马病毒性动脉炎和马鼻肺炎抗体时间分辨荧光免疫分析检测方法的建立

以基因重组抗原包被96孔板,Eu3+标记兔抗马IgG,建立了马病毒性动脉炎和鼻肺炎抗体的时间分辨荧光免疫分析检测方法,对其特异性、灵敏性、稳定性进行了分析,并与进口ELISA试剂盒和微量血清中和试验进行了比较。结果显示,该方法交叉反应不明显,特异性好,灵敏度高于ELISA方法;该方法的批内和批间变异系数分别为2.68%～4.43%和4.21%～7.25%,Eu3+标记物可稳定保存5个月以上。证实,建立的马病毒性动脉炎和马鼻肺炎抗体的时间分辨荧光免疫分析检测技术灵敏度高,特异性和稳定性好,具有很好的应用前景。     

INTERNET INTERMEDIARIES’ LIABILITY FOR ONLINE ILLEGAL HATE SPEECH

Considering the prevalence of online hate speech and its harm and risks to the targeted people, democratic discourse and public security, it is necessary to combat online hate speech. For this purpose, internet intermediaries play a crucial role as new governors of online speech. However, there is no universal definition of hate speech. Rules concerning this vary in different countries depending on their social, ethical, legal and religious backgrounds. The answer to the question of who can be liable for online hate speech also varies in different countries depending on the social, cultural, history, legal and political backgrounds. The First Amendment, cyberliberalism and the priority of promoting the emerging internet industry lead to the U.S. model, which offers intermediaries wide exemptions from liability for third-party illegal content. Conversely, the Chinese model of cyberpaternalism prefers to control online content on ideological, political and national security grounds through indirect methods, whereas the European Union (EU) and most European countries, including Germany, choose the middle ground to achieve balance between restricting online illegal hate speech and the freedom of speech as well as internet innovation. It is worth noting that there is a heated discussion on whether intermediary liability exemptions are still suitable for the world today, and there is a tendency in the EU to expand intermediary liability by imposing obligation on online platforms to tackle illegal hate speech. However, these reforms are again criticized as they could lead to erosion of the EU legal framework as well as privatization of law enforcement through algorithmic tools. Those critical issues relate to the central questions of whether intermediaries should be liable for user-generated illegal hate speech at all and, if so, how should they fulfill these liabilities? Based on the analysis of the different basic standpoints of cyberliberalists and cyberpaternalists on the internet regulation as well as the arguments of proponents and opponents of the intermediary liability exemptions, especially the debates over factual impracticality and legal restraints, impact on internet innovation and the chilling effect on freedom of speech in the case that intermediaries bear liabilities for illegal third-party content, the paper argues that the arguments for intermediary liability exemptions are not any more tenable or plausible in the web 3.0 era. The outdated intermediary immunity doctrine needs to be reformed and amended. Furthermore, intermediaries are becoming the new governors of online speech and platforms now have the power to curtail online hate speech. Thus, the attention should turn to the appropriate design of legal responsibilities of intermediaries. The possible suggestions could be the following three points: Imposing liability on intermediaries for illegal hate speech requires national law and international human rights norms as the outer boundary; openness, transparency and accountability as internal constraints; balance of multi-interests and involvement of multi-stakeholders in internet governance regime.     

健脾通里中药芪黄煎剂对胃切除大鼠肠黏膜生物屏障损伤的影响

目的 观察健脾通里中药芪黄煎剂对大鼠胃切除手术创伤后肠黏膜生物屏障损伤的保护作用及其机制.方法 将90只SD大鼠随机分成空白对照组,假手术组,肠内营养组,芪黄煎剂低、中、高剂量组,每组15只.采用胃切除术复制大鼠肠黏膜损伤模型.空白对照组和假手术组术后自由饮水进食,不给予肠内营养液和中药;肠内营养组术后注射肠内营养液;...     

红外光谱结合化学计量学快速鉴别不同环境发汗丹参

目的 采用红外光谱法测定空旷阴凉、空旷光照及室内环境下堆积发汗丹参中水提物及醇提物特征,快速鉴别其质量.方法 采用傅里叶红外光谱技术结合化学计量学建立主成分分析-马氏距离(principal component analysis-Mahalanobis distance,PCA-MD)判别模型鉴别分析不同环境下发汗丹参...     

海滨大戟化学成分研究

目的 研究海滨大戟Euphorbia atoto Forst.地上部分的化学成分组成.方法 采用硅胶柱色谱、Rp-18反相柱色谱、Sephadex LH-20凝胶柱色谱以及重结晶方法进行分离纯化,并根据理化性质及核磁共振波谱和质谱数据鉴定化合物结构.结果 从海滨大戟地上部分95％乙醇提取物中分离得到14个化合物,分别鉴定为mayolene-16(1)、(+)-(R)-脱-O-甲基拉西奥地丁(2)、熊果烷(3)、α-香树脂醇(4)、伪蒲公英甾醇(5)、(3β,24R)-大戟烷-7,25-二烯-3,24-二醇(6)、29-nor-21-α-H-hopane-3,22-dione(7)、Hollongdione(8)、5α-豆甾醇-3,6-二酮(9)、22E,24R-麦角甾烷-7,22-二烯-3β,5α,6β-三醇(10)、菜油甾醇(11)、豆甾醇(12)、β-谷甾醇(13)、芹菜素(14).结论 首次从海滨大戟中分离得到14个单体化合物,其中化合物mayolene-16(1)为新的天然产物.     

古罗马社会的女性、身体与政治——以莎士比亚笔下的伏伦妮娅形象为中心

普鲁塔克的《希腊罗马名人传》对莎士比亚罗马历史剧《科里奥兰纳斯》有深刻影响。不过,普鲁塔克笔下以母亲和罗马好公民双重身份三次出现在“家”和“敌营”两个空间的伏伦妮娅形象略显单薄,止于一种笼统的静态之美,而莎士比亚笔下以女人、母亲、国家政治的隐性操手三重身份六次显性在场、三次隐性在场的伏伦妮娅,展现出多维空间下的悖论之美,表现出一种理性、欲望、激情与活力交织下的女性动态之变与灵魂之美。莎士比亚从生活真实出发,在广阔的历史背景和富有时代特征的典型环境下通过对伏伦妮娅形象的重塑,反映女性在家庭领域和政治领域的生存现状。他围绕女性—身体—政治这一脉络,通过对伏伦妮娅的隐性政治参与与其家庭教育方式之间关系的想象与构建,将家庭伦理与国家政治有机联系在一起,以彰显其家国同构的思想。     

马克思主义中国化缘起的逻辑理路与历史经验——学习贯彻党的十九届六中全会精神

马克思主义中国化是将马克思主义基本原理与中国具体实际相结合,不断形成具有中国特色的马克思主义理论成果的过程。马克思主义本身所具有的理论完备性与实践创新性、科学真理性与革命阶级性、世界普遍性与民族特殊性等内在发展特质构成了马克思主义中国化缘起的理论逻辑。近代中国的救亡图存之路、各种思潮的争论与抉择、马克思主义在中国的传播以及中国共产党的成立构成了马克思主义中国化缘起的现实逻辑。马克思主义中国化由可能转变为现实,最根本的原因在于早期中国共产党人充分做到了理论联系实际,在科学对待马克思主义的基础上,对中国国情进行深入剖析,以实现民族独立、人民解放、国家富强、人民幸福为根本任务,带领人民进行革命实践,使马克思主义中国化逐渐拉开历史帷幕。     

全面构建虚拟世界中的和谐社会——我国网络监察工作现状及发展探析

随着互联网技术的不断发展,人们依赖互联网的程度不断加深,互联网安全也越来越受到人们的重视。现阶段,网络犯罪案件调查和网络监察工作由公安机关公共信息和网络监察部门负责,但由于我国网络监察工作起步较晚、立法不健全等原因,造成了许多网络违法犯罪案件无法立案或立案后打击不力的问题。笔者从我国互联网发展现状及网络违法犯罪的形式入手,阐述了网络监察工作的任务,揭示出现阶段网络监察工作存在的问题,探讨了网监工作的发展趋势。     

民族伦理翻耕与重构之筹划

民族伦理所呈现的地方性知识谱系是一个蕴涵多元参照点的开放结构,它为伦理精神的翻耕与重建予以资源储备上的贡献。翻耕是对民族伦理资源的深挖与对百家伦理见解的吞吐;而重建是基于对民族伦理病灶的镜见与对民族伦理的现代病根的祛除。研究认定民族伦理的内核依然可以新旧相续,对民族伦理的翻耕与重建的筹划需借力对民族伦理的全新解释,并以此全新解释而获致地方性道德知识谱系的维护与民族伦理创见的突破。     

矫治工作者常见心理问题及其应对

矫治工作者是监所从事矫治工作的主体,对他们在工作中经常出现的心理问题进行研究,对矫治工作者队伍建设和素养提高具有重要的意义。矫治工作者常出现的心理问题包括常见认知偏差、情绪问题以及人际关系问题,矫治工作者可以通过自我接纳、内省以及专业成长等积极途径和方法应对这些问题。