邓小平的发展观探究

邓小平的发展观蕴涵着丰富的内容。从总体上看,主要体现在以下五个方面：提出三个文明建设,谋求总体的全面协调可持续发展;提出共同富裕的构想,探求社会领域内的全面协调发展;重塑民主精神,图求政治领域内的全面协调和以人为本的发展;提出速度、质量与效益的统一,追求经济领域内的协调可持续发展;提出南南合作、南北合作的构想,以求实现国际领域内的全面协调可持续发展。     

论对抽象行政行为的司法审查

在我国立法中排除了人民法院对抽象行政行为的司法审查,但为保护受违法抽象行政为侵害的行政相对人的利益,对抽象行政行为进行司法审查是必要也是可行的。     

群众路线与社会主义新农村建设

党的群众路线明确了社会主义新农村建设的出发点和归宿,明晰了新农村建设的主要依靠力量,厘清了新农村建设的思路和工作方法。坚持党的群众路线是顺利推进社会主义新农村建设的可靠保证,也是全面落实以人为本的科学发展观的生动体现,是建立和谐新农村的有效路径。     

《治安管理处罚法》实施中的初步成效与问题━━对四川省部分地区的调研

《治安管理处罚法》自2006年3月1日实施以来,在违法行为整治、警察权力规范等方面初步显示出其法制效力。但与此同时,也显现出一系列的新情况、新问题,如治安处罚、民警执法、传唤未成年人证人和通知家属等方面都出现了一些新问题,有待《公安管理处罚法》的完善。     

对我国在建设责任政府过程中加强居安思危未雨绸缪能力建设的思考

建设责任政府是我国转变政府职能的重要内容,而要建立责任政府,就不能仅仅关注于对问题的解决及出现问题后的责任追究,它还必须更关注于对问题的预防,对潜在危机的消除。这就必须加强政府居安思危、未雨绸缪的能力建设。为此,政府不仅要提高领导者的相关素质,更重要的是要创新体制和机制,要在建立有效的政策监督、评估机制、建立权责明确的组织体系、加强政策研究机构建设等方面加大力度。     

中华民族精神与和谐社会建设问题研究

深入研究和探讨中华民族精神与和谐社会建设的关系,大力弘扬中华民族精神,为构建社会主义和谐社会服务,是新世纪新阶段党的民族工作必须关注的重要问题之一.     

网络传播伦理原则简论

网络传播的飞速发展给传播伦理带来了巨大的冲击.如何充分认识网络传播的特有规律,建构网络传播中的伦理规范,已成为我国网络传播研究的主要问题.网络环境下媒体伦理的建设要通过社会和媒体及广大网民的共同努力、外部强制和个人自律、道德引导和法律控制的多方位多层次的建构策略,才能建立和完善网络传播伦理,使网络传播良性有效地运行.     

Application of HLA-DRB1 genotyping by oligonucleotide micro-array technology in forensic medicine

The human leukocyte antigen (HLA) system is known to be the most complex polymorphic system in the human genome. Among all of the HLA loci, HLA-DRB1 has the second largest number of alleles. The purpose of this study is to develop an oligonucleotide micro-array based HLA-DRB1 typing system for use in forensic identification, anthropology, tissue transplantation, and other genetic research fields. The system was developed by analyzing the HLA-DRB1 (DRB1) genotypes in 1198 unrelated healthy Chinese Han individuals originating from various parts of China and residing in Shanghai, China. METHOD: Polymerase chain reaction (PCR) coupled with the oligonucleotide micro-array technology was used to detect and type HLA-DRB1 alleles of the sample individuals. The reliability, sensitivity, consistency and specificity were evaluated for use in forensic identification. Furthermore, a meta-analysis was carried out by comparing the allele frequencies of the HLA-DRB1 locus with those of other Chinese Han groups, Chinese minorities and other ethnic populations. RESULTS: All the DNA samples yielded a 273 bp amplification product, with no other amplification products in this length range. The minimum quantity of DNA detected by this method is 15 ng in a PCR reaction system of 25 microl. The population studied appeared to be not in Hardy-Weinberg equilibrium. Observed heterozygosity (Ho), expected heterozygosity (He), expected probability of exclusion (PE), polymorphic information content (PIC), and discrimination power (DP) of the HLA-DRB1 locus from the Shanghai Han ethnic group were evaluated to be 0.8022, 0.8870, 0.7741, 0.8771, 0.9750, respectively. A total of 25 HLA-DRB1 alleles were identified. HLA-DRB1*09XX, *04XX, *12XX and *15XX were the most frequent DRB1 alleles, which were observed in 58.76% of the sample. One hundred and sixteen genotypes were found. The five most frequent genotypes were: *04XX/*04XX (0.0626), *09XX/*09XX (0.0593), *04XX/*09XX (0.0551), *09XX/*15XX (0.0384) and *08XX/*12XX (0.0351). The meta-analysis showed that there were uniquely distributed features of DRB1 alleles among various ethnic populations and among the studied population groups from various regions with the same ethnic origin. CONCLUSIONS: An HLA-DRB1 genotyping system has been developed and established based on the oligonucleotide micro-array technology. The HLA-DRB1 typing of the Han population in Shanghai has revealed a relatively high heterogeneity. Information obtained in this study will be useful for medical and forensic applications as well as in anthropology research. Large-scale micro-array detection is highly accurate and reliable for DNA-based HLA-DRB1 genotyping. These results suggest that HLA-DRB1 DNA polymorphisms and the database of the Shanghai Han group have useful applications in processing forensic casework (as personal identification, paternity test), tracing population migration and genetic diagnosis.     

互动的大众传播与社会流行

大众传播与社会流行的互惠、互利、互制给现行大众传播体系带来了新的挑战,尽快营造良好的大众传播环境,制造"绿色"社会流行氛围已成为有识之士的共识及社会发展之必须。而提高全民审美情趣,加强文化市场管理,把握传播、流行主流是实现大众传播与社会流行健康和谐互动互补的高境界。     

浅析赌博罪认定中的几个疑难问题

赌博罪,一般是指以营利为目的,聚众赌博、开设赌场或以赌博为业的行为。在司法实践中,认定与处理赌博犯罪案件常常会出现一些疑难问题,尤其是随着网络赌博犯罪的出现,关于赌博罪的认定,赌博罪共犯的认定,赌博活动中涉及其他犯罪的处理等问题疑难较多,只按照我国《刑法》的具体规定是远远不够的,应参照2005年最高人民法院、最高人民检察院发布的《关于办理赌博刑事案件具体应用法律若干问题的解释》执行,以更有效地打击和遏制赌博犯罪。