24个基因座复合扩增系统的应用

目的建立24个基因座的复合扩增系统,并对其性能指标进行评价。方法选择24个基因座（包含D8S1179、D5S818、D2S1338、D18S51、D6S1043、D2S441、D3S1358、vWA、D19S433、D16S539、CSF1PO、Penta D、D22S1045、D13S317、D1S1656、D7S820、TPOX、Penta E、D10S1248、TH01、D12S391、D21S11、FGA等23个STR基因座及1个Amelogenin基因座）;合成引物,上游端标记荧光染料;收集DNA数据库建库血痕及口腔拭子样本及相关11种动物样本,采用本文方法进行复合扩增;并对方法的准确性、灵敏度、稳定性、种属特异性、检材适用性、混合样本的检验等系统性能指标进行验证。结果本文复合扩增系统对最长保存9年的各种血痕检材完整分型成功率为100%,且均衡性良好,无非特异性扩增,口腔拭子的成功率为97.8%,灵敏度达125pg,对含有抑制剂样本,在血红素浓度≤600μmol/L,腐殖酸浓度≤50ng/μL时检出效果稳定,种属特异性好。结论本文24个基因座复合扩增系统在DNA数据库建设中具有较好的应用价值。     

“海上丝绸之路”的新内涵及海南省的作用

中国国家主席习近平访问东盟国家时提出共同建设21世纪海上丝绸之路,新的时代赋予了海上丝绸之路新的内涵和意义。本文提出推动海上丝绸之路建设的举措,探讨海南省在建设海上丝绸之路中的作用。     

部门规章为什么没有行政许可设定权--部门规章功能分析

我国<行政许可法>为什么没有授予部门规章行政许可设定权,其根本原因就在于我国<立法法>所规定的部门规章属于一种执行性行政立法,其与地方政府规章既有执行性,又具有一定范围内的创设性的立法职能不同.<行政许可法>的这一安排,体现了基本立法制度对行政立法制度的决定性作用,同时也反映了我国立法理念的进步.     

Understanding the Evolution of Rice Technology in China – From Traditional Agriculture to GM Rice Today

This paper provides an historical survey of the evolution of rice technology in China, from the traditional farming system to genetically modified rice today. Using sociotechnological analytical framework, it analyses rice technology as a socio-technical ensemble – a complex interaction of material and social elements, and discusses the specificity of technology development and its socio-technical outcomes. It points to two imperatives in rice variety development: wholesale transporting agricultural technology and social mechanism to developing countries are likely lead to negative consequences; indigenous innovation including deploying GM technology for seed varietal development and capturing/cultivating local knowledge will provide better solutions.     

吸毒者头发中海洛因、6-单乙酰吗啡、吗啡、可待因和乙酰可待因的分析及评价

目的确定海洛因吸毒者头发中海洛因、6-单乙酰吗啡（6-MAM）、吗啡（MOR）、可待因（COD）和乙酰可待因（AC）的浓度并考察其与国际毛发分析协会（Society of Hair Testing,SOHT）建议标准的适用性。方法 50个头发样品经冷冻研磨处理后采用液相色谱-串联质谱方法分析。结果所有样品中均检出6-MAM,并且浓度高于SOHT所建议的0.2 ng/mg,其中海洛因、6-MAM、MOR、COD和AC的浓度与其它研究报道无明显差异。头发中6-MAM：MOR的比率在0.15~36.27范围。结论由于各实验室间毛发样品前处理方法不同,而且存在吸食毒品中成分、剂量、吸食方式、代谢、头发颜色等诸多个体差异,本研究认为采用头发中6-MAM：MOR的比率大于1.3标准难以应用于鉴定海洛因吸毒。     

关于推进资源型城市可持续发展的建议

该文指出,中国一些资源型城市目前面临产业结构不合理、生态衰退和环境破坏严重、可持续发展发展问题突出等严峻形势.为此,该文结合国内与国外转型发展较好的城市案例,建议中国资源枯竭型城市转型应该依靠科技创新,因时、因地制宜,不同发展周期、不同资源类型的城市转型应选择不同转型路径,同时注意发挥企业在资源型城市发展中的主体作用,并把保障和改善民生作为资源型城市转型的出发点和落脚点.     

培养造就一支宏大的社会工作人才队伍

建设和培养造就一支社会工作人才队伍是我国建设服务型政府的重中之重。为此,应该加快建立和完善社会工作人才的资格认证、注册管理、从业规范等各项制度,系统构建社会工作人才的教育及培训体系,加大社会工作人才队伍的投入保障,解决社会工作人才发展的平台。本文在论述以上问题的同时,借鉴了发达国家社会工作人才队伍建设经验,对社会工作的概念、社会工作者的要求、社会工作的视角、社会工作者专业素质及其培养、社会工作者评估及监察制度等问题进行了初步探讨。     

非政府组织在应急管理中的作用

在应急管理中,如何充分发挥非政府组织的作用已受到现代各国的广泛重视。文章根据国内外应急管理的实践,梳理了非政府组织在应急管理中的角色和作用,归纳总结了国外鼓励支持非政府组织参与应急管理的流行做法和经验,探讨了提高我国非政府组织参与应急管理能力的思路选择。     

Re-branding without re-developing: constraints of Hong Kong's ‘Asia's World City’ brand (1997–2007)

Abstract

Interest among academics towards Hong Kong's global status has gradually waned since 1997. However, identifying Hong Kong's position on the international platform is essential if the Special Administrative Region's competitiveness is to be distinguished from that of regional rivals. The positioning challenge not only results from the sovereignty retrocession, but also from the rapid pace of globalization and the intensified competition from major cities in the Greater China Region. Instead of focusing on the much-researched topic of the direct competition between Hong Kong and Shanghai or Singapore, an exploration of Hong Kong's hitherto little-researched re-branding experience not only throws light on Hong Kong Special Administrative Region's policy orientation, but is also valuable in understanding how other cities in the region conducted similar exercises. This article reviews the overall evolution of the ‘Asia's World City’ (AWC) discourse as promoted by the Hong Kong Special Administrative Region from 1997 to 2007. It starts by reviewing the international identity of post-colonial Hong Kong as background to the discourse, in order to reconstruct various theoretical assumptions that led to the re-branding exercise. The second section examines the purpose and evolution of the AWC campaign, and identifies the problems within the discourse. The concluding section focuses on the unrealized theoretical assumptions and other administrative and ideological constraints that the HKSARG faced in the exercise as a means to explaining the overall limited achievements of the campaign and proposes possible ways to remedy the shortcomings.     

Residential location,transportation, and welfare‐to‐work in the United States: A case study of Milwaukee

Abstract

This article addresses two questions about spatial barriers to welfare‐to‐work transition in the United States. First, what residential and transportation adjustments do welfare recipients tend to make as they try to become economically self‐sufficient? Second, do these adjustments actually increase the probability that they will become employed?

Analysis of 1997–2000 panel data on housing location and automobile ownership for Milwaukee welfare recipients reveals two tendencies: (1) to relocate to neighborhoods with less poverty and more racial integration and (2) to obtain a car. Results from binary logit models indicate that residential relocation and car ownership both increase the likelihood that welfare recipients will become employed. These findings suggest that policies should aim to facilitate residential mobility for low‐income families and improve their neighborhoods, rather than simply move them closer to job opportunities. The findings also suggest a critical role for transportation policy in reducing unemployment.