Simplified Direct PCR Method for Reference Buccal Samples Using a Non-FTA Card by Omitting the Pretreatment Step

When using non-FTA cards in commercial multiplex STR kits for direct PCR, pretreatment steps with specific buffers are recommended. Here, we designed a rapid direct PCR method utilizing a non-FTA card, Oral Cell Sampling Kit, by omitting the pretreatment step involving Prep-n-Go™ Buffer, and it showed compatibility with the GlobalFiler™ Express PCR Amplification Kit, GlobalFiler™ PCR Amplification Kit, and PowerPlex^® Fusion system. To optimize the PCR conditions, we tested the method with different final PCR volumes and cycles. Finally, we conducted a performance test using 50 Korean buccal samples and confirmed the high performance of the method, detecting more than 90% of the samples with full profiles when using GlobalFiler™ PCR Amplification Kit and PowerPlex^® Fusion system at 29 cycles in a 10 μL final PCR volume. Thus, we report a simple direct PCR set-up to analyze reference samples collected using a non-FTA card manufactured in Korea.     

How do coalition signals shape voting behavior? Revealing the mediating role of coalition expectations

Existing empirical research suggests that there are two mechanisms through which pre-electoral coalition signals shape voting behavior. According to these, coalition signals both shift the perceived ideological positions of parties and prime coalition considerations at the cost of party considerations. The work at hand is the first to test another possibility of how coalition signals affect voting. This coalition expectation mechanism claims that coalition signals affect voting decisions by changing voters' expectations about which coalitions are likely to form after the election. Moreover, this paper provides the first integrative overview of all three mechanisms that link coalition signals and individual voting behavior. Results from a survey experiment conducted during Sweden's 2018 general election suggest that the coalition expectation mechanism can indeed be at work. By showing how parties' pre-electoral coalition behavior enter a voter's decision calculus, the paper provides important insights for the literature on strategic voting theories in proportional systems.     

Small winners and big losers: strategic party behaviour in the 2017 Dutch general election

Abstract

This article analyses party strategies during the campaign for the Dutch general election of March 2017, making use of issue-yield theory. It investigates whether parties strategically emphasise high-yield issues, by juxtaposing the issue opportunities provided by voters with parties’ issue emphasis during the campaign. More specifically, it asks whether parties strategically emphasised issues that were expected to reward them electorally. Analysing voter preferences and party campaign data, it is found that parties and most of their constituencies show high ideological consistency, that parties emphasise mostly positional issues and thus choose a conflict-mobilising strategy, and that most parties emphasise high-yield issues rather than following the general political agenda. Four small parties that won significantly behaved strategically while the social democrats – who severely lost – hardly did. The findings imply that the issue-yield framework can help to explain the election result in the fragmented Dutch multi-party context.     

‘Get Brexit Done’: The New Political Divides of England and Wales at the 2019 Election

This article analyses the successful Conservative election campaign of 2019 and how it took advantage of a fractured political and economic landscape. It reviews the unique circumstances around the 2019 election and the ‘surprising death’ of a no-deal Brexit. We then analyse the divergent political communication strategies in the 2017 and 2019 Conservative campaigns showing how the latter was much more coherent and politically unorthodox. Drawing on socioeconomic, demographic and British Election Study data, we argue that Boris Johnson’s messaging was carefully tailored towards the demands of voters in the ‘red wall’ seats. Conservative success was built around an appeal to voters in these economically depressed ‘geographies of discontent’. But while tremendously successful, the coalition this created is potentially fragile. An unconventional, ‘leftish’ Conservative campaign built a new, diverse bloc of voters. It includes a number of left-wingers expecting change alongside traditional Conservative supporters, and will be hard to keep together given the economic turbulence ahead.     

Beyond Political Binaries: The 2019 General Election and the Dagenham and Rainham Constituency

This article considers the results of the 2019 general election with reference to the Dagenham and Rainham constituency in outer East London. It was a key target for the Conservatives with a 70 per cent leave voting electorate. It did not change hands and might therefore provide insights into the wider debate regarding future coalitions and strategy within the modern left. This article considers these results with reference to arguments about a ‘Brexit realignment’ on the left and whether Labour should rethink the nature of its political ‘base’. It argues for a more nuanced debate than that which currently exists, built around simple binaries organised around Brexit, class, age, education and geography.     

Two Contrasting Constituencies in the Heart of England

This article compares two parliamentary constituencies in Warwickshire: one which might be expected to be Conservative but is held by Labour, and the other, a Labour seat for over fifty years, but now held by the Conservatives. Constituency level analyses permit a more fine-grained analysis of electoral trends, taking account of demographic factors otherwise being overlooked. Labour needs to be able to appeal to the aspirational voters moving into Nuneaton.     

外商直接投资区位理论综述

对外直接投资在二战后发展的规模和速度极为突出。作为国际生产的主要载体,跨国公司在对外直接投资时．首先要考虑的一个重要问题就是区位选择问题。跨国公司直接投资的区位因素分析,一直是国际经济学界关注的理论热点之一。在经济全球化背景下,研究跨国公司对外投资区位决策因素的变迁,对分析跨国公司的直接投资行为和投资流向的变化具有重要意义。     

Against Localism: Does Decentralising Power to Communities Fail Minorities?

The continued decline in levels of political engagement among British citizens has led many politicians, commentators and academics from across the political spectrum to advocate a move toward a more direct form of democracy via some kind of localism. The claim is that citizens feel increasingly estranged from the democratic process, and from those organisations on which they have historically relied to represent them within the political system. Consequently, localists argue, there now exists a gap between the people, the institutions which are supposed to work on their behalf, and the decisions made in their name, so the system needs to be reformed in such a way as to give individuals and local communities more of a direct input into the decision-making process. Calls for a more direct form of democracy via localism are popular among members of the progressive left and the 'new Conservative' right, and have become so dominant in political discourse that it is often suggested that 'we are all localists now'. This article raises questions about the localist agenda, and suggests that the adoption of a more direct form of democracy in Britain may not only fail to address the decline in political engagement, but may also result in the exclusion, marginalisation, and oppression of minority groups.     

村委会选举中的富人贿选现象分析

富人贿选是我国村民自治实践中不可回避的客观现实。富人贿选具有严重的社会危害性,其消极影响主要表现为：压制了农村的民主萌芽,损害了党和政府的形象,加剧了农村社会矛盾,造成了村民“政治冷漠”。结合我国村民自治的发展实践,要想使富人贿选现象得到有效的抑制,一要进一步加强村务公开和民主管理工作,二要不断完善相关法律制度,三要规范村委会成员候选人资格条件,四要加强对选举的指导和监督。     

Shifting rights,property and authority in the forest frontier: ‘stakes’ for local land users and citizens

Customary land and forests are more embedded in the global economy than ever. With globally significant supplies of land and raw materials and favorable terms for foreign investors, developing countries – particularly in Africa – have become increasingly attractive trade partners and destinations for investors. Increasing competition over land is placing new pressures on vast tracts of forest and woodland, areas often considered ‘under-utilized’ by national governments despite their critical role in supporting local livelihoods. While increased demand for primary agricultural, forest and mining commodities in the context of forest tenure reforms and decentralized decision-making could create unprecedented economic opportunities for forest-dependent communities, increased ‘stakes’ over forest resources and land will undoubtedly heighten governance challenges. This is in no small part due to the political dynamics of property, and to the role of the ‘recursive constitution of property rights and authority’ in the evolution of the modern nation-state. By identifying the social ‘stakes’ associated with different pathways through which sectoral and extra-sectoral commodities shape forests, this paper provides a conceptual framework for analyzing how shifting contours of rights, property and authority in the context of forest-related trade and investment shape human well-being for affected communities and the wider citizenry of host countries. It then illustrates the use of the framework through its application to two brief case studies from southern Africa: tobacco production in Malawi and copper mining in Zambia. It is hoped that this framework will provide a meaningful contribution to growing scholarship on the political dynamics of property, and implications for rights-based approaches to agricultural investment and large-scale land acquisitions.