GC–MS analysis of eight aminoindanes using three derivatization reagents

Aminoindanes are a class of novel psychoactive substances (NPSs) that have become more prevalent over the past decade. GC–MS is often utilized for identifying seized drugs and is well regarded for its ability to separate mixtures. However, certain aminoindanes have similar mass spectral data and require specific gas chromatographic stationary phases for separation. Derivatization is an alternative method that can be applied to GC–MS to enhance chromatographic results, providing more selective analysis in seized-drug identification. This study investigates derivatization techniques to provide options for forensic science laboratories in accurately identifying aminoindanes. Three derivatization reagents, N-methyl-bis(trifluoroacetamide) (MBTFA), heptafluorobutyric anhydride (HFBA), and ethyl chloroformate (ECF) were evaluated for the analysis of eight aminoindanes by GC–MS using two common gas chromatographic stationary phases, Rxi®-5Sil MS and Rxi®-1Sil MS. All three derivatization methods successfully isolated eight aminoindanes, including the isomers 4,5-methylenedioxy-2-aminoindane (4,5-MDAI), and 5,6-methylenedioxy-2-aminoindane (5,6-MDAI) that could not be differentiated prior to derivatization. Reduced peak tailing and increased abundance were observed after derivatization for all the compounds, and mass spectra of the derivatives contained individualizing fragment ions that allowed for further characterization of the aminoindanes. This excluded 4,5-MDAI and 5,6-MDAI as they shared the same characteristic ions and were only distinguishable by their retention times. All three derivatization techniques used in this study allow for successful characterization of the aminoindanes and give forensic science laboratories flexibility in their analysis approach when they encounter these compounds.     

Synthesis,structure, in vitro pharmacology,and in vivo activity of trans-3,4-dichloro-N-[[1-(dimethylamino)-4-phenylcyclohexyl]methyl]-benzamide (AP01; 4-phenyl-AH-7921), a novel mixed μ-/κ-opioid

Analogs of non-fentanyl novel synthetic opioids (NSO) with modifications that fall outside of established structure–activity relationships (SARs) for that class of drugs create the question whether or not it should be considered an analog, as defined by 21 U.S.C. §802(32)(A), which is important for its inclusion in the US system of drug scheduling. AH-7921 is a US Schedule I drug and an example of the 1-benzamidomethyl-1-cyclohexyldialkylamine class of NSO. The SARs regarding substitution of the central cyclohexyl ring have not been well characterized in the literature. Therefore, in order to expand the SAR surrounding AH-7921 analogs, trans-3,4-dichloro-N-[[1-(dimethylamino)-4-phenylcyclohexyl]methyl]-benzamide (AP01; 4-phenyl-AH-7921) has been synthesized, analytically characterized, and tested in vitro and in vivo pharmacologically. Using methods described in the original patents for this class of NSO, it was found that the single trans geometric isomer was obtained. The proton nuclear magnetic resonance, mass spectrum, infrared spectrum, and Raman spectrum are reported along with the melting point of the hydrochloride salt. In vitro binding to a battery of 43 central nervous system receptors showed it to be a high-affinity μ-opioid receptor (MOR) and κ-opioid receptor (KOR) ligand (60 nM and 34 nM, respectively). AP01 also had a 4 nM affinity for the serotonin transporter (SERT), which is a higher level of potency at this receptor than most other opioids. In rats, it exhibited antinociception in the acetic acid writhing test. Therefore, the 4-phenyl modification results in an active NSO, but carries with it potential toxicities beyond those expected for currently approved opioid drugs.     

Pressure sensitive adhesives and paper spray-mass spectrometry for the collection and analysis of fentanyl-related compounds from shipping materials

The rise of fentanyl and fentanyl analogs in the drug supply pose serious threats to public health. Much of these compounds enter the United States through shipping routes. Here we provide a method for fentanyl screening and analysis that utilizes pressure-sensitive adhesive (PSA) lined paper to recover drug residues from parcel-related surfaces. The paper used is commercially available repositionable notes (also called post-it or sticky notes). From this paper, mass spectra were obtained by paper spray-mass spectrometry (PS-MS), where PSA paper served as both a sampling and analysis substrate. Seven fentanyl-related compounds were analyzed: fentanyl, 4-anilino-N-phenethylpiperidine (4-ANPP), N,1-diphenethyl-N-phenylpiperidin-4-amine (phenethyl-4-ANPP), valerylfentanyl, 4-fluoroisobutyrylfentanyl (4-FIBF), carfentanil, and p-fluorofentanyl. These compounds were recovered by PSA paper and identified by PS-MS from packaging tape and plastic at 50 ng and from cardboard and shipping labels at 100 ng. The impact of cutting agents on PS-MS analysis of fentanyl analogs was explored. No trends of analyte suppression were found at high concentrations of the cutting agents caffeine, diphenhydramine, and lidocaine when recovered from surfaces. A cartridge that required no precise cutting of PSA paper prior to sampling or analysis was evaluated for use in PS-MS for fentanyl screening. Recovery and detection of fentanyl from plastic sheeting was demonstrated with this cut-free cartridge. The cut-free cartridge showed somewhat less consistency and lower analyte signal than the standard cartridge, but performance was suitable for potential screening applications. In combining PSA surface sampling with PS-MS for drug screening, both sampling and detection of fentanyl-related compounds is simple, rapid, and low-cost.     

王安忆小说的叙事建构及读解

王安忆是个高产作家,其创作不仅高产而且有质量,因而吸引了许多评论者的目光,但对王安忆小说创作进行总体研究难度甚大。将王安忆小说文本的叙事修辞与作家的叙事理念相结合,在对其小说文本的叙事探究中注入修辞分析的元素,从修辞对话的层面阐释其小说创作,不失为一个新的视角。     

论从民俗文化视角研究莫言小说的意义

莫言小说创作深受民俗文化的影响。民俗文化既是莫言小说创作的素材,又是莫言小说创作的源泉。因此,从民俗文化角度研究莫言小说创作具有非常重要的意义:从民俗文化的视角探讨莫言小说的创作,可以拓宽莫言小说研究的视野;通过揭示民俗文化对莫言的影响,探索莫言小说创作的源泉;莫言清醒的民俗学意识影响和制约着他的小说理论和创作思维,决定着小说的民俗化倾向;通过分析民俗文化在莫言小说中的影响、表现及其作用,探讨莫言小说中的意象所蕴涵的象征意蕴。     

Poor communities as corporate stakeholders: A bottom-up research approach

Abstract

This article strives to provide an understanding of salient issues affecting the daily lives of participants from various developing communities in the country, and within the bigger picture, discuss some implications for organisations that affect or are affected by such communities. A key implication is that the process of constant connectivity and dialogue, including dissent, with communities as corporate stakeholders, may be more important in establishing trust and earning accountability, than the outcomes of well-planned corporate social responsibility campaigns. The study is based on qualitative research undertaken between 2006 and 2008 in 35 South African rural and township communities in Limpopo Province, North-West and Gauteng. A bottom-up research approach was proposed by the researchers, which, instead of evaluating the effects of corporate communication campaigns on communities, was to begin at a grassroots level with communities themselves, by exploring top-of-mind issues. From the findings it was apparent that a vicious cycle of extreme and endemic poverty was the focal area that occupied community members’ minds. This study provides a linkage between certain aspects of corporate social responsibility, normative stakeholder theory, strategic communication and stakeholder dialogue, in an attempt to provide organisations with guidelines to evaluate and respond to the challenges of poor communities, and offer a perspective on the way strategic communication with poor communities should take place.     

Effect of time and relationships on victim–offender dialogue completion in felony cases

Victim–offender dialogues (VODs) often take place in organizational contexts, the stakeholders of which may very well be interested in measures of program effectiveness such as completion rates. When reported, completion rates typically ranged from 40 to 60%. At the time of this study, Ohio’s VOD program was completing just 25% of initiated cases and program stakeholders were unsure as to the cause(s). An archived data analysis was performed on a sample (n?=?212) of the Office of Victim Services (OVS) completed and will-not-proceed files. One hypothesis and two research questions make use of archived data to explore this felony VOD context. The amount of time between the date the crime occurred and the date on which the dialogue file was initiated was not a significant predictor of dialogue completion. However, both victim-offender’s pre-crime relationship and dialogue file initiator were found to significantly impact dialogue completion rates. These results are considered in light of social exchange and uncertainty reduction theories.     

The reluctant activist: the Italian parliament and the scrutiny of EU affairs between institutional opportunities and political legacies

ABSTRACT

The article analyses the participation of the Italian Parliament in the scrutiny of EU affairs after the entry into force of the Treaty of Lisbon and its implementation through the national Law 234/2012. The empirical analysis highlights that notwithstanding the presence of favourable institutional and political conditions, the involvement of the Italian Parliament in EU affairs moderately increased. The Treaty of Lisbon and Italian legislation improved Parliament’s rights to participate in the ascending phase but without altering significantly the balance of powers between the European Commission and NPs, and between the Italian Parliament and the Government. Moreover, MPs perceive the EWS and the PD as not impactful on decision-making at the EU and the national level.     

Remembering violence: the role of apology and dialogue in Turkey's democratization process

In this article I ask the question: how do citizens use memories of violence in dialogue with a democratizing Turkish state? To address this, I unpack how memories of violence influence solidarity communities in addition to those who are direct descendents of survivors. I also examine how these solidarity communities are widening political space for contemporary dialogue about the Armenian Catastrophe. To demonstrate the connection between memory and political participation, I identify three discursive moments where Turkish and Armenian citizens invoke memory in dialogue with one other and with the state. I use the 2009 online campaign for a Turkish apology to address the Armenian Catastrophe, the aftermath of the murder of Hrant Dink in 2007, and a controversial 2005 academic conference on the events of 1915 as focal points to discuss how memory impacts the way people behave as citizens. My argument is twofold: first, elite-led solidarity networks play an integral role in shaping the discursive space between citizens, the state, and the international community; and second, dialogue about memory can grow space for citizen participation in Turkey.     

台湾都市文学与海派文学

本文以林燿德和施蛰存开启的台湾都市文学与海派文学的对话为契机,探讨海峡两岸都市文学的隔海传承与变异。30年代上海新感觉派将都市文化的殖民地摩登属性推向顶峰;40年代的海派作家还原出一个琐碎的市民都市空间;台湾新世代作家则塑造了一个集民间、私我和异彩文化等诸多因子交汇的都市场域,包容了更大的阐释空间。这些都市书写都以“感觉”为媒介,借鉴蒙太奇手段刻意颠覆故事情节的连贯性和人物性格的完整性,对欲望书写异乎寻常的关注,在小说的审美形态、叙述角度、结构方式、语言风格等方面,表现出特有的创新度和开放度。