Mucosal Cell Isolation and Analysis from Cellular Mixtures of Three Contributors

In forensic genetic analyses, mixtures of various biological materials are common samples. Micromanipulation, which is performed based on differences in cellular morphology, is an effective method for the isolation of cells from mixtures. In this study, mucosal cell was isolated from somatic cellular mixtures (blood and saliva) based on micromanipulation and a low volume‐PCR (LV‐PCR) platform. One hundred and twenty‐six parallel LV‐PCR processes were performed using an Identifiler^® kit, with 107 reactions yielding single‐source DNA profiles. Among them, 54 full profiles (50%) and 37 partial profiles (13–15 loci) were obtained. Based on the above method, we obtained a single‐source DNA profile from a cigarette butt contaminated by two victims’ blood in a murder case. The generated genotype was used to query a DNA database, and a perfect match was found.     

Genetic Variants in KCNE1, KCNQ1, and NOS1AP in Sudden Unexplained Death During Daily Activities in Chinese Han Population

Fifty‐six sudden unexplained death (SUD) cases were collected from Chinese Han population, which occurred during daily activities and were autopsy negative in comprehensive postmortem autopsy. The coding exons of potassium channel genes KCNE1, KCNQ1, and nitric oxide synthase gene NOS1AP were sequenced. A synonymous mutation, KCNE1 F54F T>C was identified in 2 SUD cases, which was absent in the control subjects. Neither genotype nor allele frequencies of KCNE1 and KCNQ1 exhibited a significant difference between the SUD and control group. In contrast, the allele frequency (p = 2.7 × 10⁻¹⁰) and genotype frequency (p = 5.9 × 10⁻⁷) of rs3751284, and the genotype frequency (p = 2.9 × 10⁻²) of rs348624 in NOS1AP of SUD were significantly different from that of controls (p < 0.05). Our study suggested that rs3751284 and rs348624 might be susceptibility loci for SUD during daily activities. Larger sample sizes and further molecular studies are needed to confirm or exclude an effect of the NOS1AP SNPs on SUD risk.     

刑事证据体系的结构探究

刑事证据体系的结构模式是证据学研究中的难点。研究证据体系结构的意义:是构建科学、实用的证据体系的前提条件;有助于发现和解决事实不清、证据不足的问题,提高办案的质量。构建刑事证据体系的理论根据:系统论,证据学,刑法学和形式逻辑。刑事证据体系的结构模式由横向和纵向两大结构组成。横向结构包括案件事实情节、犯罪构成事实和量刑情节事实等三个必备证据子系统以及选择性证据子系统即证明排除违法性或可罚性事实的证据子系统;纵向结构由证据子系统的层次结构和个体证据的层次结构两部分组成。证据子系统的层次结构包括案件事实情节证据予系统、犯罪构成事实证据子系统、量刑情节事实证据子系统以及违法性事实或可罚性事实证据子系统的层次结构;个体证据的层次结构包括主证据和辅助证据两个层次。     

犬冠状病毒TN449株M基因的克隆与转移载体的构建

用蔗糖密度梯度离心纯化犬冠状病毒TN449株病毒液,提取总RNA并反转录,同时设计1对5′端加有BamHⅠ和HindⅢ内切酶位点的引物,对病毒cDNA进行了PCR扩增,回收PCR产物将其连接入pGEM-TEasy载体并转化大肠埃希氏菌。并对阳性重组质粒菌测序和序列分析。结果,犬冠状病毒与牛冠状病毒、猫冠状病毒、猫传染性腹膜炎病毒、人冠状病毒、鸡传染性支气管炎病毒、鼠传染性肝炎病毒、猪呼吸道冠状病毒、人重症急性呼吸道综合征病毒和猪胃肠炎病毒的同源性分别是39.39%、85.41%、83.98%、36.80%、26.64%、37.23%、93.51%、29.00%和94.81%;犬冠状病毒M蛋白有3个疏水性结构域。同时构建了pET28a-CCV-M表达质粒。     

无色孔雀石绿免疫原的合成与鉴定

用化学方法合成了硝基无色孔雀石绿(NO2-LMG),还原为氨基无色孔雀石绿(NH2-LMG),经红外光谱扫描鉴定NH2-LMG为所需化合物。氨基无色孔雀石绿经重氮法处理后与兔血清白蛋白(RSA)载体蛋白相连,通过紫外分光光谱鉴定,偶联产物与其前体物相比吸收峰发生了飘移,说明偶联成功。通过动物免疫试验和ELISA抗体特异性试验对该人工合成抗原免疫原性和与无色孔雀石绿反应原性的鉴定,表明成功合成了无色孔雀石绿免疫原。     

韩国女性参政的社会环境浅析

近年来,随着韩国经济社会的持续发展、女性教育水平的提高和职业女性的增多,韩国女性的政治参与意识不断提高,同时政府也陆续出台了一些有利于男女地位平等和女性参政的政策法规.但总体说来,由于韩国是一个崇尚儒家文化的国家,因此即使在现今社会,韩国女性的政治参与程度与其他领域相比,仍处于一个相对较低的水平,对未来的发展须持谨慎乐观的态度.     

对非法持有、私藏枪支、弹药罪的理解

我国刑法分则和最高人民法院司法解释均规定了非法持有、私藏枪支、弹药罪，对“持有”和“私藏”行为进行了界定，但其合理性值得研究。正确理解和定性“持有”和“私藏”两种行为，以期合理区别其在实践中的情形。     

从富士康事件正视目前的劳资关系和经济转型

当前,正是中国加快经济发展方式转型的关键时期,富士康跳楼事件的发生,无疑为我们深入认识中国经济转型的必要性和重要性,提供了很好的视角.我以为,至少有以下几点需要我们认真思考.     

中国建筑企业参与国际竞争的博弈分析

建筑市场的国际化和建筑企业的国际竞争已经成为中国建筑企业必须面对的问题,鉴于此,中国建筑企业掌握国际竞争中博弈原理非常重要.本文运用静态博弈与动态博弈的原理和方法,讨论了建筑企业对于在未进入的市场以及占领了的市场中如何击退竞争对手和获取最大利润的问题,最后提出了我国建筑企业参与国际竞争的若干对策.     

周恩来与抗战时期的叶挺

叶挺在其革命生涯中,多次得到用恩来的帮助与指导,尤其是在抗日战争时期,叶挺受中共中央之托组建新四军,在遇到种种困难之际,总是得到周恩来的及时引导与关怀,使得他能正确地对待与处理相关问题,为党的事业做出重要的贡献.