AFLP分子标记技术的新进展及其在法医植物学中的应用

扩增片段长度多态性(amplified fragment length polymorphism,AFLP)是一种用来检测基因组多态性的新一代分子标记,具有分辨率高、稳定性好、重复性好等特点.近年来,研究人员对该技术进行了不断的优化和完善,并由之衍生出多种相关技术.AFLP技术在动物、植物及微生物等许多研究领域已有广泛应用,在法医植物学中得到初步发展并成为研究热点.本文主要介绍了AFLP技术的新进展以及在法医植物学中的应用情况.     

滥用药物体内代谢的研究进展

毒品在体内尿苷二磷酸葡醛酸转移酶、细胞色素P450、羧酸酯酶、磺基转移酶、丁酰胆碱酯酶、儿茶酚-O-甲基转移酶和吗啡6-脱氢酶等代谢酶催化下,发生葡萄糖醛酸化、水解、氧化、磺酸化等多种反应,生成有活性或者无活性的代谢产物,然后经尿液、胆汁或者其他途径排出体外。不同毒品的代谢途径不同。本文介绍了近年来吗啡类、苯丙胺类、氯胺酮、大麻类和可卡因等毒品在人和动物体内的代谢研究,综述了这些毒品体内代谢的代谢部位、代谢酶、代谢产物及其生理活性的研究进展。     

兔阴茎海绵体平滑肌细胞培养及其RyRs的表达

目的探讨兰尼碱受体(RyRs)在兔阴茎海绵体平滑肌细胞(CCSM)的正常表达。方法取雄性性成熟新西兰兔CCSM细胞,用组织块法进行细胞培养,差速贴壁法进行细胞纯化,利用倒置显微镜观察、免疫荧光染色法鉴定细胞纯度;取三代以内培养细胞,通过Genebank查出RyRscDNA序列,针对RyRs3种不同亚型的非同源部分用PrimerPremier5.0软件设计3对引物,以逆转录PCR方法分别检测RyRs3种亚型mRNA的表达,凝胶成像系统分析结果。结果培养7d开始有细胞从组织块长出,15～20d后长满瓶底,呈典型的“峰-谷”现象,经倒置显微镜观察见培养的细胞呈现典型的梭形,免疫荧光染色可见细胞内染为绿色的肌丝,证实培养的细胞为CCSM细胞,经差速贴壁法纯化后,细胞纯度可接近100%。经RT-PCR检测,CCSM只表达RyRs1,未见RyRs其余亚型的表达,提示CCSM表达RyRs1蛋白。结论CCSM表达RyRs中的1亚型,提示其可能参与CCSM细胞的钙调节,进而能调节阴茎勃起功能。     

警察任务法治化之困境及出路

警察任务在警察法治化构建过程当中的地位十分重要,《中华人民共和国人民警察法》第2条有关警察任务的规定在实际操作过程中明显过于宽泛,又受到政治因素的干扰,引起众多对于警察职责职权方面的争议,甚至在某种程度上造成警察在执法中进退两难的境地。警察任务如何执行,既关乎人民生命财产安全、社会秩序健全,更影响到国家在法治化建设进程中如何适当脱离"人治",入轨"法治"的转型。对此,需要通过对当前我国警察任务相关规定的拆解,分析时下警察任务所面临的现实困境,找到问题的根源,结合法治化进程明确警察任务的规范化途径。     

Overreaching In Registered Land Law

Beneficial interests under a trust were not intended to be overriding interests under section 70(1)(g) of the Land Registration Act 1925. The position was altered by Williams & Glyn's Bank Ltd v Boland , which determined that an interest under a trust for sale would bind a purchaser if the beneficiary were in actual occupation. The decision raised the question whether such interests could be overreached once the beneficiary was in occupation of the trust property. City of London Building Society v Flegg held that the relevant beneficial interest had been overreached. Both decisions assume that overreaching in registered conveyancing takes effect as it does in unregistered land. Yet there is considerable evidence that the Land Registration Act contains its own overreaching machinery. The House of Lords applied the wrong overreaching provisions in Boland and Flegg and there is no legal basis on which to recognise that trust interests can override a subsequent disposition under section 70(1)(g).     

论我国社会保险立法的新理念与新使命——以《社会保险法》为主要分析样本

社会保险是事关民生的重大制度安排。面对我国社会保险制度改革前所未有的新形势、新课题和新考验,社会保险立法应当秉承新的立法理念,承载新的历史使命。其中,坚持《社会保险法》是劳动者的权益法和福利法,建立与时俱进的发展型社会保险制度,重构国家在社会保险中的角色与义务,推动新型劳资关系与社会主义和谐社会的建设,是社会保险立法应当解决的核心问题。     

Another type of ‘Other’ in EU Law? AB (2) MVC v Home Office and Rahman v Secretary of State for the Home Office

EU citizenship law has to date paid little attention to the extended family members of Union citizens, a group mentioned just once in Citizenship Directive 2004/38. This note suggests that the current EU legal framework gives too much discretion to the member states, providing scope for the rights of EU migrant workers to be breached with impunity. It also questions whether the new mechanisms for addressing misapplication of EU law are robust enough to hold national authorities to account for their treatment of other family members.     

丽蝇科蝇类的生物学特性及其法医学应用

双翅目（Diptera）丽蝇科（Calliphoridae）蝇类是最早出现在腐败尸体、尸块上的主要蝇类。在法医昆虫学领域,根据丽蝇科的生活特性,可以较准确地推断出凶杀案或无名尸案的死亡时间（postmorteminter．val,PMI）,为案件侦破提供有利线索。本文介绍了丽蝇科蝇类的生物学、形态学特征,并对其在法医昆虫学与分子生物学、数学形态学、法医毒理学方面的联合应用进行了综述。     

氯化琥珀胆碱分析测定的研究进展

氯化琥珀碱（suxamethoniumchloride）是一种去极化类肌肉松弛药,临床用作全身麻醉时的肌肉松弛剂．使用过量可引起心动过缓、心律失常、心搏骤停、甚至死亡等不良反应。本文综述了利用红外光谱法、化学显色反应法、滴定法、酶电极法以及色谱和质谱法等方法对氯化琥珀胆碱进行分析测定的研究进展。     

Autonomy,Reflexivity, Tragedy: Notions of Democracy in Camus and Castoriadis

Abstract

This paper looks at two 20th century theories of tragedy: those of Cornelius Castoriadis and Albert Camus. The theories that each proffer of this ancient cultural form are striking. Against more standard views, both theorists stress that tragedy is a cultural form that has only arisen historically in cultures whose forms of religious thought have been laid open to question. In this way, both argue that tragedy is an important democratic cultural form, which stages the confrontation between a no longer unquestionable divine order, and human autonomy. The intent of the paper, from the start, is a political one. It wants to place Camus alongside Castoriadis as a ‘post-Marxist’ thinker, who belongs meaningfully to what Dick Howard has called ‘the Marxian legacy’. More than this, it aims to do this by staging Camus' theorisation of tragedy, with Castoriadis', as a powerful riposte to the conservative criticism of democracy as a modern political form, that is, that it cannot muster sacral cultural forms forceful enough to meaningfully unite people beneath its banner.