牛传染性鼻气管炎抗体的纳米胶体金斑点免疫渗滤法检测

将无血清细胞培养的牛传染性鼻气管炎病毒(IBRV)抗原包被于硝酸纤维素膜,加入待检血清样品后,利用纳米胶体金标记的山羊抗牛IgG显色,建立了检测IBR抗体的纳米胶体金斑点免疫渗滤法检测试纸盒。整个试验过程仅需5min即可判断结果,与蓝舌病、牛赤羽病、牛地方流行性白血病、牛病毒性腹泻黏膜病、猪瘟、猪伪狂犬病和猪细小病毒病的阳性血清不发生交叉反应。将该法与用于IBR抗体检测的ELISA方法同时对300份临床牛血清样品进行IBR抗体检测,结果二者的阳性符合率达94.4%。结果表明,该法具有特异、敏感、快速可靠、效果直观、结果容易判断的特点,非常适用于IBR的早期诊断和流行病学调查。     

嗜水气单胞菌胶体金快速检测试纸条的研制

用柠檬酸三钠还原氯金酸制备胶体金颗粒,标记抗嗜水气单胞菌单克隆抗体并制备金标垫。将吸收垫、喷涂有抗嗜水气单胞菌单克隆抗体和羊抗鼠抗体的硝酸纤维素膜、金标垫及样品垫组装成免疫层析试纸条,建立嗜水气单胞菌的胶体金免疫层析快速检测方法。用灭活细菌与血清混合的模拟检测样本测定试纸条的特异性、灵敏度,结果显示:试纸条对豚鼠气单胞菌、温和气单胞菌、鳗弧菌、溶藻弧菌、荧光假单胞菌、副溶血弧菌等13种常见病原菌没有交叉反应,与嗜水气单胞菌有特异性反应,检测灵敏度为1×105CFU/mL,检测时间小于5min。所制备的嗜水气单胞菌胶体金免疫层析检测试纸条具有快速、简便、特异性高、适用于基层临床生产推广应用等优点。     

Money after Blockchain: Gold,Decentralised Politics and the New Libertarianism

ABSTRACT

Blockchain technologies are central to what has been described as a new ‘smart social contract’. With blockchain, individual cryptographic identity becomes the basis for new forms of money and for a whole suite of restructured social, political and financial transactions. But what do these developments signal for feminist engagements with the money economy? The transparency and pseudonymity that the blockchain provides has been welcomed as a ‘feminist weapon’. But the decentralised technology also legitimises many longstanding assumptions of libertarianism, especially competitive individualism, naturalised social inequality and the stability of value associated with the gold standard. Drawing on popular culture texts, Goldfinger and The Mandibles, this article considers this history, examining the gendered, racialised and sexualised discursive practices that attend representations of gold along with the ‘metallism’ surrounding blockchain-based cryptocurrencies in the contemporary conjuncture. By claiming to represent non-negotiable certainty derived from technology/nature rather than social convention, the fantasy of fundamental value returns, together with related associations of essentialism and authenticity, but anchored in this new context in the technocratic authoritarianism of FinTech. This is part of the background for the ‘new libertarianism’ whose ascendency now overshadows the neoliberalism that has been the focus of critical attention for some decades.     

胶体金免疫层析一步法快速检测G1m(3)因子

建立一种简便快速的胶体金免疫层析一步法 ,用于检测 G1m(3)因子。采用柠檬酸三钠还原法制备胶体金颗粒 ,标记抗人 G1m(3)单克隆抗体 ,研制出抗人 G1m(3)因子免疫层析检测试剂盒。样品的 G1m(3)因子 ,与测试条上的金标记抗体结合后沿着反应膜移动 ,再与膜上固相抗体结合形成肉眼可见的红色反应带。使用该方法可检出 10万倍稀释的血清样品 ,整个试验只需 5 min完成。对常见的 2 3种动物血 (痕 )检验 ,未出现交叉反应。在 10 0例样品的检测中 ,本方法的检测结果 ,与 Dot- EL ISA的检测结果的符合率为 10 0 %。该方法适用于法医物证快速检验。     

氯胺酮胶体金标记单克隆抗体免疫层析检测板的研制

目的建立一种简便快速检测氯胺酮的方法。方法将20nm胶体金颗粒标记的抗氯胺酮单克隆抗体,均匀浸在吸水玻璃纤维上,用点膜机将氯胺酮-BSA和纯化后的羊抗鼠IgG多克隆抗体在硝酸纤维薄膜分别划1mm宽的检测线(T线)和质控线(C线),然后依次将吸水玻璃纤维、硝酸纤维薄膜和吸水滤纸粘贴于白色的塑料片上,切成0.5 cm×10 cm大小,制成氯胺酮胶体金标记单克隆抗体免疫层析检测条,并组装成检测板,并检测其特异性和灵敏度。结果氯胺酮胶体金标记单克隆抗体免疫层析检测板在对46种药(毒)物的测试中,仅识别氯胺酮及其主要代谢产物,其准确性为97.6%,检测氯胺酮的阈值为1000ng/m l。结论氯胺酮胶体金标记单克隆抗体免疫层析检测板在5m in内可检出样品中的氯胺酮及其代谢物。     

非法经营黄金期货若干问题探讨——对上海松江“4·18”系列非法经营黄金期货案的思考

非法经营黄金期货也被称作"地下炒金",近年来发案率居高不下,司法机关在办理这类案件时也不断面临新的问题和挑战。司法机关可以在没有行政机关行政违法确认函的情况下,根据具体法律和事实,对交易是否是期货性质进行司法认定;也可以在没有书面或电子标准化合约的条件下,通过对整个交易过程的分析,可以得出其是否采用标准化合约的实质性判断。将已经查明的投资人实际投入的保证金数额乘以手数,作为非法经营的犯罪数额,是相对恰当的。     

我国地下炒金炒汇的法律规制

我国地下炒金炒汇屡禁不止的原因之一是法律规制手段乏力，主要表现在立法不完善、对国（境）外公司的市场准入审查不严、监管不给力等。其中，无人监管、多头监管和不服监管是监管中存在的重大问题。为了有效规制我国的地下炒金炒汇行为，必须坚持疏堵并举的原则，以完善立法为基础，改善监管为关键，探索合并监管、并表监管、分类监管等模式，同时，借鉴国外先进的法律制度。     

Information Transmission Among World Major Gold Futures Markets： Evidence From High Frequency Synchronous Trading Data

This study examines the international information transmission among three major gold futures markets namely New York Mercantile Exchange in division of Commodity Exchange （COMEX）, Multi Commodity Exchange （MCX）, and Tokyo Commodity Exchange （TOCOM）. The main concept of this research is no matter where gold futures traded, they share the same underlying asset. Two well-documented approaches, which are vector error correction model and information share, are utilized to measure the process of price discovery under this trivariate system. The uniqueness of this study is that it employs synchronous intraday time series which can mitigate the stale price problem from daily observations. The evidences indicate that the three gold futures prices are cointegrated and driven by the same fundamental factors. New arrival information disseminates efficiently among the three markets and the pricing information transmission among exchanges is very rapid. However, the lead-lag relationship among markets still exists with the dominance of COMEX gold futures as the centre of price discovery The US gold futures market is the most efficient in processing information. Its role on price discovery and information can be attributed to COMEX＇s massive trading volume.     

特异性可卡因核酸适体测试条的研制

目的筛选识别可卡因的特异性核酸适体,制备可卡因核酸适体测试条（盒）。方法通过SELEX技术筛选可卡因核酸适体,制备胶体金标记可卡因完全抗原（COC-BSA）,制作可卡因层析测试条,并对方法特异性、灵敏度、准确性等性能指标进行考察和验证。结果甲基苯丙胺等64种药品和毒品采用本文研制的可卡因测试条检测,结果均为阴性,无交叉反应;检测可卡因最低浓度在5ng/mL,比胶体金单克隆抗体方法检测高5倍;将测试条密封45℃烘烤1个月后,检测结果与未烘烤过的测试条检测结果一致;与GC/MS检测结果对照,准确性为100%。结论本文研制的可卡因核酸适体检测试条,性能稳定,特异性良好,灵敏性和准确性均可达到实际检测的要求,在毒品、毒物的检测中具有广阔的应用前景。     

弓形虫抗体免疫胶体金快速检测试纸条的研制

将样品垫(加样区)、包被了弓形虫排泄分泌抗原和胶体金结合物的结合垫、包被了弓形虫排泄分泌抗原和抗弓形虫抗体的硝酸纤维素膜、吸水垫依次首尾互相衔接粘贴在白色塑料板上,组装成检测弓形虫抗体的免疫胶体金快速试纸条。然后用其进行动物弓形虫病血清抗体检测,并用弓形虫IHA诊断试剂进行对照试验。经对已知弓形虫阴、阳性血清检测,与弓形虫IHA检测结果的符合率为82.5%,金标试纸条较弓形虫IHA诊断试剂能提前2 d检测到抗体。结果表明,检测弓形虫抗体免疫胶体金试剂(金标试纸条)的敏感度高于IHA。