Characterization and haplotype analysis of 10 novel Y-STR loci in Chinese Han population

In this study, we analyzed allelic sequences of 10 novel Y-specific STR loci, DYS454, DYS510, DYS513, DYS520, DYS542, DYS544, DYS552, DYS561, DYS587 and DYS593, surveyed the distribution of haplotypes in a Chinese Han population. Extracted DNA was amplified with PCR, followed by a horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system. Purified alleles were sequenced on DNA sequencer (ABI Model 377) to verify the number of motif repeats. The number of alleles observed at each locus ranged from 3 to 8, yielding 102 haplotypes in 103 unrelated males samples. The allele diversity values for each locus ranged from 0.2099 (DYS544) to 0.7523 (DYS552). The haplotype diversity using all these loci was 0.9998. Our study revealed that they were valuable Y-specific markers for forensic applications.     

中国特色妇女理论的后现代主义方法研究

合法性问题是中国特色妇女理论方法研究的一个重要方面,在一定意义上说解决了合法性问题也就是解决了方法问题。中国特色妇女理论的构建在方法上首先须做到“三个一致”;其次要了解后现代语境中的合法性与非合法性问题,破除对理性主义和科学主义的盲从,倡导妇女理论中的个性化、多元化表达;再次,吸取合法性即“悖谬”的启发,制定和遵从中国特色妇女理论自身的言说规则。     

浅析青少年的孤独感及其影响因素

孤独感是一种主观的心理感受或体验,是影响青少年健康发展的重要因素。青少年的孤独感与同伴关系、人格因素、社会支持及归因倾向密切相关。分析影响青少年孤独感的因素,采取有效的应对措施,减少孤独感是素质教育的重要课题之一。