微滴式数字PCR技术用于生物样品种属鉴定和绝对定量

目的 运用微滴式数字PCR技术进行生物样品的种属鉴定和绝对定量.方法 选择人mtDNA两个编码基因ND4和16S rRNA,设计特异性引物与探针,用人来源及常见动物样本验证种属特异性,再用重组质粒和2组共16份人来源生物检材,倍比稀释.使用微滴式数字PCR技术进行种属检验和绝对定量,验证其灵敏度和稳定性.结果 人重组质粒FAM （ND4）可进行人来源样品的检测,其检测结果与各级稀释梯度基本吻合,微滴式数字PCR技术可以检测出反应体系中低至单拷贝的DNA.结论 微滴式数字PCR技术可以进行生物样品的种属鉴定和绝对定量,并具有很高的灵敏度和特异性,可应用于日常法医物证检验.     

医疗纠纷处理机制的现状研究

医疗纠纷已成为困扰和影响医院正常工作,甚至威胁医护人员安全,扰乱社会和谐的问题。如何公平、公正、合法、高效地处理医疗纠纷已成为目前不容忽视的问题。本文通过协商、行政调解,诉讼和第三方调解等几种目前医疗纠纷处理机制进行分析研究,以求为更好地处理医疗纠纷提供有益的参考。     

贵州文化浅论

文章对贵州文化的定义、外延、内涵、特征文化价值等作了简要的论述,并认为贵州文化、贵州文化资源在发展贵州就、发展贵州文化产业、文化商品方面,在社会、经济、文化三者协调发展方面,具有无比的文化价值和广阔的前景。     

Small City,Big Ambition

If China is indeed the factory of the world, then Yiwu, a small city in eastern Zhejiang Province, would be its headquarters. A small commodity trade center established in this rural area in early 1980s as a test field to spearhead local economic development has grown fast to become what is now the world's largest market for small commodities.     

The Soft Link

An NGO's efforts to promote people-to-people diplomacy between Chinaand the United States It was on Harvard's campus that Liu Xi hit upon the idea to set up a program to bring the real China to more Harvard students.Liu attended a master's program at the Harvard Kennedy School (HKS) from 2013 to2015.While studying in the U.S.,she realized that many Americans have a biased view of China.     

经济社会一体化:多目标平衡与治理机制

工业化后期经济发展可持续的源泉,在于服务业结构升级和消费结构升级所提供的效率补偿效应,高质量发展的关键是人力资本积累和要素质量升级。经济发展服务于社会发展,是工业型社会转变为知识型、福利型社会的动力,相应地围绕社会发展目标制定实施经济政策,成为新常态下效率/福利目标动态平衡的重要取向。     

边缘青年情绪心理危机的测量与疏导——基于浙江新生代农民工的调查

近年来,多起恶性事件的发生预警了边缘青年受忽视的情绪心理健康问题。作为主要的边缘青年群体,新生代农民工处于青春期这一特定的人生阶段,其心理状态、社会交往及人际交往中的情绪、情感体验都具有一定的特殊性。通过实证研究,本文调查了新生代农民工在当地人际交往中的情绪心理,研究发现,亲密的人际关系对他们的情绪心理健康最具重要性,但实际可依赖的倾诉渠道比较有限。此外,本研究还测量了交往中潜在的情绪心理危机程度,深入挖掘了造成人际交往的内隐性情绪心理问题的主要诱因,建立了有关外部行为的预测指标,探索疏导这一青年群体在人际交往中所存在的情绪问题,以帮助他们提高人际关系的品质,有效预防情绪心理危机。     

Discrimination of phenethylamine regioisomers and structural analogues by Raman spectroscopy

In this study, the Raman spectra of 21 phenethylamines were obtained using far‐red excitation (785 nm). The distinguishing ability of Raman for phenethylamines, especially for phenethylamine regioisomers and structural analogues, was investigated. Here, the evaluation of a cross section of Raman spectra demonstrated that all types of phenethylamines were distinguishable, even for certain structural analogues with high spectrum similarity. Raman exhibited high distinguishing ability for phenethylamine regioisomers that differ in the substitution position of halogen, methoxy, alkyl, or other substituted groups; as well as for structural analogues containing different groups, such as furanyl, 2,3‐dihydrofuranyl, halogen, and alkyl substituted at the same position. The Raman spectra for homologues with differences in only a methyl group were found to be highly similar; however, their spectra demonstrated small but detectable differences. Four analogue mixtures and 59 seized samples were also analyzed to study the practical use of the Raman method in forensic field. 95% of the seized samples were correctly identified, which significantly validated the ability of Raman method in identifying the correct isomers. Accordingly, this study provides a non‐destructive, high‐throughput and minimal sample preparation technique for the discrimination of phenethylamines.     

在监管中吸纳:官办社团参与城市管理的系统分析——以A市城市管理义务工作者组织为例

针对当代中国城市管理过程中出现的吸纳型监管模式,可以从政治系统论视角进行分析.从输入、输出和反馈的过程来看,吸纳型监管不同于西方自由竞争式的监管内涵,它来源于作为执行输出端的政府职能的折冲,服务于作为支持输入端的政府信任提升,体现出政府存在的需求输入及反馈社会的输出性结果.从监管型国家建设目标来看,以城管执法和官办社团开展的城市吸纳型监管新模式,不等同于社会力量参与治理,难以达到提升监管合法性和有效性的目标.其根源是在城市管理存在政治争议而未经利益博弈的情况下,行政机构的主观性监管很难在管理效率和政治正确之间取得平衡.在此情况下,必须在前端和其他环节嵌入公共参与和公共协商机制,构建协商治理平台,增强政府公共管理活动的政治基础.     

Breaking Into the Olympics

正From b-boys to Olympic athletesWhen they fell in love with breakdancing,or "breaking," these young boys in beanie hats and baggy clothes never could have imagined this fringe cultural offering would one day become a mainstream Olympic sport.Shang Xiaoyu felt star-crossed when breakdancing foot rocked its way into his life.Considered a "bad student" who frequently skipped class and got into fights with other students in junior middle school,this millennial had no idea how his life could,and would,be changed dramatically by a niche dance genre.Shang was 13.It was a regular day at school when he happened to pass through a classroom where a handful of students were practicing their breaking moves.A mere glance of the scene made him stop in his tracks."This looks super cool,"Shang said."I must learn how to do that."