Comparison of feature selection and data fusion of Fourier transform infrared and Raman spectroscopy for identifying watercolor ink

The identification of different kinds of watercolor inks is an important work in the field of forensic science. Four different kinds of watercolor ink Spectroscopy data fusion strategies (Fourier Transform Infrared spectroscopy and Raman spectroscopy) combined with a non-linear classification model (Extreme Learning Machine) were used to identify the brand of watercolor inks. The study chose Competitive Adaptive Reweighted Sampling (CARS), Random Frog (RF), Variable Combination Population Analysis-Genetic Algorithm (VCPA-GA), and Variable Combination Population Analysis-Iteratively Retains Informative Variables (VCPA-IRIV) to extract characteristic variables for mid-level data fusion. The Cuckoo Search (CS) algorithm is used to optimize the extreme learning machine classification model. The results showed that the classification capacity of the mid-level fusion spectra model was more satisfactory than that of single Infrared spectroscopy or Raman spectroscopy. The CS-ELM models based on infrared spectroscopy used to recognize the watercolor ink according to brands (ZHENCAI, DELI, CHENGUANG, and STAEDTLER) obtained an accuracy of 66.67% in the test set using all spectral datasets. The accuracy of CS-ELM models based on Raman spectroscopy was 67.39%. The characteristic wavelength selection algorithms effectively improved the accuracy of the CS-ELM models. The classification accuracy of the mid-level spectroscopy fusion model combined with the VCPA-IRIV algorithm was 100%. The data fusion method increased effectively spectral information. The method could satisfactorily identify different brands of watercolor inks and support the preservation of artifacts, paintings, and forensic document examination.     

弓形虫烯醇化酶2基因的原核表达与鉴定

为深入研究弓形虫烯醇化酶的生物学功能,根据已发表的eno2基因序列设计引物,通过RTPCR方法扩增弓形虫RH株的eno2基因。将eno2克隆到载体pET-28a(+)中后,重组质粒转化大肠杆菌BL21(DE3)感受态细胞,IPTG诱导表达并优化表达条件,SDS-PAGE分析表达情况,并通过Western-blot和间接免疫荧光试验(IFA)鉴定其免疫活性。结果显示,eno2基因全长1 353bp,编码444个氨基酸,与GenBank中登录的弓形虫烯醇化酶基因的核苷酸序列及其推导的氨基酸序列的同源性均高达99.8%,与其他物种的烯醇化酶的同源性较低。在pH7.5、28℃和IPTG终浓度0.1mmol/L条件下,重组蛋白ENO2得到高效可溶性表达,分子质量约为55ku。Western-blot结果显示,重组蛋白能被感染弓形虫的犬阳性血清识别,说明ENO2具有较好的反应原性,可以作为血清学诊断抗原。IFA表明,NO2在弓形虫的细胞质和细胞核中均有分布。结果表明,本研究成功获得了可溶性、具有良好免疫活性的弓形虫重组蛋白ENO2,为深入研究其生物学功能奠定了基础。     

急性乙醇中毒和外伤在蛛网膜下腔出血致死机制中的作用

目的研究人脑在急性乙醇中毒状态下tPA、MMP-2、MMP-9及AEG-1表达水平与乙醇浓度的关系,分析乙醇与外伤在蛛网膜下腔出血死亡机制中的作用。方法选取15例实际案例,提取脑组织进行组织病理学检查、心血进行乙醇浓度检测,用免疫组织化学法观察人脑干、大脑、小脑tPA、MMP-2、MMP-9及AEG-1表达水平。结果急性乙醇中毒外伤组和急性乙醇中毒组的脑组织肿胀。除小脑tPA外,tPA、MMP-2、MMP-9及AEG-1表达水平在饮酒者较未饮酒者在脑干、大脑、小脑均有升高(P0.05),MMP-2、MMP-9和AEG-1表达水平与心血乙醇浓度存在正相关(P0.05,r0.6)。结论急性乙醇中毒状态下人脑tPA、MMP-2、MMP-9、AEG-1表达较未饮酒者均显著增强,MMP-2、MMP-9及AEG-1表达水平与乙醇浓度存在相关性,乙醇对脑细胞有毒性作用。     

论我国《物权法》上的商事留置权

我国《物权法》第231条规定了商事留置权制度,其成立要件在权利义务主体、债权的发生和动产的占有等方面与民事留置权相比有所不同。商事留置权可以适用善意取得制度。在去法典化的时代,我国采用民商合一的立法例有其合理性,商事留置权制度较好地协调了民商合一的立法例与商法的特殊需求,其立法理念和立法技术值得发扬。     

Corruption Experience and Public Perceptions of Anti-corruption Crackdowns: Experimental Evidence from China

Journal of Chinese Political Science - Are citizens’ attitudes towards government’s effort to fight corruption primarily shaped by social information (such as public media and political...     

Fiscal slack or environmental pressures: which matters more for technological innovation assimilation? A configurational approach

Abstract

Prior studies suggest that fiscal slack may positively affect technological innovation in the public sector but yield inconsistency between theoretical expectations and empirical findings. To address this research gap, this study develops a configurational explanation combining fiscal slack and environmental pressures by employing fs-QCA in the context of Chinese provincial governments’ online public service provision. The findings reveal that the role of fiscal slack is environmentally dependent on explaining technological innovation and that environmental pressures seem to be more important. That is, fiscal slack is likely irrelevant to a high degree of technological innovation assimilation, while the demanding environments of multiple environmental pressures matter. Relative fiscal scarcity may be somewhat relevant to a low degree of technological innovation assimilation only when combined with a more relaxed environment. Several theoretical and practical implications are discussed.     

大数据环境下数字图书馆的基本职能

数字图书馆作为一种信息环境,其基本属性仍是收藏属性,基本职能仍是文献收藏。在大数据环境下,尤其需要重视对数字信息资源的整理与收藏,在这个过程中需要政府的强力支持,数字资源呈缴制度的建立与法律保障,数字图书馆联盟的组织,国家图书馆的核心领导,信息生产商、出版商、网络用户、技术商的合作,图书馆、档案馆、个人用户的协调,以发挥数字图书馆在大数据环境下保存和传承人类文化的基本职能。     

收入、消费与青年农民工城市融入——基于河南省的调查分析

基于河南省十八地市青年农民工调查样本,分析青年农民工的收入、消费及其对农民工城市融入的作用与影响。2013年调查样本农民工月均收入低于2011年河南省城镇非私营单位在岗职工平均月工资,青年农民工个人外显性消费呈现炫耀性特征,而集体消费则处于被抑制状态,收入水平低、消费支出短期化倾向导致农民工阶层固化,不利于青年农民工的城市融入。提高收入水平、引导农民工理性消费,是促进农民工城市融入的有效途径。     

ICF脑卒中核心分类组合功能的图形建模研究

目的依据《国际功能、残疾和健康分类(ICF)》脑卒中核心组合,探讨脑卒中相关功能类目之间的关系,绘制ICF脑卒中核心分类组合的功能地形图,为脑卒中的伤残评定提供科学理论依据。方法收集106例脑卒中被鉴定人样本,采用"最小的绝对缩减和变量选择算子",研究脑卒中ICF核心组合功能的59项类目变量之间的条件依存关系,并用图形建模分析类目之间的内在联系,绘制功能地形图。结果 59项类目之间的相互联系构成了功能地形图,b340、b735、b175和b152因关联性较高而居于地形图的中心位置。结论脑卒中功能地形图可以揭示ICF功能类目之间复杂的关系结构及关联程度。可以为应用ICF进行脑卒中的伤残评定提供科学理论依据。