谈科技期刊选题优化的基本原则

优化科技期刊选题，一方面能够使科技信息传播变得有序化．增强针对性；另一方面，能够引导科学研究向纵深发展．促进科技进步和社会发展。基于此．在选题策划过程中应结合可能出现的新情况，遵循引导性、前瞻性、针对性、效益性和可行性基本原则．随时对选题进行修缮．使更好地发挥科技期刊的作用。     

加快生态文明建设，实现科学跨越发展

该文分析了中国实现科学跨越发展所面临的压力与挑战，指出建设生态文明对于应对这些问题具有重要意义。在此基础上，作者阐述了生态文明建设的内涵、目标与具体举措，为加快中国从工业文明到生态文明这一历史性转变、实现科学跨越发展提出对策。     

Developmental Validation of the Quantifiler® Duo DNA Quantification Kit for Simultaneous Quantification of Total Human and Human Male DNA and Detection of PCR Inhibitors in Biological Samples*

Abstract: The Quantifiler^® Duo DNA Quantification kit enables simultaneous quantification of human DNA and human male DNA as well as detection of inhibitors of PCR in a single real-time PCR well. Pooled human male genomic DNA is used to generate standard curves for both human (ribonuclease P RNA component H1) and human male (sex determining region Y) specific targets. A shift in the cycle threshold (C_T) values for the internal positive control monitors the presence of PCR inhibitors in a sample. The assay is human specific and exhibits a high dynamic range from 0.023 to 50 ng/μL. In addition, the multiplex assay can detect as little as 25 pg/μL of human male DNA in the presence of a 1000-fold excess of human female DNA. The multiplex assay provides assessment of the DNA extract and guidance for the selection of the appropriate AmpFℓSTR^® Amplification Kit to obtain interpretable short tandem repeat profiles.     

A comparison of methods used in the UK and Ireland for the extraction and detection of semen on swabs and cloth samples

The recent formation of a United Kingdom and Irish working group, the Body Fluids Forum (BFF), highlighted the need to investigate different working practices prior to any inter-laboratory comparison work and identification of best practice. Various dilutions of semen were seeded onto swabs and cloth samples for each BFF member laboratory to test using their standard techniques. The results showed that the detection of acid phosphatase on swabs is best achieved using direct testing rather than on an extract from the swab. Extraction methods for spermatozoa require a balance to be achieved between using a sufficient volume of water to ensure optimal release and minimal volume to ensure a concentrated extract. PSA tests were investigated and found to be more sensitive than Choline. DNA profiles were obtained from samples in which no spermatozoa had been detected during microscopic examination.     

法庭科学实验室认可工作发展状况与启示

本文通过对国内外,特别是对先进国家法庭科学实验室认可工作发展变化情况的介绍,分析我国认可工作中存在的不足,并提出改进意见。     

论股东劳务出资的可行性及其法律规制——以法经济学为视角

劳务出资是指股东以精神上和身体上的劳务作为出资取得股东身份。我国公司法修订后,劳务出资问题被推到了风口浪尖之上,成为理论界的一个热点问题,众多学者对此各抒己见,形成了不同的观点。文中从一个全新的角度——法经济学的视角出发,从理性人假设、博弈论分析、守法成本等几个方面对劳务出资的合理性、运用法律对劳务出资进行规制的必要性和劳务出资法律规范的选择等问题进行了分析和论证。     

高职计算机专业举办技能大赛的实践探索

结合高职计算机专业特点,依据岗位技能,开展丰富多彩的技能大赛,激发学员的自主学习兴趣,是提高高职院校人才培养质量的有效途径。文章通过近年来开展计算机相关专业技能大赛的实践,就举办技能大赛的内容选择、组织形式选择以及校企合作等方面,分析了如何利用技能大赛这种方式,提高学员的实践技能水平。提出依据专业特点、结合岗位技能分析、采纳合作单位的意见来优选技能大赛内容,同时通过技能大赛的形式提高学员的综合技能。     

树立科学发展的科技观刍议

树立科学发展的科技观,就是要坚持以人为本的思想,满足全面协调可持续的基本要求和统筹兼顾的根本方法,正确处理好我国科技事业发展中的各种复杂关系,实现我国科技事业总体布局及各环节的共同、均衡和协调发展,真正使我国的科技事业走上全面协调可持续发展的新道路。     

抗丁丙诺啡单克隆抗体的制备

目的建立抗丁丙诺啡单克隆抗体的杂交瘤细胞株，制备高特异性的丁丙诺啡单克隆抗体，并对其免疫学特性进行鉴定。方法在丁丙诺啡的分子上连接活性羧基基团，通过缩合反应将丁丙诺啡半抗原连接于血蓝蛋白（KLH）和小牛血清白蛋白（BSA），形成完全抗原。以完全抗原免疫Balb／c小鼠，通过细胞融合，筛选等杂交瘤技术，建立稳定的分泌抗丁丙诺啡单克隆抗体的杂交瘤细胞株。通过腹腔注射杂交瘤细胞，诱导小鼠产生含有单抗的腹水。用辛酸-硫酸铵加亲和层析法纯化抗丁丙诺啡单克隆抗体。采用酶联免疫反应和胶体金膜层析实验测定丁丙诺啡单抗的特异性以及免疫反应动力学参数。结果共获得3株分泌抗丁丙诺啡单克隆抗体的杂交瘤细胞株，分别命名为7E6，6G4和3C2。7E6，6G4抗体灵敏度为10．0ng／ml，3C2抗体灵敏度为20．0ng／ml。7E6，6CA和3C2抗体的亲和常数分别为3．6×10^-9 mol／L，4．3×10^-9 mol／L和6．3×10^-9 mol／L。特异性测试结果表明7E6和6G4抗体与40种药物、毒品无任何交叉反应，而3C2抗体与吗啡有交叉反应。结论杂交瘤细胞株7E6和6G4产生的抗丁丙诺啡单克隆抗体具有很高的特异性和灵敏度。     

DNA typing in populations of mule deer for forensic use in the Province of Alberta

The present study involves the development of forensic DNA typing tests and databases for mule deer in the Province of Alberta. Two multiplex PCR reactions interrogating 10 loci were used to analyze samples from three populations of mule deer. Additionally, an amelogenin based sex-typing marker was used to determine the gender of samples. Results show that the tests and databases are appropriate for use in forensic applications. Additionally, the results indicate that there is little population structure in mule deer in Alberta and that no changes to management of this game species are suggested.