5F-UR-144的热不稳定性研究

目的利用气相色谱-质谱法(GC-MS)、液相色谱-四级杆-飞行时间质谱(LC-Q-TOF/MS)和核磁共振光谱法(NMR)研究合成大麻素5F-UR-144遇热分解的具体变化情况。方法对照品用无水乙醇定容稀释后,经GC-MS和LC-Q-TOF/MS检测得到对应的色谱图和质谱图;对照品分别于常温和280℃密封加热后用无水乙醇定容稀释,经LC-Q-TOF/MS和NMR检测得到对应的质谱图、¹H和¹³C核磁共振波谱;对照品分别于常温逐步提升至300℃密封加热后用无水乙醇定容稀释,经LC-Q-TOF/MS检测得到对应的色谱图。结果5F-UR-144在高温下会开环产生新的物质;5F-UR-144从130℃开始分解,随着温度升高分解程度提升,240℃时分解率达到98%;随着温度继续升高,超过260℃,分解产物会碳化。结论基于5F-UR-144的热不稳定性,在检测时应考虑若通过烫食方式吸食5F-UR-144,其进入人体的成分会发生变化;气相色谱或气相色谱-质谱法不适合定量检测5F-UR-144。     

Forensic Imaging‐Guided Recovery of Nuclear DNA from the Spinal Cord*,†

Abstract: Our objective is to document the recovery of DNA from the spinal cord or surrounding dura mater in 11 cases of severely burned human remains. Radiographs established that portions of charred tissue contained spine segments. Multidetector computed tomography (MDCT) revealed that each spine specimen contained an intact spinal cord remnant. A full DNA profile was obtained from seven specimens using spinal cord dura mater in six specimens and spinal cord medulla in one specimen. A partial profile was obtained from four specimens (spinal cord dura mater, 2; spinal cord medulla, 2). Bone and muscle surrounding the spinal cord appear to insulate nucleic acid containing tissue from critical thermal degradation. The spinal cord, which is easily identified by MDCT examination of remains and easily recovered at the postmortem examination, can be a source of DNA with extraction yields comparable with other tissue sources. Specimens of dura mater are preferable as processing time is faster than bone.     

朝核问题视角下的东北亚安全

冷战后,自从朝核问题出现以来,东北亚的安全局势发生了巨大的变化,朝核问题已然成为东北亚安全的焦点与核心。基于东北亚的区域特点和安全形势,朝核问题的地区解决方式应该是比较稳妥、长远、符合现实的,同时需要地区内主要大国的推动以及双边与多边机制的结合,重要的是在区域的核心点上将朝鲜纳入国际社会的发展轨道。然后,在这些原则基础上去探索东北亚安全机制建构的路径。     

Perception and Politics in Intelligence Assessment: U.S. Estimates of the Soviet and "Rogue-State" Nuclear Threats

United States estimates of Soviet nuclear goals and capabilities and the current "rogue-state" nuclear threat reflected prevailing beliefs about threat within the U.S. government and the relative influence of agencies charged with threat assessment. This article establishes that the patterns in formal Soviet threat assessment: (i) did not reflect a uniform response to "external threat," (ii) were inevitably tied to underlying assumptions about adversary intent, and (iii) were susceptible then to perceptual, organizational, and/or political influences within government. Thus, threat assessments reflected the optimism and pessimism—and political interests and ideologies—of those who participated in the estimating process. The article concludes by examining these lessons in light of the experiences and challenges of assessing threat from small states harboring nuclear ambitions.     

The United States Congress and nuclear war powers: explaining legislative nonfeasance

Scholarly debate over the role of the United States Congress in approving military action has focused on the respective war powers granted the executive and legislature by the United States Constitution. Although a voluminous literature has examined the institutional and partisan politics shaping their exercise, a conspicuous lacuna concerns nuclear war powers. Despite periodic but mostly ineffective reassertions of congressional prerogatives over war, the decision to employ nuclear weapons has been left entirely to presidential discretion since 1945. Explaining this consistent refusal by Congress to rein in the ultimate presidential power and exercise co-responsibility for the most devastating form of war relies less on disputatious constitutional grounds than on three arguments about congressional dysfunctionality, legislative irresponsibility, and the relative costs of collective action by federal lawmakers on perilous national security questions.     

实验性脑挫伤GFAP,PCNA免疫组织化学研究

采用改进的Ｆｅｅｎｅｙ氏落体打击致Ｗｉｓｔａｒ大鼠脑挫伤模型,利用免疫组织化学染色,观察大鼠实验性脑挫伤后ＧＦＡＰ、ＰＣＮＡ的改变,用图像分析仪对免疫组化染色阳性细胞灰度和面积进行测量,ＳＡＳ统计软件分析,得出脑挫伤后ＧＦＡＰ、ＰＣＮＡ变化的时间规律性,为脑挫伤形成时间推断提供新的手段。ＧＦＡＰ阳性细胞面积和灰度伤后呈上升趋势,伤后３ｈ,即有显著增加（Ｐ＜０．０１）,至４天时,阳性细胞灰度,面积达到峰值（Ｐ＜０．０１）,伤后７天仍保持较高水平（Ｐ＜０．０１）。ＰＣＮＡ阳性细胞伤后１２ｈ出现,灰度呈上升趋势,面积呈下降趋势,伤后３天时达到最大值（Ｐ＜０．０１）。因此,ＧＦＡＰ、ＰＣＮＡ免疫组织化学染色阳性细胞的数量,灰度,面积改变有时间性规律;推断２～７天的脑挫伤则以ＧＦＡＰ、ＰＣＮＡ改变为主;脑挫伤后星形胶质细胞数目增多,只有很少一部分为星形胶质细胞增殖而来,且这种增殖在伤后３天时达到高峰     

A Review of: “Devin R. Springer,James L. Regens,and David N. Edger. Islamic Radicalism and Global Jihad. Stephen Vertigans (with contributions from Donncha Marron and Philip W. Sutton). Militant Islam: A Sociology of Characteristics,Causes, and Consequences. David Aaron. In Their Own Words: Voices of Jihad.”

Chinese arms sales to Africa have increased in recent years. In a region beset by conflict and unstable regimes, and where arms sales are a significant and positive predictor of an increased probability of political violence, this is inherently problematic. The sale of weaponry to a regime in Khartoum caught up in an alleged “genocide” in Darfur, the awkward appearance in 2008 of a Chinese ship loaded with weapons bound for Mugabe's Zimbabwe off the coast of eastern Africa, and the recent exposure in 2011 that Chinese arms companies offered to sell around $200 million worth of arms to Muammar Gaddafi's regime are emblematic of an issue in Africa's political violence that needs analysis. This article seeks to discuss the rationale behind China's arms sales to Africa and the effect that they have had on political violence in recipient countries. It also provides an analysis of the supply-and-demand circumstances of Chinese arms transfers to Africa, Beijing's attempts to control such transfers, and evidence that Chinese policies on proliferation are (slowly) evolving.     

US state crimes related to nuclear weapons: Is there hope for change in the Obama administration?

Given the long history of US state crimes related to nuclear weapons and the aggressive unilateralism of the George W. Bush administration that compounded these crimes, the election of Barack Obama created a “hope” for “change” in American nuclear weapons policy. While it is too early to render any conclusive judgment, we offer a preliminary assessment of the Obama record with regard to nuclear weapons based on a number of significant policy statements made and official actions taken, including Obama's 2009 Prague speech, the signing of the new START agreement in April 2010, the administration's 2010 Nuclear Posture Review, the Washington DC Nuclear Security Summit, and the Nuclear Nonproliferation Treaty Review Conference of 2010. Although the new administration has taken some steps to reduce the likelihood of the use or threat to use nuclear weapons, we conclude that under President Obama the US continues to be in violation of the solemn legal obligation to disarm as imposed by the NPT treaty of 1968. We also briefly note some of the structural and cultural factors related to the American empire that hinders any president from changing American nuclear weapons policy.     

SNRPN基因rs220030位点的分型及甲基化亲缘相关性

目的建立变性梯度凝胶电泳(denaturing gradient gel electrophoresis,DGGE)技术与焦磷酸测序技术对小核核糖核蛋白多肽N(small nuclear ribonucleoprotein polypeptide N,SNRPN)基因rs220030位点的分型方法。建立应用焦磷酸测序技术分析CpG甲基化状态的方法,探讨rs220030位点用于亲缘等位基因判定的可行性。方法应用DGGE技术对97例上海地区汉族家系血样rs220030位点进行分型,同时应用焦磷酸测序技术对其中25例血液来源的家系样本的rs220030位点分型,并对两种方法在SNP分型结果上进行比较。通过重亚硫酸盐修饰联合焦磷酸测序技术分析随机2组家系样本rs220030位点上游CpG甲基化状态,判断甲基化是否有亲缘相关性。结果经DGGE检测97例家系血样rs220030位点分型结果为C纯合子20例,T纯合子29例,C/T杂合子48例。经焦磷酸测序检测25例血液来源的家系样本结果与DGGE检测结果一致。经重亚硫酸盐修饰联合焦磷酸测序技术分析,2组血液来源的家系子代的rs220030位点上游CpG甲基化状态均与母亲较相似。结论相比DGGE技术,焦磷酸测序技术更精确、方便,适合大样本、高通量SNP分型。重亚硫酸盐修饰联合焦磷酸测序技术可以精确分析CpG甲基化状态。rs220030位点可用于亲缘等位基因判定。     

A Nuclear-Free Middle East – Just Not in the Cards

A scheduled conference to promote a zone free of weapons of mass destruction in the Middle East has renewed hopes for nuclear disarmament in this unstable region, if only innovative diplomacy could take advantage of the current shifts. However, a realistic assessment suggests that optimism is unwarranted. Fundamental strategic considerations related to Iran's nuclear program, Israel's atomic options, and the region's ingrate security architecture remain nearly insurmountable hurdles. Therefore, policymakers should focus first on attaining a resolution of the Israeli-Palestinian conflict.