The Class Characteristic Mark of the H&M Mul‐T‐Lock Picking Tool in Toolmarks Examination

Mul‐T‐Lock is a high security lock cylinder distinguished by the use of a telescoping “pin‐in‐pin”—tumbler design. Picking the Mul‐T‐Lock cylinder with a traditional picking tool is highly complicated because it can get stuck between the inner and outer pins. The H&M Mul‐T‐Lock picking tool was designed to overcome this problem and facilitate the picking of the “pin‐in‐pin” cylinder. The purpose of this research is to determine whether H&M Mul‐T‐Lock picking tool leaves class characteristic mark and whether it can be distinguished from traditional picking tools marks and from regular key marks. It also describes and determines the class characteristic mark left on telescopic pins, its origin, recurrence, and its benefit to the toolmarks examiner. When receiving a Mul‐T‐Lock from a crime scene, a toolmarks examiner can quickly determine whether or not it was picked by an H&M Mul‐T‐Lock picking tool by noticing the class characteristic mark which this typical tool leaves.     

猪囊尾蚴T24基因的克隆与表达

采用RT-PCR方法扩增猪囊尾蚴T24免疫原基因,将扩增产物与pGEM-T easy载体连接,重组质粒经PCR、酶切鉴定后进行测序;构建T24基因的pGEX-4T-1原核表达载体,经IPTG诱导表达后,进行SDS-PAGE、Western-blotting;用所表达的蛋白免疫小鼠,经ELISA检测血清抗体,验证其免疫原性。结果显示。所克隆的T24基因片段长716 bp。含有1个678 bp的开放阅读框,其编码226个氨基酸,与已报道的猪囊虫T24基因核苷酸序列同源性为100％;表达的融合蛋白大小为40 ku,并能被猪囊虫阳性血清识别;免疫小鼠在免疫1周后即可检测到血清抗体,第30 d达到较高水平,表明该融合蛋白具有较好的免疫原性。     

Use of Cardiac Injury Markers in the Postmortem Diagnosis of Sudden Cardiac Death

In the daily practice of forensic pathology, sudden cardiac death (SCD) is a diagnostic challenge. Our aim was to determine the usefulness of blood biomarkers [creatine kinase CK‐MB, myoglobin, troponins I and T (cTn‐I and T), and lactate dehydrogenase] measured by immunoassay technique, in the postmortem diagnosis of SCD. Two groups were compared, 20 corpses with SCD and 8 controls. Statistical significance was determined by variance analysis procedures, with a post hoc Tukey multiple range test for comparison of means (p < 0.05). SCD cases showed significantly higher levels (p < 0.05) of cTn‐T and cTn‐I compared to the control group. Although only cases within the first 8 h of postmortem interval were included, and the control group consisted mainly of violent death cases, our results suggest that blood troponin levels may be useful to support a diagnosis of SCD.     

海上保险委付研究

委付是海上保险中的一项重要制度,但各国规定有所不同。我国《海商法》中相关规定有待完善和明确。委付只适用于推定全损;委付行为是单方行为,属于要约;委付的成立必须有明确的意思表示,且不得附带条件;委付一经保险人接受,不得撤销,而不是撤回。     

口蹄疫病毒3C蛋白酶的T4噬菌体表面展示

对FMDV 3C基因克隆质粒p3C-T进行PCR扩增,获得了口蹄疫病毒3C蛋白酶基因片段,将此片段与T4噬菌体整合质粒pR的EcoRⅠ酶切片段连接,转化E．coli,DH5α工程菌,经EcoRⅠ酶切、质粒PCR扩增、插入方向筛选及测序鉴定,成功获得了T4噬菌体重组整合质粒pR- 3C。将其转化E．coli E2后,与SOC基因缺失的噬菌体φT4-Z1同源重组,经溶菌酶依赖性筛选,获得了快速溶菌型噬菌体φT4-3C。经噬菌体PCR扩增、SDS-PAGE分析和Western-bot分析,重组噬菌体可展示约26 ku的重组蛋白;其大小与预期相符,具有免疫原性。     

邓小平科技思想的理论蕴涵探析

邓小平科学技术思想是涵盖科技观、人才观、动力观和功能观等内涵丰富、逻辑完整的理论体系。邓小平科技思想理论蕴含中四观科学阐释了什么是科学技术,怎样对待和应用科学技术的问题,深入研究和准确把握邓小平科技思想的理论蕴涵,既有重要的理论意义,又有突出的实践价值。     

松果腺对大鼠脾脏T淋巴细胞增殖率季节性变化的影响

目的:探讨松果腺对脾脏免疫功能季节性变化的影响。方法:采用松果腺摘除动物模型,运用噻唑蓝(MTT)比色分析法,测定冬春两季Wistar大鼠脾脏T淋巴细胞增殖率变化情况。结果:春分时生理组、伪手术组和手术组脾脏T淋巴细胞增殖率均低于冬至时相应组别的数值,差异具有显著性(P<0.01);冬至时生理组与伪手术组脾脏T淋巴细胞增殖率比较,差异具有显著性(P<0.01);春分时,手术组与伪手术组脾脏T淋巴细胞增殖率比较,差异具有显著性(P<0.01);无论冬至或春分,伪手术组和手术组脾脏T淋巴细胞增殖率均低于生理组,其中手术组数值明显低于当季生理组,差异具有显著性(P<0.01)。结论:脾脏T淋巴细胞增殖率存在冬高春低的变化趋势,松果腺摘除对脾脏免疫功能具有下调作用,松果腺对脾脏T淋巴细胞增殖率的影响不具有唯一性。     

毒害艾美球虫二次感染雏鸡外周血液免疫功能的变化

将180只14日龄雏鸡随机分为毒害艾美球虫(Eimeria necatrix)初次感染组、二次感染组和对照组,应用免疫SPA菌体花环、间接ELISA及细胞培养技术和四甲基偶氮唑盐(MTT)测定法对相关免疫学指标进行了检测,以研究毒害艾美球虫二次感染对雏鸡外周血液免疫功能变化的影响。结果显示,毒害艾美球虫二次感染雏鸡外周血液T、B淋巴细胞数量及其对ConA或PMA的增殖反应和血清IgG、IgM、IgA免疫球蛋白含量均不同程度地高于未感染毒害艾美球虫的对照组雏鸡。证实毒害艾美球虫二次感染雏鸡外周血液的细胞免疫和体液免疫功能均明显提高。     

新城疫病毒HN基因与T4噬菌体SOC基因在大肠埃希氏菌中的融合表达

用RT PCR方法扩增了新城疫病毒 (NDV)的完整HN基因序列 ;设计了 1对带有EcoRⅠ限制位点的引物 ,用PCR扩增出了HN基因片段。将该片段克隆至 pSOC质粒SOC基因 3′端 ,成功构建了重组质粒pSOC HN。用该重组质粒转化E .coliBL 2 1(DE3)感受态细胞 ,以终浓度 1mmol/mL的IPTG诱导表达。在SDS PAGE凝胶上可检测到分子质量约 6 7ku的特异条带 ,蛋白质印迹法证实 ,表达产物与NDV抗血清具有良好的反应性