网状内皮组织增生病病毒HLJR0901株全基因组的克隆及序列分析

以网状内皮组织增生病病毒(REV)东北分离株HLJR0901基因组为模板,分别用6对引物扩增其全基因组,最终通过拼接和比对确定HLJR0901株的全基因组长8284bp,其基因组结构为LTR-PBS-gag-pol-env-PPT-LTR,GenBank登录号为GQ415646。运用软件将其序列与已知的其他REV全基因组进行比较。结果显示,HLJR0901株不仅与中国台湾和美国的REV毒株有较高的同源性,而且与鹅、野鸟及插入到鸡痘病毒中的REV前病毒有较近的亲缘关系,而与我国南方毒株HA9901的亲缘关系较远,表明我国流行的REV存在多样性。     

从区域治理的差异性看我国政府回应性的构建——基于我国东中西部的实证比较

政府的回应性关乎政府的公共形象,关乎社会和谐.本文从区域治理的视角,通过对我国东中西部地方政府行为的实证调查研究,利用SPSS软件计算出东中西部的平均分、标准差、差异系数和综合评定值,由此探讨我国政府在公共服务中应对公众诉求中存在的问题,并提出从善治的视角提高政府回应能力的政策建议.     

Comparison of two methods for isolating DNA from human skeletal remains for STR analysis

Abstract: The quality and efficiency of a standard organic DNA isolation method and a silica‐based method using the QIAGEN Blood Maxi Kit were compared to obtain human DNA and short tandem repeats (STRs) profiles from 39 exhumed bone samples for paternity testing. DNA samples were quantified by real‐time PCR, and STR profiles were obtained using the AmpFlSTR^® Identifiler^® PCR amplification kit. Overall, the silica‐based method recovered less DNA ranging from 0 to 147.7 ng/g (average 7.57 ng/g, median = 1.3 ng/g) than did the organic method ranging from 0 to 605 ng/g (average 44.27 ng/g, median = 5.8 ng/g). Complete profiles (16/16 loci tested) were obtained from 37/39 samples (95%) using the organic method and from 9/39 samples (23%) with the silica‐based method. Compared with a standard organic DNA isolation method, our results indicate that the published silica‐based method does not improve neither the quality nor the quantity of DNA for STR profiling.     

GC/MS衍生化测定精浆中游离支链氨基酸和果糖

目的 建立人精浆中支链氨基酸和果糖的气相质谱衍生化分析方法.方法 精液样本液化离心后,按精子活率的临床标准分为正常组和非正常组,经TMS微波衍生化,GC/MS分析两组支链氨基酸、果糖衍生物含量差异.结果 正常组与非正常组的缬氨酸,亮氨酸,异亮氨酸在相对含量上均有一定差异.正常组3种氨基酸相对含量高于非正常组.果糖分析观察到同样的特征,正常组相对含量高于非正常组.结论 精子活率低的精浆样本仍可检出支链氨基酸和果糖,其差异有助于无精子、少精子精斑的法医学鉴别.     

现代诉讼的法理性透视

现代社会的诉讼制度设计根据社会冲突的性质可主要划分为三种类型。即民事诉讼制度、刑事诉讼制度和行政诉讼制度。这三大诉讼在程序设计上有着共通的法理 ,即公正与衡平。文章从程序结构法理以及程序运作法理这两个方面对此进行了论述。随后 ,本文通过对诉讼中引入“合意机制”的思考 ,对现代诉讼的价值导向进行了反思 ,指出弘扬个体自治的深远意义。     

法理学视野中的法官自由裁量权

法官自由裁量权的定位是司法改革中的重要问题。作者从法理学的角度对法官的自由裁量权进行了较全面的探讨。文章首先分析了法官自由裁量权的内涵 ,基于此 ,作者接着从哲学、法学和实践的角度对法官自由裁量权进行了考察 ,并对自由裁量权的存在根基进行了分析 ,最后就如何缓解法官自由裁量权中个性化的涉入提出了建设性的意见。     

行政刑法视角下行政犯探究——从长春市一则案例引出

行政犯罪作为犯罪的一种,同时也是一种行政违法行为,具有双重违法性。为有效地惩处行政犯罪,其惩罚机制也有其特殊性。我国目前针对行政犯采用的是一种依附性的立法模式,就是有关于行政犯的基本罪状和法定刑都是在刑法典或刑法单行法规中规定的,只是在处罚罚则中对追究刑事责任做出笼统的宣告式表述,这种模式下激发成文法体制下刑法条文的机械化与行政法规灵活性之间的矛盾。针对我国的实际情况,笔者提出独立性的散在型立法方式,即坚持刑法典总则的原则性指导,在行政法律中设置具有独立罪名和法定刑的法律规范。这样有关行政犯的规定就可以不依附于任何其他的法律,直接依据该法规认定行政犯具体罪行的内容,有利于保持刑法相对稳定性的同时兼顾社会生活的发展与变化。     

The Crime Kuznets Curve

Objectives

Criminologists have long studied the relationship between economic conditions and crime. Empirical evidence is inconclusive, pointing at different directions. This may reflect the conflicting theoretical predictions on the relationship between these phenomena, but also the prevailing methodological choice which focuses on linear relationships even though nonlinearities are plausible theoretically.

Methods

In this paper, we revisit the empirical relationship between economic conditions and crime by exploring potential nonlinearities. We look at flexible parametric specifications that include up to a cubic term of per capita income (or one dummy for each income quintile) and nonparametric and semi-parametric specifications (such as General Additive Models). Our results are robust to controlling for the standard socioeconomic, demographic, and policy determinants of crime, as well as to including a lagged dependent variable or state and time fixed effects.

Results

We document the existence of an inverted U-shaped relationship between crime and income within US states for the period 1970-2011. Crime increases with per capita income until it reaches a maximum, and then decreases as income keeps rising. This “Crime Kuznets Curve” (CKC) exists for property crime and for categories of violent crime that can be related to economic appropriation, like robbery, and is less robust for violent crimes not connected to economic incentives. We show that this pattern cannot be explained by correlated changes in economic inequality or by changes in law enforcement.

Conclusions

In addition to providing robust evidence of the existence of a CKC, our findings lay the groundwork for studies exploring the underlying theoretical mechanisms. These should go beyond income inequality or law enforcement, and should explain why the results hold more clearly for property than for violent crime. Our findings and subsequent research to understand the underlying drivers are relevant for policy, as they suggest that violent conflict cannot be tackled solely by the trickle-down forces of economic growth.

     

Credit,crisis and contract enforcement: evidence from the Spanish loan market

A number of theoretical and empirical studies have shown that the development of credit markets is affected by the efficacy of enforcement institutions. A less explored question in this context is how these institutions interact with turns in the economic cycle and the impact of different types of legal procedures on credit market performance. This paper fills these gaps by analyzing how differences in the availability of credit and the evolution of non-performing loans ratios may be partially explained by regional variations in the quality of loan contract enforcement during recent periods of sustained growth (2001–2007) and recession (since 2008) in the Spanish economy. This research concludes that a rise in the clearance rate of executions (i.e., when a judge enforces the repayment of a debt) increases the ratio of total credit to GDP. However, the declaratory stage of the procedure (i.e., when a debt is firstly verified by a judge) does not seem to be statistically significant. A possible explanation to this finding is that, throughout the economic cycle, a relevant proportion of the defaults that take place are strategic (i.e., defaults by a solvent debtor). Furthermore, it is observed that, in regions where declaratory procedures are more efficient, less credit is declared as non-performing. The latter effect, however, is only observed after the onset of the “Great Recession” in 2008. This may be related to the increase of non-strategic defaults during a downturn.