气相色谱-串联质谱法分析尿和血中除草剂百草枯

目的建立尿和血中百草枯的离子交换固相萃取-气相色谱-串联质谱分析方法。方法尿样加内标乙基百草枯,用732阳离子交换树脂提取;血样加内标乙基百草枯,用三氯乙酸凝聚蛋白质后取上清液用732阳离子交换树脂提取。提取物用硼氢化钠在水溶液中碱性条件下还原,还原物用有机溶剂提取进行气相色谱-串联质谱法分析。结果尿和血中百草枯的提取率分别为76％和74％,检测限分别为2ng／mL和10ng／mL,尿添加百草枯100ng／mL和血添加百草枯500ng／mL水平的回收率分别为99．6±5．6％和99．3±7．6％（Mean±CV）。结论本文建立的分析方法灵敏度高,能够满足中毒致死案件检验及临床毒物检验的需要。     

无效合同诉讼时效问题探析

无效合同的诉讼时效确定问题一直是司法实践中的难点.确认合同无效属法律对合同效力的价值评判,不应受诉讼时效的限制.而无效合同产生的返还财产和赔偿损失请求权应受诉讼时效的限制.无效合同产生的返还财产和赔偿损失请求权诉讼时效期间的起算,应根据当事人知悉其权利受到侵害的三种具体情形分别予以确定.     

论传媒与司法的博弈及平衡

传媒具有客观性、主动性和动态反映性特点.在联系案例的基础上研究司法与传媒的博弈关系,认为传媒与司法之间可能存在冲突.因此需要平衡,同时二者之间的共同点又是平衡的基础.我们的正确选择是如何实现二者之间的动态平衡关系.     

刑事司法环境下的“隐形程序”

"隐形程序"作为我国刑事司法内部环境的组成部分,具有不同的渊源和表现形式,且对刑事司法活动具有重要影响。本文在对我国刑事司法中"隐性程序"存在的原因及其对刑事司法的负面影响进行系统分析的基础之上,提出对其进行合理化改造建议在刑事司法过程中,应当贯彻程序公开、透明的原则,充分保障当事人的合法权益,以营造良好的刑事司法环境。     

用酶标抗人精液单克隆抗体A_(10)C_6建立ELISA斑点法快速鉴定人精(液)斑

作者用过氧化物酶标记自己研制的抗人精液单克隆抗体A10C6建立了简便快速鉴定人精斑的ELISA斑点法，直接通过酶标抗体上的酶催化底物显示颜色变化，来判断结果，阳性呈灰色斑点，阴性为无色。其结果只对人精斑浸出液显示阳性斑点，对人的其他组织器官及体液均呈阴性，对动物精斑也无交叉反应，精斑浸出液稀释到1：3200倍仍呈阳性结果。     

黑社会性质组织犯罪的经济分析

黑社会性质组织犯罪的本质特征在于以违法犯罪的手段对内进行人身控制,对外进行经济控制。要有效地惩罚和预防黑社会犯罪,必须提高黑社会性质组织犯罪的预期刑罚成本,提高由于刑罚带来的机会成本的损失,并且通过实施社会效益最大化的公共政策,争取以最小的成本,取得最大的控制犯罪的收益。     

论“圈地”现象背后地方政府的利益追求

本文从近期的“圈地”热潮开始谈起,分析了土地与土地权,国家与政府,中央政府与地方政府三组关系,讨论了其对征地的影响,并将重点落到地方政府上面。然后,用经济学的有关理论论证地方政府“圈地”追求的并不是一般意义上的垄断利润,而是其他一些利益。文章提出了建立一套利益制衡机制以遏制地方政府利益追求,试图以此来规范征地行为。     

Genetic analysis of 18 X-linked short tandem repeat markers in Korean population

The population genetic data of 18 X-chromosomal short tandem repeat (STR) markers DXS6807, DXS8378, DXS9895, DXS9902, DXS6810, DXS7132, DXS981, DXS6800, DXS9898, DXS6789, DXS101, DXS6797, GATA172D05, GATA165B12, HPRTB, GATA31E08, DXS8377, and DXS7423 were analyzed in samples of unrelated 220 males and 181 females from Korean population. The exact test for genotype distribution of the markers showed no significant deviation from the Hardy-Weinberg equilibrium. Allele frequencies between male and female samples were not significantly different in all examined markers. All examined males and females showed different hemizygotic haplotype and combined genotypes, respectively. Four cases of mutation were found in GATA172D05, GATA31E08, DXS7132, and HPRTB from the analysis of 95 father-child-mother trios. Details of X chromosomal STRs in Koreans would be useful in paternity tests and forensic purposes as well as whole X-chromosomal mapping studies.     

Study on the application of parent-of-origin specific DNA methylation markers to forensic genetics

In paternity test, especially in motherless cases, the allele inherited from father (obligatory gene, OG) often cannot be determined. The paternity exclusion probability (PE) of a genetic marker is reduced considerably. Therefore, it is necessary to develop a new technique, by which the parental origin of alleles can be determined without genealogical analysis. In this paper, we explored the possibility of using parent-of-origin specific DNA methylation markers to determine the parental origin of alleles, choosing the imprinted single nucleotide polymorphism (SNP) locus rs220028 (A/G) as a model system. We typed the SNP by mutagenically separated PCR (MS-PCR). The frequencies of alleles were A = 0.5085, G = 0.4915; the unbiased heterozygosity was 0.5020. In order to discriminate between the maternal allele and paternal allele, post-digestion MS-PCR, a novel PCR based methylation analysis and SNP typing technique was developed and performed on 18 heterozygous children, and the methylated maternal allele was detected specifically. As a pilot study on the use of epigenetic markers in forensic genetics, our results demonstrated the feasibility of using parent-of-origin specific DNA methylation markers to determine the parental origin of alleles.     

构建我国证人反对被迫自证其罪特权制度的设想

我国证人出庭作证制度极不完善,尤其是证人权利义务设置失衡,证人的应有权利缺乏保障,导致证人出庭作证难,拒证现象严重。反对被迫自证其罪特权是证人的一项必不可少的权利,它已得到世界众多国家以及联合国公约的认可。构建我国证人反对被迫自证其罪特权制度对于改善我国证人作证的现状,切实保障证人合法权益是非常重要的。