Mismatched multiplex PCR amplification and subsequent RFLP analysis to simultaneously identify polymorphisms of erythrocytic ESD, GLO1, and GPT genes

ESD (esterase D), GLO1 (glyoxalase I), and GPT (glutamate pyruvate transaminase) are human erythrocytic isoenzymes and have previously been applied in forensic medicine caseworks. The molecular bases of the polymorphic gene expression products have been demonstrated to be because of SNPs in respective coding regions. However, it has not been revealed whether the SNPs conferring the polymorphisms to the aforementioned erythrocytic isoenzymes could be simultaneously detected by using a simple PCR method. In this study, we used mismatched primers to simultaneously amplify three common isoenzyme loci so that all amplified products contained the same Hph I cleavage sites. The products were then digested with Hph I and electrophoretically separated and stained so that alleles were identified. The accumulated values for the probability of discrimination power and excluding the probability of paternity to the aforementioned systems attained 90.41% and 41.72%, respectively, in the Chinese Han population. This assay could be extremely valuable for future forensic medicine practices.     

从宁波工艺品公司案看我国法院对涉外仲裁协议的监督

国家法院对仲裁协议实施监督的核心问题,就是仲裁协议的效力和仲裁庭管辖权的认定：在仲裁程序开始之前,法院就应当根据另一方当事人的请求,令当事人将协议项下的事项提交仲裁解决,除非法院认定仲裁协议无效、失效,或者不能履行;在仲裁程序开始之后,法院仍然有权根据一方当事人的请求对仲裁协议有效性和仲裁庭的管辖权问题进行审理,法院的此项权力来源于法院地国的国内法;在仲裁程序结束之后,当事人还可以裁决所依据的仲裁协议无效为由,申请法院撤销此根据无效仲裁协议作出的裁决,或者拒绝承认与执行此裁决。但如果当事人在仲裁程序中放弃了对仲裁协议有效性或者仲裁庭的管辖权提出异议的权利,则当事人在裁决作出就不能再以仲裁协议无效或者仲裁庭对仲裁协议项下的争议没有管辖权为由,请求法院撤销或者拒绝执行此仲裁裁决。     

Y-chromosome and autosomal STR diversity in four proximate settlements in Central Anatolia

Due to the longstanding human presence in the region and the influence of social traditions, the genetic make-up of populations currently inhabiting Turkey (Anatolia) is quite complex. To characterize the patterns of genetic diversity in rural Central Anatolian villages, we analyzed samples collected at four local settlements for variation at 17 Y-chromosome STR and 15 autosomal STR loci. The resulting data reveal considerable diversity within these settlements, as well as some structure in the paternal genetic variation, with a limited number of haplotypes being shared between the communities. These findings have important implications for forensic studies of Turkish populations.     

Simultaneous Identification of Four “Legal High” Plant Species in a Multiplex PCR High‐Resolution Melt Assay,

The international prevalence of “legal high” drugs necessitates the development of a method for their detection and identification. Herein, we describe the development and validation of a tetraplex multiplex real‐time polymerase chain reaction (PCR) assay used to simultaneously identify morning glory, jimson weed, Hawaiian woodrose, and marijuana detected by high‐resolution melt using LCGreen Plus^®. The PCR assay was evaluated based on the following: (i) specificity and selectivity—primers were tested on DNA extracted from 30 species and simulated forensic samples, (ii) sensitivity—serial dilutions of the target DNA were prepared, and (iii) reproducibility and reliability—sample replicates were tested and remelted on different days. The assay is ideal for cases in which inexpensive assays are needed to quickly detect and identify trace biological material present on drug paraphernalia that is too compromised for botanical microscopic identification and for which analysts are unfamiliar with the morphology of the emerging “legal high” species.     

心脏肌钙蛋白T、I与心肌损伤及其法医学应用前景

心脏肌钙蛋白T、I具有很高的心脏特异性,敏感性高,能检测多种不同的心肌损伤,区别心肌损伤和骨骼肌损伤,并能用作心肌损害的危险程度分级和判断预后的指标,在临床上得到了广泛的应用。在法医学上也有一定的应用前景。     

走新型工业化道路：科技进步与产业结构优化

走新型工业化道路,应在保证传统产业的支撑和基础地位的条件下,优先发展高新技术产业,全面发展第三产业。     

论安娜·卡列宁娜的悲剧心理

安娜.卡列宁娜是托尔斯泰创造的一个为了追求爱情自由而与整个上流社会抗争并最终以死的悲剧收场的妇女形象。根据安娜所处时代、生活环境、自身特性的心理探讨,可以探究其最终卧轨自杀的悲剧的起源、时代性、自身性以及终结等几个方面的意义。     

先秦儒家思想视野下的生态伦理观及现代价值

先秦儒家天人合一的生态哲学、仁民爱物的生态伦理情怀、取物有时有节的生态伦理实践精神以及制用天命的生态保护原则,在我国古代生态伦理思想中产生了深远而重大的影响。深入挖掘和梳理阐释先秦儒家生态伦理思想,对于进一步提高人们的环境道德自觉意识、构建节约型社会及建设生态文明具有重要的理论意义和实践价值。     

莱芜梆子现代爱情戏浅谈

莱芜梆子是第二批国家级非物质文化遗产,它自形成以来,一直是莱芜文化的象征。其现代戏折射出了当今丰富多彩的社会生活,而爱情戏作为现代戏的一个重要组成部分,与传统戏相比有了全新的发展,莱芜梆子现代爱情戏突破了历来的“才子佳人戏”的樊篱,紧扣时代主题,体现了鲜明的“现代性”特征。我们将从“忠于政治”、“融入亲情”、“秉承孝道”、“源自义举”四个方面阐释莱芜梆子现代爱情戏的内容,并在与传统戏的比照中突显现代爱情戏的特色。     

Sequences of the Cytochrome C Oxidase Subunit I (COI) Gene are Suitable for Species Identification of Korean Calliphorinae Flies of Forensic Importance (Diptera: Calliphoridae)

Abstract:  Calliphorinae fly species are important indicators of the postmortem interval especially during early spring and late fall in Korea. Although nucleotide sequences of various Calliphorinae fly species are available, there has been no research on the cytochrome c oxidase subunit I (COI) nucleotide sequences of Korean Calliphorinae flies. Here, we report the full-length sequences of the COI gene of four Calliphorinae fly species collected in Korea (five individuals of Calliphora vicina , five Calliphora lata , four Triceratopyga calliphoroides and three Aldrichina grahami ). Each COI gene was amplified by polymerase chain reaction and directly sequenced and the resulting nucleotide sequences were aligned and analyzed by MEGA4 software. The results indicate that COI nucleotide sequences can be used to distinguish between these four species. Our phylogenetic result coincides with recent taxonomic views on the subfamily Calliphorinae in that the genera Aldrichina and Triceratopyga are nested within the genus Calliphora .