达蛋白为分子质量约83 ku的融合蛋白;经薄层扫描分析,其表达产量约占菌体总蛋白的10%。Western-blotting检测表明,诱导的蛋白能与PPRV H蛋白单抗发生特异性反应,说明表达的融合蛋白中含有PPRV H糖蛋白抗原。     

准中止犯概念及在我国刑法中的倡导

准中止是不具备中止成立的条件犯罪行为,但却按照中止处罚的情形。在实践中,准中止一般包括两种情形:一是由被害人或第三人阻断因果关系而形成的准中止;另一是由犯罪行为的性质阻断因果关系而形成的准中止。准中止犯理论符合我国中止犯存在的理论基础,也与我国刑法的主客观相统一原则内涵契合,它有利于减少刑法适用上的冲突,从而实现我国的刑罚目的。因此,我国刑法理论宜倡导准中止犯的概念并在立法中加以体现。     

The Earthquake Will Not Change the Momentum of China＇s National Economy

THE world＇s attention has been riveted by the devastating earthquake that hit Sichuan Province in May. Apart from timely relief assistance, international think tanks and financial organizations have also been analyzing its impact on the Chinese economy. One of the world＇s leading companies for catastrophe risk estimation, Air Worldwide, has stated that the economic loss caused by the earthquake may reach US $20 billion. However, the Financial Times reports that the disaster will not badly impact China＇s economy overall, despite the tragic losses, although analysts at JP Morgan believe that the temporary disorder may result in a new rise in consumer prices.     

地方政府绩效评价技术路径的比较分析

层次分析法是政府绩效评估的常用方法,但应用于第三方评价中存在技术性障碍.为此,华南理工大学课题组在实证研究中对层次分析法进行了改良,构建了作为层次分析法特例的技术路经.改良结果表明,层次分析法特例模型较之简单采用层次分析法得出的结果更具客观性和全面性.     

论我国地方人大行使监督权存在的问题及建议

我国地方人大行使监督权缺乏具体操作的法律依据,各种关系没有理清,地方人大的地位在实际中没有得到真正的确立。要更新观念,理顺关系,确立地方人大行使监督权核心的地位,进一步制定、完善地方人大行使监督权的法律法规,进一步制定、完善地方人大行使监督权的法律法规。代表应具有较高的素质,充分发挥我国监督体系中各种法律监督形成的合力作用,进一步加强我国的监督法制建设,充分行使地方人大监督职能。     

The Arab Spring and the Chinese Autumn

For 500 years the West was on the rise, culminating in Globalizaiton 1.0—the open system of trade, information flows and the spread of technology on the terms and in the image of the West. The benefits of that system over the last 30 years have led to the rise of the emerging economies. As a result we are entering the new era of Globalization 2.0 characterized by new forms of non‐Western modernity and the interdependence of plural identities. The advent of this new era has been hastened by the fiscal and financial crisis in Europe and the United States. Turkey, with its Islamic‐oriented democracy that has become a template for the liberated peoples of the Arab Spring, and China, with its effective neo‐Confucian form of governance, are the most sharply defined new players in this multi‐polar and multi‐dimensional world. In this section, one of Turkey's most insightful sociologists examines the post‐secular transformation of that nation. One of China's more provocative philosophers proposes a hybrid model that combines what has been learned from the experience of Western and Chinese governance in a way that “enhances democracy” in both systems.     

毛泽东外交战略的国内根源——官僚政治与群众路线的两难选择

毛泽东,作为新中国第一代领导人,其外交战略在很大程度上反映了中国的传统和现实,同时也为今天的中国外交留下了很多启示与教训。在改革开放的今天,中国与世界的关系日趋密切,中国的国际地位有了很大提高。回顾老一辈革命家的外交战略和外交经验,对我们理解今天中国外交的国内国际基础,及其未来发展,具有深远的意义。本文主要是以毛泽东在中国革命和现代化建设的国内经验中存在的官僚政治与群众运动的两难选择,来分析各个时期两者关系是如何影响毛泽东的外交战略的。希望本文能够为围绕毛泽东外交战略中的得与失的争议,提供一个将国内政治与国际政治结合的新视角。     

无形资产的管理策略

本文主要对无形资产管理中的专利管理策略,商标管理策略和商业秘密管理策略进行了探讨。     

Transformations of China’s Legal System

FROM"rule of man"to"building a legal system,"to"rule of law"and finally,to the"rule of the Constitution,"the development of China’s legal structure epitomizes its learning curve of state governance.From Rule of Man to Building a Legal System The fi rst building block of the PRC’s legal construction was the establishment of the socialist legal system.In September 1949,the Common Program of the     

目前诉讼调解应注意的若干问题

诉讼调解充分尊重当事人的意思自治,有利于其服判息诉、履行义务并最终达到定纷止争、社会和谐的司法理想。目前,在审判实践中关于诉讼调解工作应关注"五项注意"。