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171.
172.
Screening Test for Shed Skin Cells by Measuring the Ratio of Human DNA to Staphylococcus epidermidis DNA 下载免费PDF全文
Hiroaki Nakanishi Ph.D. Takeshi Ohmori Ph.D. Masaaki Hara Ph.D. Shirushi Takahashi M.D. Ph.D. Akira Kurosu M.D. Ph.D. Aya Takada M.D. Ph.D. Kazuyuki Saito M.D. Ph.D. 《Journal of forensic sciences》2016,61(3):618-622
A novel screening method for shed skin cells by detecting Staphylococcus epidermidis (S. epidermidis), which is a resident bacterium on skin, was developed. Staphylococcus epidermidis was detected using real‐time PCR. Staphylococcus epidermidis was detected in all 20 human skin surface samples. Although not present in blood and urine samples, S. epidermidis was detected in 6 of 20 saliva samples, and 5 of 18 semen samples. The ratio of human DNA to S. epidermidisDNA was significantly smaller in human skin surface samples than in saliva and semen samples in which S. epidermidis was detected. Therefore, although skin cells could not be identified by detecting only S. epidermidis, they could be distinguished by measuring the S. epidermidis to human DNA ratio. This method could be applied to casework touch samples, which suggests that it is useful for screening whether skin cells and human DNA are present on potential evidentiary touch samples. 相似文献
173.
目的研究德州市水域中硅藻分布的特点,建立德州市春季和夏季的各水域硅藻数据库。方法对德州市主要水域的130个取水地点进行春季和夏季两次采样,用强酸消解-真空抽滤-扫描电镜法检测各水域中硅藻的种属及含量,统计分析水域中硅藻含量、群落组成、相对丰度和优势种属特点。结果夏季的硅藻含量高于春季的硅藻含量,河流两个季节硅藻含量无统计学显著差异(P>0.05),湖泊和水库两个季节硅藻含量存在统计学差异(P<0.05)。各水域之间硅藻含量存在差异,含量最高为608020个/10mL,最少为10个/10mL。各水域之间种属组成、优势种属和种属优势率存在差异。结论德州地区不同水域、不同季节的水中硅藻种属和含量具有明显的特异性;建立硅藻数据库和掌握本地区水域中硅藻种属特点,有助于德州市水域内水中尸体案件硅藻的检验和落水地点的判断。 相似文献
174.
目的:探讨氨基比林血痕预试验处理血痕后样本DNA含量的变化及对STR分型检测的影响。方法10名健康无关个体EDTA抗凝血液制成滤纸血痕,氨基比林血痕预试验检测,按试验后血样干燥保存时间分30 min、1 h、3 h、6 h、12 h、24 h共6个实验组,并采用磁珠法、QIAcube DNA纯化法、Chelex-100法三种方法提取样本DNA,应用荧光定量PCR检测样本DNA含量,PCR-STR荧光技术进行STR分型。结果提取方法相同时,氨基比林血痕预试验后血样随干燥保存时间的延长,样本DNA含量呈逐渐降低的趋势。保存时间相同时,不同DNA提取方法间,样本DNA含量差异也有统计学意义。90.56%样本均可获得16个STR基因座明确分型。结论氨基比林血痕预试验对血痕样本DNA有损伤,24 h内多可获有效STR分型。磁珠法提取样本DNA进行STR分型,效果最好。 相似文献
175.
Yasuhisa Seo Ph.D. Daisuke Ichida Shingo Sato Kohji Kuroki Tetsuko Kishida M.D. Ph.D. 《Journal of forensic sciences》2014,59(3):779-784
In order to devise a better forensic test for diatoms, the DNA binding ability of the diatom frustule constructing by silica, in the presence of chaotropic ions were utilized. It was proved that the diatoms were able to be captured via λDNA using silica‐coated magnetic beads (Mag beads), followed by isolation and purification from the Mag beads as a solid phase by substituting the chaotropic agent with ultrapure water. Five cases of drowning, three in freshwater and two in seawater, were applied to the present method and similar results as the usual diatom test were obtained. Specimens of lung and other organs were rendered clearly visible, with elimination of foreign impurities. The present method appears applicable for detection of diatoms indirectly using PCR amplification of bound DNA or directly staining of the DNA. 相似文献
176.
Clive R. Hollin 《心理学、犯罪与法律》2013,19(1):85-86
Abstract G. H. Gudjonsson and L. R. C. Haward, Forensic Psychology: A Guide to Practice. London. Routledge, 1998. 相似文献
177.
《Forensic Science International: Genetics Supplement Series》2013,4(1):e73-e74
In order to increase the information about Indels, we report allele frequencies and statistical parameters of forensic efficiency obtained typing a sample of 114 unrelated healthy individuals living in San Basilio de Palenque – Colombia using a panel of 38 autosomal InDels. No significant deviations from Hardy–Weinberg expectations were found except in the marker rs10629077 (p = 0.0002). The present database will be useful for forensic and paternity purposes for the region studied. Moreover, these additional markers can help forensic laboratories to solve parentage testing as well as to improve the analysis of degraded DNA samples. 相似文献
178.
Lei Guang 《The Pacific Review》2013,26(3):399-422
This article examines Russian Government policy towards Northeast Asia as an interplay between aspirations, which have been expressed by government leaders, parliamentary figures and prominent academics and journalists and actual results. The article uses three levels, global, regional and bilateral, as a basis for the analysis. In terms of global interests the Russian leadership has sought diplomatic balance against the US, in terms of regional interests the leadership has pursued the aim of economic and security integration while at the bilateral level Russian leadership has sought specific benefits from particular relations with China, Japan and South Korea. The article notes that Russia has obtained certain diplomatic benefits from the relationship with China which serve its interests at the global level. At the regional level policy aspirations have been frustrated by Russia's dire economic condition and the tendency to be treated as an ‘outsider’. Russia has benefited from an improvement of bilateral relations with China, Japan and South Korea but these gains do not translate into an improved position at the regional level yet. while the Russian leadership has been nurturing the development of bilateral relationships problems particular to those relationships hinder its wider regional acceptance. 相似文献
179.
建设闽台共同市场,是加强两岸交流与合作的重要途径。从长远看,闽台共同市场作为两岸共同市场的试点,其建设和发展必将推进海峡两岸以共同市场为纽带的经济合作制度化。文章在分析闽台加强经贸合作的战略需求的基础上,阐述了构建闽台共同市场的条件与基础,提出了闽台共同市场的基本原则、运行机制和重点内容,并探索了实现这一构想的关键模块及相关对策。 相似文献
180.
Sibship Analysis Based on Parental Genotype Reconstruction from Any Number of Reference Siblings 下载免费PDF全文
Previous analyses of alleged siblings have calculated the conditional probability of a test sibling's genotype given a reference sibling's genotype; however, more genetic information is available when two or more reference siblings are tested. Based on the root concept of identity by descent (IBD), we present a sibship method of parental genotype reconstruction (PGR) that can use any number of reference sibs. Nine PGR patterns, along with their respective IBD proportions of full‐sibs and half‐sibs, were identified and used to calculate joint sibship probabilities and likelihood ratio formulas. In addition, a correction PGR was developed for situations involving the same genotype repeating in the reference sibs. The method is simple and can be applied to any number of alleged full or half‐siblings. 相似文献