新时期公安工作贯彻群众路线的思考

群众路线是公安工作的生命线和根本路线,公安机关各级领导干部要主动适应当前形势的新变化,牢牢把握人民群众的新期待,积极探索新形势下专门工作与群众路线相结合的新办法,着力提高广大民警做好群众工作的本领,不断密切同人民群众的血肉联系,努力为公安工作赢得最广泛、最可靠、最牢固的群众基础。     

A Simple Method of VNTR D1S80 Locus Allelic Ladder Construction for Capillary Electrophoresis‐based Genotyping

VNTR D1S80 locus genotyping has been largely replaced in forensics by STR. As the statute of limitations on murder cases was abolished in the Republic of Korea in July 2015, the demand for re‐analysis of DNA from unresolved murder cases has increased. The National Forensic Service includes several recorded D1S80 genotypes as crucial clues. Here, we re‐established the D1S80 analysis system using capillary electrophoresis and confirmed the reproducibility of the system by comparison with the genotypes of eight DNA samples that had been analyzed using PAGE in 2006. In addition, we created an allelic ladder via new methodology using flanking region sequences. A single DNA sample (K562) and seven primers were used for the new ladder, which contains 12 alleles. Although artificial owing to the use of the flanking region rather than repeat unit reduction, the method is rapid and simple, and could be applicable in any laboratory.