模板DNA用量对荧光STR复合扩增检测的影响

目的 探讨模板DNA用量对荧光STR复合扩增检测的影响,寻求荧光STR复合扩增检测的最适扩增模板DNA用量。方法 采用模板DNA(9947A)不同的扩增用量,对Profiler Plus试剂盒的基因座进行扩增,在3100型全自动遗传分析仪上作了检测。结果 3100型遗传分析仪检测的最适扩增模板DNA用量在0.31～2.5ng之间。结论 模板DNA量过高或过低均会影响荧光STR复合扩增检测结果的可靠性。     

固相萃取同时提取尿中的39种药毒物

目的建立固相萃取方法同时提取尿中的39种药毒物并用高效液相色谱法进行分析。方法以多沙普仑为内标,1ml尿样经Oasis小柱固相萃取,用HPLC进行分析。结果39种药毒物可同时从尿中提出,内源性物质不干扰测定。其绝对回收率除吗啡外均大于70％;天内及天间精密度均小于10％;检测限1-15ng／ml;线性相关系数在0.9977以上。结论本法快速、简便、重现性好,空白干扰小,可用于实际案例的药物毒物筛选。     

上海市城市轨道交通事故死亡案例分析

目的分析城市轨道交通事故死亡案例特点,为今后此类事故的法医学鉴定以及预防提供参考。方法收集上海市2005--2008年间城市轨道交通事故死亡案例18例,从案件的性质、损伤机制及特征等方面,总结此类案件的法医学特点。结果18例城市轨道交通事故死者,年龄在21～55岁。其中,自杀14例,意外4例;颅脑损伤死亡14例,创伤性休克死亡4例。损伤以撞击、摔跌伤为主,部分存在碾压伤。结论城市轨道交通事故所致损伤具有其特征性,案件性质主要为自杀,部分为偶然的意外事件,具有可防范性。     

Preliminary Studies on the Absorbance Ratio Method Used to Determining the Age of Stamp‐pad Ink Seal

Stamp‐pad ink seals are often placed on important documents, and determining the dating of stamp‐pad ink seal is important to assess the authenticity of a document. In this study, 20 different brands and types of stamp‐pad inks were classified by ultraviolet visible spectrophotometer. Six different brands and types of papers were classified by micro‐Fourier transform infrared spectrometer and visual spectral comparator 6000. Three different brands of stamp‐pad inks and two different types of paper were used to make diachronic samples of the seals. A method was employed to date the stamp‐pad seals by comparing absorbance ratios and inks of known age. Curves were created to show the relationship between the absorbance ratio of ink and the age of the seals. The experimental results showed that the absorbance ratio method was applicable for the relative dating of stamp‐pad ink seals in some cases where the seal was placed on the document within 10 weeks.     

Faux Blood Fingermark on Pistol: Latent Fingerprint Developed by Whole Blood

We present a case of a faux blood fingermark, wherein a natural latent fingerprint on the pistol at the crime scene was enhanced by the whole blood of the victim. A male was shot to death. The crime scene investigator did not find any blood fingermark during the collection of evidence, but a blood fingerprint was noticed in the firearms examination. We identified that the fingerprint was of the victim; a series of experiments on the nonporous substrate were then conducted; the death in this case was classified as a suicide. The phenomenon is interesting, and thus, calls for attention of the forensic community.     

Performance Evaluation of a Registered Ballistic Database Using the Evofinder® System

Firearms for police in China are registered along with their fired bullets and cartridge cases. A Registered Ballistic Database (RBD) of 1000 Norinco QSZ‐92 pistols with registered ammunition was established and was evaluated through the Evofinder® system. In this research, 1000 bullets and 1000 cartridge cases were randomly selected and correlated against an RBD of 2996 bullets and 2999 cartridge cases. Examiners found that successful identifications all ranked 1st, supported with land (100%), groove (97%) engraved areas, and primary marks (85.6%) for bullets, and firing pin impressions (99.8%), and breech face marks (99.9%) for cartridge cases. Two known matches (KM) for the same pistol rank in the top two (100%). The distribution of similarity scores varies from marks; however, the Evofinder® system could still effectively distinguish known matches from known nonmatches (KNM) for either bullets or cartridge cases. This study demonstrates the efficiency of the RBD.     

Efficiency Analysis of EX16 +10Y Kit on Detection of the Uygur Population in Xinjiang ProvinceCSCD

Objective: To analyse the efficiency of EX16+10Y kit on the forensic detection of the Uygur in Xinjiang province. Methods: The blood samples were extracted from 4 620 male individuals of Uygur in Xinjiang province, and amplified by EX16+10Y kit. The typing of amplification products was performed by 3130xl genetic analyzer. Results: The genotyping graphs of 15 autosomal STR loci and 10 Y -chromosomal STR loci from 4 620 male individuals of Uygur in Xinjiang province were acquired completely. The genotype distribution of 15 autosomal STR loci was consistent with Hardy-Weinberg equilibrium. The heterozygosity, polymorphism information content and discrimination power of STR loci were 0.637-0.838, 0.580-0.860 and 0.811-0.978, respectively. There were 766 haplotypes in 10 Y -chromosomal STR loci. Conclusion: The test results of EX16+10Y kit is accurate and trustworthy, which can simultaneously be used for the individual identification and the screening of paternal pedigree in practical work. © 2018 by the Editorial Department of Journal of Forensic Medicine.     

机体死亡后组织细胞DNA含量与死亡时间推断

早期死亡时间的推断方法有许多,但近年来通过测量组织细胞DNA含量的变化规律来推断早期死亡时间越来越引起人们的注意。本文就常见的细胞DNA含量的检测方法、变化规律及其在实践中的应用情况加以论述,并对不同检测方法和实际应用效果、前景加以评定。     

上海地区干尸形成的条件及机理

运用常规的尸体检验方法对干尸进行检验,根据季节、现场、尸体保存方法等探讨干尸形成的条件和机理。发现在上海温暖潮湿南方地区,干尸形成多见于气温较低、环境干燥季节,死亡前多有脱水和绝食经历,且伴有翻动尸体及通风等人为干预因素。     

SPE-HPLC分析全血中37种药（毒）物

目的建立全血中37种常见药(毒)物的固相萃取-高效液相色谱(SPE-HPLC)分析方法。方法以多沙普仑为内标,0.5mL全血经Oasis小柱固相萃取后,用HPLC进行分析,内源性物质不干扰测定。色谱柱采用LiChrospher!100RP-C18柱(250mm×4.0mm×5μm);二极管阵列检测器,检测波长为230nm和250nm,同时进行紫外扫描;柱温50℃。结果37种药(毒)物的绝对回收率除吗啡外均大于61.68%;日内及日间精密度均小于10%;检测限1～30ng/mL;线性相关系数在0.99798以上。结论本法快速、灵敏、重现性好,可用于实际案例中多种药(毒)物的分析。