Analysis of sensitivity and specificity: precise recognition of neutrophils during regeneration of contused skeletal muscle in rats

In this report, we applied the TissueFAXS 200 digital pathological analysis system to rapidly and accurately identify neutrophils during regeneration of contused skeletal muscle, and to provide information for follow-up studies on neutrophils to estimate wound age. Rat injury model was established, and skeletal muscle samples were obtained from the control group and contusion groups at 1, 1.5, 2, 3, 4, and 6 h, as well as at 1, 3, 5, and 15 d post-injury (n = 5 per group). The expression of nuclei and neutrophils was detected by hematoxylin and eosin (HE) staining and immunohistochemical (IHC) staining. A total of 20 injury site areas of 0.25 mm² (0.5 mm × 0.5 mm) were then randomly selected at all time points. A TissueFAXS 200 digital pathological analysis system was used to identify the positive and negative numbers. Knowledge of five professional medical workers were considered the gold standard to measure the false positive rate (FPR), false negative rate (FNR), sensitivity, specificity, and area under the curve (AUC) of receiver operating characteristic (ROC) curves. As a result, with a staining area of neutrophils from 8 µm² to 15 µm², the FPR was 4.28%–12.14%, the FNR was 12.42%–64.08%, the sensitivity was 35.92%–87.58%, the specificity was 87.86%–95.72%, the Youden index was 0.316–0.754, the accuracy was 82.80%–88.30%, and the AUC was 0.771–0.826. The AUC was largest when the cut-off value of the staining area was 12 µm². Our results show that this software-based method is more accurate than the human eye in evaluating neutrophil infiltration. Based on the sensitivity and specificity, neutrophils can be accurately identified during regeneration of contused skeletal muscle. The TissueFAXS 200 digital pathological analysis system can also be used to optimize conditions for different cell types under various injury conditions to determine the optimal cut-off value of the staining area and provide optimal conditions for further study. Furthermore, it will provide evidence for forensic pathology cases.     

当前世界经济走势及其对中国的影响

今年上半年,在美国次贷危机继续发酵和油价暴涨等诸多因素的综合影响下,世界主要经济体和全球经济趋缓,通货膨胀加剧。由于困难因素远未缓解,美国经济低速增长还将持续一段时间,发展中国家经济将继续受到一定的拖累。在当前不确定的国际经济金融环境下,中国发展的挑战与机遇并存,只要积极、及时、灵活应对,就能趋利避害,使经济继续保持强劲发展势头。     

Responsibility Determination: Free Will and Legal Responsibility in the Age of Artificial Intelligence

Free will is the foundation of determination of responsibility. Genetic enginnering represented by technologies of gene editing, artificial medical devices and AI have fundamentally challenged the concept of free will and so have significantly influenced determination of legal responsibility. These challenges are fundamental, not instrumental, and can be divided into two aspects in legal philosophy. First, the direct challenge, that is, the emerging technology represented by genetic engineering and artificial narrow intelligence (ANI) has challenged the concept of free will. Second the would-be ultimate challenge, that is, presented by an artificial general intelligence (AGI) agent that is considered to reach humanlevel free will, can be a legal subject, thus taking full legal responsibility. The direct challenge constitutes a new “forgiveness” condition for taking responsibility. The would-be ultimate challenge deserves significant attention, because the concept of free will is not only about human responsibility, but also about human dignity.     

中国证券仲裁制度构建之探讨

本文分析了中国构建证券仲裁制度的可能性,并结合发达国家的实践确定了我国证券仲裁应采用的模式,并设计了专门的证券仲裁程序。     

发挥优势,为构建社会主义和谐社会服务

本文论述了当前构建社会主义和谐社会的重大意义以及维护稳定与构建和谐社会的辩证关系,并进一步探讨了民进发挥自身优势,为构建和谐社会服务的途径和方法。     

易地扶贫搬迁社区的社会稳定风险防范与化解

易地扶贫搬迁作为脱贫攻坚的重要手段,既要做到搬迁群众"搬得出",更要保证其"稳得住""能致富".易地扶贫搬迁主体利益的多元性和复杂性决定了必须要重视搬迁社区治理.易地扶贫搬迁社区社会稳定风险防范与化解是搬迁社区社会治理的重要内容,要明确"防范什么""谁来防范""怎样化解"三大重点任务,同时要从构建长效治理机制、精准识别...     

农村民间组织在构建农村和谐社会中的价值功能和关键点

农村民间组织在组织农民参与国家和社会事务的管理、参与社会主义新农村建设及促进农村社会和谐方面正发挥着越来越大的作用。必须充分认识农村民间组织的价值功能,抓住农村民间组织发展面临的重大机遇,规范发展农村民间组织,提升农村民间组织在构建农村和谐社会中的作用．促进农村经济社会发展。     

中国合作性外交及其目的分析——一个历史的视角

本文通过历史和现实三个时段（封贡体系时期、新中国建立至改革开放前、改革开放至今）的中国外交分析,得出两点结论：第一,自古代社会起至今,合作而非对抗是中国对外行为的基本选择。中国积极寻求与周边、地区和世界的和平与合作。第二,中国合作性外交的目的在于实现国际秩序和世界正义的统一。本文认为,封贡时期的东亚秩序本身就是正义的,维持秩序就是维持正义,中国在封贡时期的国际合作保证了秩序与正义的统一;新中国建立后到改革开放前,中国以和平共处五项原则为基础与各国发展友好合作关系,坚定维护国际关系基本准则和基本秩序,同时又通过反霸反帝反殖斗争和建立国际经济新秩序追求世界正义;改革开放后的中国,全面融入国际社会,参与国际组织,遵守国际规范,与世界各国发展全方位合作关系,同时又通过反霸斗争、建立国际政治经济新秩序以及建设“和谐世界”推动世界正义的实现。     

马动脉炎病毒的血凝性研究

在RK-13细胞上培养的马动脉炎病毒(Equine viral arteritis,EAV)在4℃、室温和37℃条件下,用多种动物的红细胞进行血凝试验,病毒在不同温度下均可凝集小鼠和鸡的红细胞,但不能凝集牛、绵羊、山羊、马、猪、豚鼠、仓鼠、兔及鹅的红细胞.将病毒用吐温-80和乙醚处理后,血凝活力会显著增强,其HA效价比未经处理的病毒高4-8倍,且血凝像更加清晰.处理病毒的最适条件是在冰浴状态下用终浓度0.6-1.25 mL/L吐温-80振荡处理15-60 min,再加1/2体积的乙醚振荡作用5-15 min.     

技术许可中职务发明人权益的保护研究——兼析职务发明人的优先受让权

随着科技创新的重要性在社会生活中日益凸现,各国都在积极采取各种措施激励科技创新、加快技术转化.在科技创新主导的知识产权战略中,科技人才的创造力起着决定性作用.