基于分子克隆方法制备标准分子量片段混合物

目的制备标准分子量DNA片段混合物。方法选取pMD18-T载体部分序列作为插入片段,设计引物,制备包含不同大小的标准分子量片段的克隆。大量培养细菌提取质粒,用双酶切、连接荧光接头的方法制备不同大小的带有荧光的标准分子量片段,混合,纯化,最终获得标准分子量片段混合物(内标)。结果用分子克隆方法制备出具有实用性的标准分子量片段混合物,其单个标准分子量片段大小分别为80bp、124bp、194bp、224bp、254bp、304bp、349bp、399bp、424bp、454bp。并且这些标准分子量片段混合物可以准确地对DNATyper15试剂盒的扩增产物进行检测。结论应用该方法制备了能满足研究和实验室要求的标准分子量片段混合物,为DNA分子量标准物标准品的制作提供了一种新的方法。     

论中东国家清真寺的教育功能

起步于清真寺的伊斯兰教育,历经讲经布道——库塔布——学习圈——麦德莱赛——寺校合一模式的大体演变过程,折射出伊斯兰的基本教育观,并凸显中东国家清真寺的教育功能;在长期的历史发展中,中东清真寺教育形成了神圣性、慈善性、两世性、民间性等基本特性,并产生了深远影响;伊斯兰教育应继续大力弘扬伊斯兰教的和平本质,积极探索实现伊斯兰教育与国民教育职能剥离的可行途径,在最大程度地满足受教者的宗教诉求与社会诉求中实现伊斯兰教育的公益目标。积极构建对话文明,也是伊斯兰教育公益事业的当务之急。     

The Energy Cooperation between China and India in the Post-crisis Era

In recent years, both China and India have paid great attention to the energy security issue. As they are both big energy consumers and importers, launching energy cooperation between China and India is mutually beneficial. Cooperation between the two countries should stick to the following principles： strengthening communication; avoid unnecessary conflict; advancing technical exchange and jointly promoting the stability of the world energy market.     

Thoughts on Pushing Forward People-to-People Diplomacy in the New Era

<正>I.The Situation of China's People-to-People Diplomacy in the New Era As an indispensable part in China's overall diplomacy, the people-to-people diplomacy aims at enhancing the friendship between peoples, and hence promoting state-to-state relations. As one of the most vibrant areas with full development potential in China's overall diplomacy,     

My First Visit to Japan

<正>I made my first visit to Japan with the delegation of the Chinese Association for International Understanding (CAFIU) from March 27 to 30, 2013. Headed by Secretary-General Ni Jian, the five-member delegation visited Osaka, Kyoto and Tokyo. In fact, I had mixed feelings before the visit: as the most developed country in Asia,     

Babycare’s International Cooperation in Early Childhood English Teaching

Established in 1998, Babycare Consulting Co. Ltd. (hereinafter referred to as Babycare) is a new-type enterprise offering early education to babies and children aged 0-6. In accordance with the law of physical and mental development of babies and children, the company develops courses such as healthy pregnancy     

Decomposition of embodied exergy flows in manufactured products and implications for carbon tariff policies

This article uses the concept of embodied exergy as metrics in designing incentive policy instruments to tackle the inefficiency of energy operations. Based on the second law of thermodynamics and energy’s economic properties as both a private commodity and a public good, it maintains that energy can be measured by separating the useful exergy embodied in a manufactured product from its waste exergy (anergy) as emissions and sunk wastes in a production process. It is rational to benchmark the content of useful exergy embodied in products for any incentive policy design to encourage green production. This article uses trade data between China, Japan and the EU countries to compare the embodied exergy and waste exergy embodied in traded manufactured products. It proposes using a negative value-added tax as an incentive instrument instead of full-scale carbon tariffs to encourage green production and to fence against carbon evasion behaviour.     

中国外交向立体化格局的全面发展

随着经济的发展、综合国力的增强和国际影响的扩大,中国外交正以崭新的姿态出现在国际舞台上,向着全方位、多层次、宽领域的立体化方向演进,取得了辉煌的成就。     

Enhance Under standing and Cooperation to Builda Bridge of China-Hungary Friendship——Hungarian World Bridge FoundationDelegation Visited China

At the invitation of the Chinese Association for International Understanding (CAFIU), Deputy Speaker of the Hungarian National Assembly and Power Equipments Co., Ltd and Huawei Technologies Co., Ltd, and attended the opening ceremony of the 8th China (Shenzhen) International     

The World and Me

At the invitation of Mr. Nakata Sen, Chief Director of the Japanese special non-profit organization The World, CAFIU’s Secretary- General Ni Jian and I visited Japan and attended the 10 th annual conference of The World from 20 th to 24 th August. It was my third visit to