固相萃取──紫外导数光谱法测定脏器中敌鼠钠含量

肝、肾、肺、脑等脏器中敌鼠钠含量的题示测定法，使用国产GDX403树脂为革取材料，溶剂消耗少，操作简便，回收率约70％，检测下限小于1μg．g-1，适合做为法医毒物分析常规方法。     

对我国在建设责任政府过程中加强居安思危未雨绸缪能力建设的思考

建设责任政府是我国转变政府职能的重要内容,而要建立责任政府,就不能仅仅关注于对问题的解决及出现问题后的责任追究,它还必须更关注于对问题的预防,对潜在危机的消除。这就必须加强政府居安思危、未雨绸缪的能力建设。为此,政府不仅要提高领导者的相关素质,更重要的是要创新体制和机制,要在建立有效的政策监督、评估机制、建立权责明确的组织体系、加强政策研究机构建设等方面加大力度。     

中华民族精神与和谐社会建设问题研究

深入研究和探讨中华民族精神与和谐社会建设的关系,大力弘扬中华民族精神,为构建社会主义和谐社会服务,是新世纪新阶段党的民族工作必须关注的重要问题之一.     

网络传播伦理原则简论

网络传播的飞速发展给传播伦理带来了巨大的冲击.如何充分认识网络传播的特有规律,建构网络传播中的伦理规范,已成为我国网络传播研究的主要问题.网络环境下媒体伦理的建设要通过社会和媒体及广大网民的共同努力、外部强制和个人自律、道德引导和法律控制的多方位多层次的建构策略,才能建立和完善网络传播伦理,使网络传播良性有效地运行.     

Application of HLA-DRB1 genotyping by oligonucleotide micro-array technology in forensic medicine

The human leukocyte antigen (HLA) system is known to be the most complex polymorphic system in the human genome. Among all of the HLA loci, HLA-DRB1 has the second largest number of alleles. The purpose of this study is to develop an oligonucleotide micro-array based HLA-DRB1 typing system for use in forensic identification, anthropology, tissue transplantation, and other genetic research fields. The system was developed by analyzing the HLA-DRB1 (DRB1) genotypes in 1198 unrelated healthy Chinese Han individuals originating from various parts of China and residing in Shanghai, China. METHOD: Polymerase chain reaction (PCR) coupled with the oligonucleotide micro-array technology was used to detect and type HLA-DRB1 alleles of the sample individuals. The reliability, sensitivity, consistency and specificity were evaluated for use in forensic identification. Furthermore, a meta-analysis was carried out by comparing the allele frequencies of the HLA-DRB1 locus with those of other Chinese Han groups, Chinese minorities and other ethnic populations. RESULTS: All the DNA samples yielded a 273 bp amplification product, with no other amplification products in this length range. The minimum quantity of DNA detected by this method is 15 ng in a PCR reaction system of 25 microl. The population studied appeared to be not in Hardy-Weinberg equilibrium. Observed heterozygosity (Ho), expected heterozygosity (He), expected probability of exclusion (PE), polymorphic information content (PIC), and discrimination power (DP) of the HLA-DRB1 locus from the Shanghai Han ethnic group were evaluated to be 0.8022, 0.8870, 0.7741, 0.8771, 0.9750, respectively. A total of 25 HLA-DRB1 alleles were identified. HLA-DRB1*09XX, *04XX, *12XX and *15XX were the most frequent DRB1 alleles, which were observed in 58.76% of the sample. One hundred and sixteen genotypes were found. The five most frequent genotypes were: *04XX/*04XX (0.0626), *09XX/*09XX (0.0593), *04XX/*09XX (0.0551), *09XX/*15XX (0.0384) and *08XX/*12XX (0.0351). The meta-analysis showed that there were uniquely distributed features of DRB1 alleles among various ethnic populations and among the studied population groups from various regions with the same ethnic origin. CONCLUSIONS: An HLA-DRB1 genotyping system has been developed and established based on the oligonucleotide micro-array technology. The HLA-DRB1 typing of the Han population in Shanghai has revealed a relatively high heterogeneity. Information obtained in this study will be useful for medical and forensic applications as well as in anthropology research. Large-scale micro-array detection is highly accurate and reliable for DNA-based HLA-DRB1 genotyping. These results suggest that HLA-DRB1 DNA polymorphisms and the database of the Shanghai Han group have useful applications in processing forensic casework (as personal identification, paternity test), tracing population migration and genetic diagnosis.     

浅析赌博罪认定中的几个疑难问题

赌博罪,一般是指以营利为目的,聚众赌博、开设赌场或以赌博为业的行为。在司法实践中,认定与处理赌博犯罪案件常常会出现一些疑难问题,尤其是随着网络赌博犯罪的出现,关于赌博罪的认定,赌博罪共犯的认定,赌博活动中涉及其他犯罪的处理等问题疑难较多,只按照我国《刑法》的具体规定是远远不够的,应参照2005年最高人民法院、最高人民检察院发布的《关于办理赌博刑事案件具体应用法律若干问题的解释》执行,以更有效地打击和遏制赌博犯罪。     

骑跨伤在机动车碰撞自行车事故中的特征及生物力学分析

目的以真实案例为基础,研究自行车与机动车碰撞交通事故中,不同机动车型、交通方式等因子造成自行车骑车人下肢内侧骑跨伤的特征,分析其生物力学机制。方法选取上海地区发生的自行车与机动车碰撞交通事故案例140例,全面收集现场、自行车、机动车、人体、交通方向等信息,运用回顾性研究方法分析,同时,选取其中典型案例,通过计算机仿真技术模拟事故发生过程,提取人体各受伤部位的动力学响应数值,如加速度、力和力矩等,与案例研究结果对比。结果全部事故中出现骑跨伤与无骑跨伤案例频数相等;侧面撞击与前后方向撞击骑跨伤的发生情况无显著性差别;大型车辆撞击事故中出现骑跨伤频率较低;骑跨伤的发生频率随车速加大而增高;就骑跨伤的分布来说,撞击侧下肢骑跨伤位置低(链条、脚蹬管等损伤),而对侧骑跨伤位置高(鞍座损伤),在小型车辆撞击时该特点更明显。结论分析自行车骑车人的骑跨伤特征和生物力学机制有望对道路交通行为方式鉴定提供新依据。     

商业银行并购的法律性质及其制度完善

商业银行并购行为具有多重法律性质,对其进行调整的法律体系应当体现意思自治、效率、反垄断等基本原则。我国现有商业银行并购法律未能严格遵循以上原则且缺乏系统性。有必要在准确把握银行并购法律性质的基础上制定《商业银行并购条例》,完善银行并购民事合同制度、申报审批以及反垄断制度等,从而有效地维护我国银行业金融体系安全和金融市场发展。     

从“发展主义”到“以人为本”——和谐社会建设下中国社会转型的新视角

发展主义对改革开放以来的中国社会转型产生了巨大影响,但是由于存在着理论与实践的二元悖反,发展主义也带来了社会转型各领域的失衡。在这种形势下,贯彻以人为本的发展理念,实现全面、协调、可持续的发展,寻求人与自然的和谐,以建设社会主义和谐社会来推进当前中国的社会转型。     

粤港澳大湾区跨区域基础设施协调制度的构建与完善--基于港珠澳大桥和欧盟TEN-T项目的比较

跨区域基础设施项目的建设、运营和管理是区域治理的重要内容之一,往往面临集体行动困境。因此,需要建立区域治理的协调制度来协调多元治理主体、项目建设和不同利益群体这三个层面来克服集体行动困境,共同提供跨区域的公共产品和服务。欧盟和粤港澳大湾区在各自的跨区域基础设施项目中建立起了各具特色的区域协调制度。未来粤港澳大湾区跨区域基础设施的治理可以借鉴欧盟的经验,在法治化的框架下实施区域协调;完善中央层面、区域层面和地方层面的多元治理主体间的权能分配制度;区域协调机构应着重提升"跨区域"的公共利益;扩大区域治理的协调范围,创新公众参与机制。