Isolating cells from non-sperm cellular mixtures using the PALM microlaser micro dissection system

Use of the Positioning Ablation Laser MicroBeam (PALM) microlaser system to isolate specific cellular components from somatic cellular mixtures (blood and saliva) prior to DNA extraction and typing is compared with routine DNA extraction and typing of the same mixture samples. Mixtures of blood and saliva at differing ratios generated complex DNA profiles that included allele peaks originating from each of the donors, or just those of the major contributor. Isolation of cells with the PALM microlaser prior to DNA extraction allowed informative, single-source, DNA profiles to be generated from a known cell type/origin.     

The transfer of human DNA by Lucilia cuprina (Meigen) (Diptera: Calliphoridae)

Blowflies leave deposits, termed artefacts, through the processes of excretion and regurgitation. To date, little consideration has been given to the possibility of adult blowflies consuming biological material and subsequently acting as vectors of human DNA through these artefacts. In this study, Lucilia cuprina (Meigen) (Diptera: Calliphoridae) were fed either human blood or human semen ad libitum and their artefacts were analysed for human DNA content. Samples containing 1, 10, 30 and 50 artefacts were tested. Quantifiable and typeable levels of human DNA were found in samples derived from both food sources, and even in samples containing a single artefact. Semen-derived artefacts contained significantly more human DNA than artefacts produced after a blood meal. Consequently a smaller number of artefacts was required to collect sufficient DNA for genotyping. These findings are forensically important as it provides investigators with another potential source of DNA at a crime scene where a body has been moved, or an attempt has been made to clean up biological material. They also highlight how fly artefacts could potentially contaminate and compromise evidence.     

The difficult issue of age assessment on pedo-pornographic material

The issue of juvenile pornography has seen an increase in the past few years of the number of expert opinions requested to forensic pathologists, paediatricians and other various experts within the forensic and medical fields concerning the age of represented individuals. Regardless of the entity of the problem, no actual method exists which can allow us to give an objective and scientific answer, particularly in the postpubertal stage. Using parameters related to sexual maturation can be very dangerous. Nonetheless some experts still insist with similar types of “expertises”. This study aims at verifying the ability of different experts in assessing age of postpubertal individuals represented in pornographic material. Results underline the difficulties and major uncertainties of age evaluation by visual observation of photographic material particularly when the subjects have reached the sexual maturation stage – and therefore in verifying whether the individual is above or below 18 years of age (an important age limit for most European countries as far as this type of crime is concerned). Furthermore the study stresses the need both to search for an alternate approach and to apply extreme caution in judicial evaluation.     

Decentralization as a conflict transformation tool: The challenge in Kosovo

Perspectives are divided on whether decentralization can ease ethnic conflict. This article considers whether asymmetric decentralization reforms in Kosovo have reduced tensions between Kosovo Albanians (K‐Albanians) and Serbs (K‐Serbs). We argue that because decentralization has been linked to Kosovo's sovereignty in the years after the NATO bombings, during the final status talks, and after independence, it has not achieved intended outcomes throughout the territory of Kosovo. Instead of assuaging tensions and generating allegiance to the central government, decentralization has re‐inforced ethnic divisions and strengthened K‐Serb ties to Serbia, particularly in northern Kosovo. Concessions to majority Serb municipalities in Kosovo have been seen by K‐Serbs as a bribe to buy acceptance of independence, while K‐Albanians question their leaders' continued policy of asymmetric decentralization. Since independence, there have been some encouraging developments in southern municipalities, where K‐Serbs have participated in municipal elections organized by Pristina. Based on Kosovo's experience, we argue that policy‐makers must consider the impact of decentralization reforms at multiple levels of conflict. Further, although we find that decentralization may engage minorities in political processes if reforms are attempted after the establishment of a central government, we caution that it must be combined with policies to encourage interaction and dialogue between ethnic groups if it is to assuage conflict. Copyright © 2010 John Wiley & Sons, Ltd.     

Wachsende Lohnungleichheit in Deutschland

Based on the thesis of growing overall inequality in the German society, this paper discusses changes in the inequality of wages for the period between 1998 and 2006. Our objective is to identify differences in real wage growth for various occupational classes. The theoretical foundation of the article is based on economic as well as sociological concepts. With regard to recent findings of labor economists, one could postulate that market forces driven by technological changes and the corresponding growth in the relative demand for highly-skilled labor have led to an increase in wage inequality (skill-biased technological change, SBTC). In contrast, a structural theory approach, based on the work of Aage B. Sørensen, would argue that increasing wage inequality is due to changes in the extent to which certain labor market groups are able to generate rents. For the empirical analysis, data from the German Socio-Economic Panel (GSOEP) and data from the BIBB/IAB-Survey are used. Our results show that people in lower occupational classes belong to the losers of the general income dynamics in Germany between 1998 and 2006. Moreover, the inclusion of a broad variety of characteristics of the occupational position reveals that the growth of wage inequality in Germany can be explained by skill-biased technological changes, but also by changes in rent generating processes.     

Zwischen pragmatischem Verstehen und theoretischen Perspektiven. Politikwissenschaftliche Forschung zur Bundesrepublik Deutschland

Since 1949 we have witnessed paradigmatic changes in political science research concerning the field of German politics. The focus of this article is on the most important political institutions in Germany: Federal President, Federal Government, Federal Parliament, Bundesrat (quasi second chamber)/federalism and Federal Constitutional Court. German political science has produced an impressive number of publications on the German political system with a wide range of approaches and of high quality. What is to some extent lacking, however, is an innovative combination of the improved knowledge created with regard to the functioning of institutions in Germany on the one hand and of middle-range theories of political science on the other, especially of theories developed in international and comparative discourses.     

Social class,socialization practices,and psychosomatic ailments in adolescents

The relationships among social class, socialization practices, and psychosomatic ailments have been reported by several psychological and sociological studies. However, a careful review of these studies reveals some contradictory results. In addition to inadequate sample size, these studies have been carried out on children of preschool and grade school ages. We collected data from 1518 high school aged adolescents who had unbroken families and who might be regarded as products of their families' class and socialization practices. Our findings suggest definite effects of socioeconomic status on the mental health of the developing female children. The effects of some socialization practices, such as parental dominance, restrictiveness, punishment types, and warmth, on psychosomatic incidences are further examined. Implications of the present study are discussed.Received his Ph.D. from Vanderbilt University. Major interests include sociology of mental illness and deviant behavior. Currently he is working on a book concerning the mentally ill.Received his Ph.D. from Iowa State University. Major interests include methodology and social disorganization. Current research is in social gerontology and applied statistics.     

'Give it the Shade': Paul Celan and the Politics of Apolitical Poetry

This article analyses the political impact of Paul Celan's poetry to illustrate the potential for social transformation contained in seemingly apolitical practices of speaking and writing. Celan's work epitomizes the search for thinking space in the immediate post-war period. Much of the German language at the time was so closely associated with the horrors of the Nazi regime that it was difficult to address critically many important social issues. In dealing with this dilemma, Celan recognized that politics ought to deal with language, with how we have come to objectivize the dilemmas that surround us. His poetry tried to stretch the boundaries of the German language such that it became possible to speak once more, critically, dialogically, humanely. By scrutinizing the potential and limits of this process, the present article addresses various issues that are at stake in the interaction between language, politics and society.     

Electoral terms and terrorism

Many terror attacks occur at the beginning of electoral terms. We present a game theoretical model with incomplete information to account for this empirical pattern. Both terrorists and governments can be of weak or strong types. We find that the risk of terror attacks is highest at the beginning of electoral terms, because striking early allows the terrorists to collect valuable information about the government’s type, and also because terrorists know that even initially weak governments sometimes retaliate to show toughness closer to an upcoming election. The model’s predictions are consistent with anecdotal evidence.