The alleles present at the HLA DQ alpha locus may be typed by either allele specific oligonucleotide (ASO) probing or by restriction mapping, referred to here as amplification restriction fragment polymorphism (ARFP) analysis. ASO typing relies upon hybridization principles, whereas ARFP typing relies upon restriction site analysis. Dot-blot ASO typings which are of doubtful interpretation may be directly checked by ARFP analysis. Aliquots of the PCR products amplified using the commercial Amplitype HLA DQ alpha system are digested with suitable restriction endonucleases. Electrophoresis, blotting and detection of biotin-labelled restriction fragments provides a sensitive and robust typing method suited to forensic analysis. 相似文献
The main purpose of legal interpretation is to create conditions for the effective functioning of law and its components by clarifying their true content, which eliminates any doubts and ambiguities. The purpose of this article is: first, to analyze the provisions of current Ukrainian legislation for identifying the general approaches embodied in it and the principles for the implementation of legal interpretation activities by state power bodies; secondly: presentation on the basis of modern achievements and developments of legal science of the system of measures for standardization of such activity, bringing it in line with the needs of law enforcement practice. The solution of the set tasks was carried out using the method of philosophical dialectics, a system of general scientific and special scientific methods of cognition, which are based on the principles of objectivity, comprehensiveness, complexity. Based on the analysis of the normative material, it is concluded that there is no single harmonized approach in the Ukrainian legislation on the procedure for providing clarifications of the content of normative-legal acts. The authors give arguments on the need to clarify and streamline the legal interpretation activity by state bodies and propose a number of measures to achieve this goal. Based on the conducted analysis of the normative material, the authors outline the main problematic issues of the legislative definition of the basics of legal interpretation and provide specific proposals for improving the current legislation in this area.
Crime, Law and Social Change - Studies of bribery have been heavily influenced by the cost/benefit calculation, leaving social relationship of the involved parties under-explored. We propose the... 相似文献
Journal of Indian Philosophy - The Yogār?ava (‘the ocean of yoga’) is a Sanskrit compendium on yoga that has not been published, translated or even mentioned in secondary... 相似文献
Right-wing populist (RWP) movements have been on the rise in Western democracies. Outside of party politics, such movements regularly organize demonstrations against political elites and minority groups. At the same time, civil society coalitions have mobilized against these movements. Yet we know little about the effect of counter-demonstrations on RWP protest activities. We derive competing theoretical expectations from previous work. On the one hand, counter-mobilization reduces mobilization because the original movement is less likely to achieve its goals (expected utility/costs). On the other hand, clashes and standoffs between opposing movements facilitate mobilization through polarization and anger (identity/emotions). We empirically analyze movement–countermovement dynamics using a new city-level event dataset on street protests by the German Pegida movement and its opponents. In our quantitative analysis, we investigate how counter-mobilization is associated with the onset of Pegida protests, their intensity in terms of participant numbers, and their demobilization. Counter-mobilization does not prevent protest onset, but large counter-demonstrations are associated with larger subsequent Pegida protests, and violence against Pegida supporters reduces the likelihood that they will stop protesting. 相似文献
A novel method for the forensic analysis of commercial petroleum products is presented. In this approach, the petroleum sample is extracted with nitromethane and then separated by capillary liquid chromatography with laser-induced fluorescence detection. The addition of selective fluorescence quenching agents allows the sample to be profiled by the distribution of alternant and nonalternant polycyclic aromatic hydrocarbons (PAHs). In preliminary studies, the quenching behavior of nitromethane and diisopropylamine was established by using a standard mixture of sixteen PAHs ranging in size from two to six aromatic rings. Subsequent examination of new and used motor oil demonstrated that characteristic differences arise in the PAH content, which may allow for the unique identification of oil from a particular engine or vehicle. In addition, three brands of petrolatum jelly were successfully distinguished. Although a number of alternant alkylated and heterocyclic PAHs were found in all petrolatum samples, there were significant differences in the relative concentrations of alternant as well as nonalternant PAHs. This allowed for clear differentiation of the samples through qualitative inspection of their chromatograms as well as quantitative statistical correlation techniques. 相似文献
Public budgeting and financial management (PBFM) education has been undergoing intense scrutiny which can be seen through many professional conferences, journal symposia, and recently, the creation in 1984 of a Task Force on Budgeting and Financial Management Curriculum Reform by the Section on Budgeting and Financial Management of the American Society for Public Administration. This study analyzes pedagogic deficits in PBFM, suggests a comprehensive scope of PBFM, points to some dilemmas in PBFM curriculum design or reform, proposes some models for a curriculum reform, and raises some implementation constraints in each model. 相似文献