The Space Between: Demonization of Opponents and Policy Divergence

Prior research on policy conflicts indicates a tendency among policy actors to misperceive the influence of actors engaged in policy debates based on the degree of distance between their relative policy positions. This research develops a measure for assessing the degree and direction of the misperception effect. This measure is then utilized as a dependent variable to assess the relationship between theoretically relevant factors and the degree to which actors will exaggerate the influence of their opponents and allies. The research uses original survey data of policy actors engaged in the debate over hydraulic fracturing in New York. The results indicate misperceptions of relative influence are prevalent and most associated with the experience of a policy loss and holding relatively extreme policy beliefs. The findings provide new insight into factors that influence the demonization of political opponents. These insights are timely in the context of polarized debates over environmental and energy policy in the United States.     

Interview with AbbyTC5: a pioneering ‘HomeGirl’ in Hip-Hop herstory

Jessica N. Pabón interviews graffiti artist Abby Andrews, a.k.a. AbbyTC5, about her canvas painting Queens, the image on the front cover of the special issue.     

猪生殖与呼吸综合征病毒CH-1a株GP5蛋白基因的表达及其单克隆抗体的制备

将猪生殖与呼吸综合征病毒(PRRSV)CH-1a株GP5蛋白基因信号肽部分删除后克隆于pGEX-6p-1载体上,并在大肠埃希氏菌中进行表达。经SDS-PAGE电泳分析发现,融合表达的rtGP5蛋白约42 ku,将融合蛋白rtGP5用PRRSV阳性血清进行Western-blotting分析,证实所表达的融合蛋白能被其特异性识别。收获融合表达产物rtGP5,按50μg/只的剂量与等量弗氏佐剂乳化,经腹腔免疫BALB/c小鼠3次后,取脾细胞与SP2/0骨髓瘤细胞进行融合,分别以融合蛋白rtGP5和GST蛋白为抗原,通过间接ELISA方法对融合细胞的上清液进行检测,筛选阳性克隆,结果得到了15株能稳定分泌抗rtGP5蛋白抗体的阳性细胞克隆株。经间接免疫荧光检测发现,获得的15株单克隆抗体都能与PRRSV感染细胞产生特异性荧光。15株单克隆抗体亚型鉴定结果显示均为IgG1型,且轻链均为κ链。     

葡萄球菌GST-mTSST-1融合蛋白抗体间接ELISA检测方法的建立

以纯化的金黄色葡萄球菌无毒诱变超抗原GST-mTSST-1融合蛋白作为检测抗原,通过优化ELISA反应条件,初步建立了检测血清特异性GST-mTSST-1抗体的间接ELISA方法,监测了小鼠血清中特异性GST-mTSST-1抗体水平的动态变化规律。方阵试验确定的GST-mTSST-1抗原的最适包被浓度为5.0μg/mL,血清最佳稀释倍数为1∶100,ELISA阳性反应的临界值为D490 nm≥0.219,批内和批间重复试验的变异系数均小于10%。应用该方法对试验小鼠血清进行检测,结果表明,建立的间接ELISA方法具有较高的敏感性、特异性和较好的重复性,适用于检测特异性GST-mTSST-1血清抗体。     

充分发挥兴安在大桂林旅游圈的作用

兴安是大桂林旅游圈一颗璀璨的明珠,应当以建设试验区为契机,拉长桂林旅游的链条,充分发挥兴安在大桂林旅游圈的作用：引导游客探寻漓江源头的神奇和美丽;来兴安进行文化寻根访迹;上猫儿山体验瑶族风情;体验兴安的农家乐休闲旅游;开展工业旅游;进行高尔夫休闲度假游;举行有纪念意义的婚礼;在兴安休闲度假养生养老。     

关于一类半无限多目标规划离散型算法的偏差估计

本文将文献[5]中的偏差估计公式推广到有任意多个目标函数和α∈(0, ∞)的情形并给出约束参数集维数可任意的数值例子.     

中药外敷对哮喘豚鼠肺组织IL-5基因表达的影响

目的:探讨中药循经敷贴治疗哮喘的作用机制。方法:采用Hutson法复制豚鼠模型,治疗组隔日诱喘后用中药敷贴30 m in,对照组隔日诱喘后采用9.0 g/L氯化钠注射液浸润纱布敷贴,比较记录诱喘潜伏期时间。两周后处死豚鼠,取肺组织,用半定量RT-PCR法比较IL-5 mRNA的表达。结果:中药循经敷贴能显著延长豚鼠诱喘潜伏期,下调哮喘豚鼠肺组织IL-5 mRNA的表达。结论:抑制炎性细胞因子IL-5 mRNA的表达可能是中药循经敷贴治疗哮喘的重要机制之一。